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PDMS Microfluidic Chip Design
PDMS Microfluidic Chip Design
The chosen design for our project incorporates three microfluidic chips, created out of two 3"x1" glass slides and a cut-out of PDMS to create a chamber for the cells. A 0.3 mm tubing was inserted through the PDMS. The day prior to the experiment, we must culture the cells and allow them to properly adhere to the cell microfluidic chips.
Clamping Seal
Clamping Seal
Using binder clips, we seal the chamber so it is air-tight.
Parts of Microfluidic Chip
Parts of Microfluidic Chip
The chip consists of a pre-made PDMS assembly (with tubes inserted and sealed on both sides), sandwiched between two glass slides. This setup creates an easy to use chip assembly/disassembly method - along with easy cleaning.
Benefits of glass slides:
Easier seeding of cells
Imaging abilities
Compatible with existing histology software
Affordable
Common to most labs
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