Project Objectives
High Priority Objectives
Create a bioreactor vessel that thaws cells and stirs the bioreactor to separate the dead and live cells within the constraints of incubator
Second Priority Objectives
Develop a dispensing system that loads a specific amount of cells into each encapsulation device
Other Constraints and Issues
Aseptic and biocompatible
Scalable and re-programmable
WOW Design Solution
Achieve the high and secondary objectives with high precision, especially the system used to count the cells as they load into the encapsulation device
Risk Reduction Strategy
Cells clumping together
Variable velocity and radius for the stirring stage
Contamination of media
Sealed disposable environment with prior sterilization
Inability to separate cells
Varying wait time after stopping stirring
Long term reliability in a high humidity environment
Corrosion resistant material
Fluid level maintenance
Maintain fluid levels with stepper motor controlled pump
Long term reliability of control system
Error Alarms (Watch dog timer)
Intermediate Milestones
Week 6-7 - Have prototype completed and demonstrated to sponsor