Purpose:
The purpose of the Yeast CO2 Optimization lab was to learn how to properly research a formula and demonstrate it in the lab take notes and improve. We learned how to document our findings in the middle of the lab to make more accurate hypothesizes later.
Hypothesis:
I hypothesise that adding baking soda will cause the reaction to be larger and longer as long as I keep the yeast at the right temperature and use proper proportions.
Materials:
- Yeast
- Sugar
- Water
- Baking soda
- Pneumatic trough
- Graduated cylinder
- Hose
- Erlenmeyer flask
- Weigh boat
- Scale
Procedure:
Prepare the pneumatic trough and the arm flask and a hose
Connect the hose to the arm of the flask and the opening to the trough
Fill pneumatic trough with water
Fill a graduated cylinder with water and place upside down in the trough (IMPORTANT: THERE MUST BE NO GAS IN THE CYLINDER)
Add 200 ML of water to a flask on a hot plate
Start heating up to 135F
Add 50g of sugar and fully stir it into the water
Mix in 0.15g of baking soda full stirred in
Add 0.5g of yeast put the cap on and swirl the flask
Wait for reaction to commence for 10 minutes (Set a timer)
End reaction at or around the 10 minute mark
Add 110 ML of water to a flask on a hot plate
Start heating up to 106F
Add 12.25g of sugar and fully stir it into the water
Mix in 0.16g of baking soda full stirred in
Add 5.01g of yeast put the cap on and swirl the flask
Wait for reaction to commence for 10 minutes (Set a timer)
End reaction at or around the 10 minute mark
Add 10 ML of water to a flask on a hot plate
Start heating up to 135F
Add 1.1g of sugar and fully stir it into the water
Mix in 0.1g of baking soda full stirred in
Add 0.45g of yeast put the cap on and swirl the flask
Wait for reaction to commence for 10 minutes (Set a timer)
End reaction at or around the 10 minute mark
Observations:
It was hard to get the cylinder in the trough without any air bubbles
The set up was pretty rigid
When stirring some sugar and baking soda was lost of the stir stick
Almost nothing was produced
I used such little yeast I think that's why I had almost no yield
Lost some sugar and baking soda on the stir stick
Much MUCH MUCH more yield this time
More yeast worked, I wonder if more sugar would make more
No production at all, not even a little bubble
I think I scaled the amounts down wrong
Lost a little bit in in the transfers from weigh boat to flask
I didn't have to scale it this small I might do a lot more everything than this
Lab Technique:
In this lab we used a pneumatic through to measure the amount of CO2 produced, this was accomplished by using a flask with a reaction in it with a cap hose or side arm with a hose that leads into the hose connecter on the pneumatic trough which leads to a hole underneath in the trough. Just to recap we have the gas that forms in the reaction flask leading through a tube to connector to the trough so when the gas is released it pushes through the water and you have the graduated cylinder on top of the hole gilled with water so you can measure how much gas you produced by how much gas there is where the water was .
Data Collection:
In this lab I reenforced lab techniques to become a safer individual in the lab and I learned new techniques like using a pneumatic trough to measure an output of a gas formed in a reaction. I did research on many diffrent formulas and opinions to find what would work best for me in the classroom and then I successfully created and generated a google sheet to record the data on while experimenting so we could all find out what results worked the best and build off each others successes and failures rather than all starting from nothing. We chose to share data because we didn't have much time for this project and we all want each other to succeed so we created a google sheet to share and work together.
I enjoyed this lab, but I wish I had more time, it was a very rushed process because of the time constraints we were put onto us, which made me take rushed notes and not as many as I wanted to. I do really like how we came together as a class fairly quickly came up with a plan and decided we would all work together and who was doing what. I feel that was very helpful epically for the lab reports. In end I just wish I got more time to work with my experiments to test smaller variables smaller variations.