LC/MS Column and Gradient
Column Used: Thermo Accucore RP C18 150*2.1, 2.6μM
Range of metabolites: 70-1000m/z
Resolution: 70000
Polarity: negative
Collision energy: 28-35eV
Gradient: Solvent C: ACN, Solvent D: water with 0.1% FA
Column Used: Thermo Accucore RP C18 150*2.1, 2.6μM
Range of metabolites: 70-1000m/z
Resolution: 70000
Polarity: negative
Collision energy: 28-35eV
Gradient: Solvent C: ACN, Solvent D: water with 0.1% FA