SDS PAGE

•30µg of protein was loaded  and volume made upto 9µl with AMQ. 3µl of 4X SDS gel loading dye was added to it.

•Denatured by boiling at 95ºC for 5 min. folllowed by a short spin

•Loaded on 12% SDS gel with prestatined marker  (thermoscientific) on one side.

•Run on 30mA with 1X Tris glycin SDS buffer full in the inner chamber till the dye is just about to run out of the gel.