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Protocols
Alkaline Lysis Protocol for Isolating Protein from S. cerevisiae
Amine based in-vivo crosslinking of proteins in E.coli
Common buffers and media in lab
SDS PAGE
Freeze thaw lysis of E.coli
Immunocytochemistry (ICC)
Confocal imaging for - S.cerevisiae
Drop-Dilution Growth Assay - S. cerevisiae
DTNB based acetyl transferase activity assay
E.coli Transformation
Isolation of crude mitochondria from S.cerevisiae
Isolation of mitochondria from S.cerevisiae by differential centrifugation
Isolation of pure mitochondria from S.cerevisiae by sucrose density gradient centrifugation
Mini prep- Plasmid DNA isolation (manual method)
Mitoplasting - S.cerevisiae
Oxygen Consumption Assay
Protein expression and Affinity based Purification
Protocol for enriching mother cells of cerevisiae
Protocol for enriching mother cells of cerevisiae
Protocol for enriching mother cells of cerevisiae
Protocol for enriching mother cells of cerevisiae
Protocol for mating of yeast
RbCa competent cell preparation
RNA isolation from Saccharomyces cerevisiae
RNA isolation from Saccharomyces cerevisiae
Transfection
Western Blot
Whole Mount RNA in-situ Hybridization
Yeast transformation
Resources
LC/MS Column and Gradient
LC/MS Column and Gradient
Metabolite extraction from E.coli
Mini prep- Plasmid DNA isolation (manual method)
KC Lab
Isolation of pure mitochondria from S.cerevisiae by sucrose density gradient centrifugation
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