Intensity Time Trace

The second plot shows the background (integrated over the ROI) subtracted from the total intensity.

This graph is useful mostly when studying time series, where it allows monitoring possible intensity fluctuations throughout the measurement.

Time traces from subsequent analyses are added as new plots to the chart.

1. Use File Timestamp: this checkbox can be used if .set files are provided for each time point in the root folder. If no such files are found, or the checkbox is unchecked, the user-defined Time Step (visible only when the checkbox is unchecked) is used to represent the time trace.

2. Use Intensity Correction File: in some cases (as illustrated above), excitation intensity or other acquisition parameters are modified at different time points during the time series. If these changes are known and saved in an Intensity Correction File (whose path is provided in the input box at the bottom of the panel), it is possible to compute and represent a corrected intensity time trace:

The Analysis>>Define Intensity Corrections menu item provides a simple user interface to create such an Intensity Correction File. See the corresponding manual page for details.

Individual plots can be deleted or the whole graph cleared at once using the graph's right-click context menu. Note that the right-click menu changes depending on which part of the graph is is invoked (plot area, chart legend, plot style, cursor legend, scale legend, etc.).