微生物及其染色法

Q: Agar 做成的培養基於65度就會融化，假如想要培養嗜高溫之菌種怎麼辦?

A:可以National Botanical Research Institute’s phosphate growth medium (NBRIP)

Pikovsakaya’s medium (PVK)

    sucrose calcium phosphate medium (SCP) .......等其他種類之耐高溫培養基替代之。

Q:酵母菌(yeast)再行出芽生殖時會產生芽疤(bud scars) ，試問一隻酵母菌最多可以有幾個芽疤?

A:芽疤數=出芽生殖次數，故此問題等同於:一隻酵母菌最多可行幾次出芽生殖?

According to Mateusz Molon,Daughters of the budding yeast from old mothers have shorter replicative lifespans but not total lifespans. Are DNA damage and rDNA instability the factors that determine longevity?,2018，一隻酵母菌最多可行60-80次出芽生殖。