Oliveira PF

Pedro F. Oliveira, Ph.D.

Assistant Professor
Principal Investigator
pfobox@gmail.com

Short Biography

Pedro Fontes Oliveira was conferred his BSc degree in Biochemistry in 1996, at the University of Porto (UP). In 2004, he was conferred a PhD degree in Biomedical Sciences, at the Instituto de Ciencias Biomédicas Abel Salazar from the University of Porto. In 2005, Pedro F. Oliveira started his post-doctoral fellowship at University of Porto and began working on a new line of research now focused on Reproductive Biology, specifically in Sertoli cell physiology. In 2009, Pedro F. Oliveira was hired as Assistant Researcher under the scope of “Ciência 2008 Program” at the Health Science Research Center, University of Beira Interior (CICS-UBI). In 2015, he became an Affiliated Professor at the Institute of Biomedical Sciences Abel Salazar, University of Porto – ICBAS/UP and Principal Researcher at the Institute of Health Research and Innovation (I3S), the largest national health research center. Presently, he is an Assistant Professor at the Department of Chemistry, University of Aveiro and a researcher at the Laboratório Associado para a Química Verde (LAQV@REQUIMTE) - (Bio)Chemistry & Omics Research Group. His research interests cover hormonal control and metabolic modulation of spermatogenesis. Special attention is given to Sertoli cells hormonal modulation and ionic/water transport, and mechanisms by which endocrine disorders/pathologies result in subfertility and infertility.

Funded projects

PTDC/BBB-BQB/1368/2014: "Aquaporins as molecular partners of CFTR in the Blood-Testis Barrier: a crossroad between human male infertility and cystic fibrosis?" - PI: Pedro F. Oliveira

PTDC/BIM-MET/4712/2014: "Obesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility"- PI: Marco G. Alves

"Intrinsic Disability of Sphincter - Study of differences in collagen type and collagenolysis process of urethral support tissue"- Co-Principal Investigators: Pedro Fontes Oliveira, Bercina Candoso and Mário Sousa. Portuguese Society Of Gynecology.

Ended funded projects

PTDC/QUI-BIQ/121446/2010:“Can Hormonal (De)regulation of Ion Transporters in Human Sertoli Cells be Responsible for Infertility?”, P.I.: Pedro Fontes Oliveira.

PTDC/SAU-TOX/117481/2010: “The impact of Exendin-4 therapy in the molecular mechanisms underlying type 2 diabetes-related long-term brain dysfunction”, P.I.: Ana Isabel Duarte.

PMADSC/FM-UC: “Influence of gender on the molecular mechanisms underlying aging-related changes in diabetic brain: The impact of insulin therapy”, P.I.: Paula I. Moreira.

POCTI/BCI/48376/2002: “Electrophysiological characterisation of ionic channels of the human seminiferous epithellial cells”, P.I.: Augusta Rebelo da Costa

2/2.1/BIA/220/94: “Dynamic study of intracellular ion concentrations measured with ion-sensitive fluorescent probes in combination with the patch-clamp technique”, P.I.: Hugo Gil Ferreira.

PBIC/C/BIA/2050/95: “Biomineralization. Cellular and molecular mechanisms of calcification”, P.I.: Augusta Coimbra.

Fellowships and Prizes

2020 – "SPMR Research Award" from the Portuguese Society of Reproductive Medicine (SPMR).

2018 – Best Poster in Basic Research, "High-fat diets compromise male fertility which is not recovered by diet reversion", the 14th Portuguese Congress of Diabetes, Hotel Tivoli Marina Vilamoura, Vilamoura, Portugal, 9-11 March 2018

2010 – Gulbenkian Foundation grant award for attendance of an international meeting (Joint EUROMAR 2010 and 17th ISMAR Conference, Florence, Italy).

2005 – 3-year Post-Doctoral fellowship from the Portuguese Foundation for Science and Technology (FCT).

2014 – 1-year Post-Doctoral fellowship from the project POCTI/BCI/48376/2002: “Electrophysiological characterisation of ionic channels of the human seminiferous epithellial cells” from the Portuguese Foundation for Science and Technology (FCT).

1998 – 4-year PhD fellowship from the Portuguese Foundation for Science and Technology (FCT).

1996 – 2-year Young Researcher fellowship from Junta Nacional de Investigação Científica e Tecnológica (JNICT).

Proceedings in Peer-reviewed Journals

42. Alves MG, Crisóstomo L, Rato L, Jarak I, Silva BM, Raposo JF, Oliveira PF (2019) Uma dieta rica em gordura altera o metabolismo testicular o que compromete a qualidade reprodutiva. Revista Portuguesa de Diabetes; 14 (1) Supp:47-122 (P137)

41. Crisóstomo L, Rato L, Jarak I, Silva BM, Raposo JF, Oliveira PF, Alves MG (2019) Reversão da dieta rica em gordura para uma dieta normal, no início da idade adulta, previne a síndrome metabólica mas não melhora a qualidade espermática. Revista Portuguesa de Diabetes; 14 (1) Supp:23-26 (CO03)

40. Crisóstomo L, Rato L, Jarak I, Silva BM, Raposo JF, Oliveira PF, Alves MG (2018). High-fat diet compromise male fertility that is not surpassed by reversion to a controlled diet. FEBS Open Bio, 8: P.09-023-Tue. doi:10.1002/2211-5463.12453

39. Crisóstomo L, Rato L, Jarak I, Silva BM, Raposo JF, Oliveira PF, Alves MG (2018) Dietas gordas podem comprometer a fertilidade masculina que não é recuperada pelo regresso a uma dieta controlada. Revista Portuguesa de Diabetes; 13 (1) Supp:47-117 (P122)

38. Monteiro JP, Jesus TT, Crisóstomo L, Sousa M, Barros A, Oliveira PF, Alves MG (2017). mTOR coordinates the response to available energy substrates in human Sertoli cells: A defining point for male fertility? FEBS J. 284 (Suppl. 1): SpT.5.4-002. DOI: 10.1111/febs.14171

37. Meneses MJ, Dias TR, Martins FO, Borges DO, Oliveira PF, Macedo MP, Alves MG (2016) “O Knock-out do gene que codifica a insulisina diminui a qualidade espermática de ratinhos” 7th Workshop in Clinical Biochemistry in Acta Farmacêutica Portuguesa 5(2): pp. 115-120

36. Bernardino RL, Alves MG, Silva J, Barros A, Ferraz L, Sousa M, Sá R, Oliveira PF (2016) "Men with Klinefelter syndrome present altered testicular expression of estrogen receptors". 50th Annual Scientific Meeting of the European Society for Clinical Investigation in European Journal of Clinical Investigation. 46(S1):123. ISSN: 1365-2362

35. Dias TR, Alves MG, Silva J, Barros A, Sousa M, Casal S, Silva BM, Oliveira PF (2016) "Effect of epigallocatechin gallate in human Sertoli cells metabolism and mitochondrial function: implications for spermatogenesis". 50th Annual Scientific Meeting of the European Society for Clinical Investigation in European Journal of Clinical Investigation. 46(S1):122. ISSN: 1365-2362

34. Jesus TT, Oliveira PF, Silva J, Barros A, Ferreira R, Sousa M, Cheng CY, Silva BM, Alves MG (2016) "Mammalian target of rapamycin modulates Sertoli cells metabolism and oxidative profile". 50th Annual Scientific Meeting of the European Society for Clinical Investigation in European Journal of Clinical Investigation. 46(S1):121-2. ISSN: 1365-2362

33. Dias TR, Alves MG, Almeida SP, Silva J, Barros A, Sousa M, Silva BM, Silvestre SM, Oliveira PF (2016) "Modulation of human Sertoli cells metabolism by precursors of androgen synthesis: new insights on the role of dehydroepiandrosterone and 7-oxo-dehydroepiandrosterone". 50th Annual Scientific Meeting of the European Society for Clinical Investigation in European Journal of Clinical Investigation. 46(S1):121. ISSN: 1365-2362

32. AD Martins, Monteiro MP, Sousa M, Barros A, Silva J, Carvalho RA, Silva BM, Oliveira PF, Alves MG (2016) "Glucagon-like peptide-1 stimulates lactate production by human Sertoli cells with possible implication to male reproductive potential". 50th Annual Scientific Meeting of the European Society for Clinical Investigation in European Journal of Clinical Investigation. 46(S1):120. ISSN: 1365-2362

31. Rato L, Alves MG, Dias TR, Cavaco JE, Oliveira, PF (2016) Testicular metabolic reprogramming in neonatal streptozotocin-induced type 2 diabetic rats impairs glucose metabolism and promotes glycogen accumulation. Medicine. (In press)

30. Cavaco JE*, Rato L*, Alves MG, Duarte AI, Santos MS, Moreira PI, Oliveira PF (2016). How does Sertoli cell metabolism responds to the reduced testosterone levels induced by progressive stages of diabetes mellitus. Medicine. (In press)

29. Silva BM, Tomas GD, Dias TR, Martins AD, Rato L, Alves MG, Oliveira PF (2016). Sperm quality of pre-diabetic wistar rats restored after a diet with white tea. Medicine. (In press)

28. Ferreira C, Gonçalves A, Rabaça A, Bernardino RL, Barros A, Oliveira PF, Sousa M, Alves MG, Sá R (2016) Metformin preserves human sperm DNA integrity during short-term incubation. Medicine (in press)

27. Rabaça A, Gonçalves A, Ferreira C, Bernardino RL, Barros, A, Alves MG, Sousa M, Oliveira PF, Sá R (2016) Protective effects of n-acetylcysteine on human sperm DNA during short-term etoposide exposure. Medicine(in press)

26. Jesus TT, Oliveira PF, Silva J, Barros A, Ferreira R, Sousa M, Cheng CY, Silva BM, Alves MG (2016) "Mammalian target of rapamycin is a novel modulator of Sertoli cells glycolytic and oxidative profiles". Medicine (in press)

25. Meneses MJ, Bernardino RL, Sá R, Silva J, Barros A, Sousa M, Silva BM., Oliveira PF, Alves MG (2016) The antidiabetic drug pioglitazone alters human Sertoli cells metabolism by increasing its glycolytic efficiency. Medicine (in press)

24. Martins AD, Morreira AC, Sá R, Monteiro MP, Sousa M, Carvalho RA, Silva BM, Oliveira PF, Alves MG (2016). Leptin modulates human Sertoli cells glucose metabolism: relevance for obesity-induced male infertility. Medicine J. (in press)

23. Meneses MJ, Bernardino RL, Sá R, Silva J, Barros A, Sousa M, Silva BM, Oliveira PF, Alves MG (2016) The antidiabetic drugs Pioglitazone and Metformin alter the metabolic cooperation in the testis.IV Encontro Nacional de Pós-Graduação em Ciências Biológicas in CAPTAR 6 (1): 47-48, ISSN 1647-323X

22. Martins AD, Moreira AC, Sá R, Monteiro MP, Sousa M, Carvalho RA, Silva BM, Oliveira PF, Alves MG (2016) Ação da leptina no metabolismo da glucose das células de Sertoli: impacto na fertilidade masculina. CAPTAR. 6(1): . ISSN: 1647-323X

21. Jesus TT, Bernardino RL, Martins AD, Sá R, Sousa M, Alves MG, Oliveira PF (2016) As aquaporinas-4 e -9 são expressas em células de Sertoli de rato e interagem com o CFTR. CAPTAR. 6(1):38-9. ISSN: 1647-323X

20. Dias TR, Alves MG, Bernardino RL, Martins AD, Moreira AC, Silva J, Barros A, Sousa M, Silva BM, Oliveira PF (2016) Caffeine and male fertility: Metabolic effects and oxidative profile in human Sertoli cells. IV Encontro Nacional de Pós-Graduação em Ciências Biológicas in CAPTAR 6 (1): 28-29, ISSN: 1647-323X.

19. Bernardino RL, Martins AD, Costa AR, Silva J, Barros A, Sousa M, Alves MG, Oliveira PF (2016) Influência do estradiol nos transportadores membranares de bicarbonato dependentes de sódio da família SLC4 em células de Sertoli humanas. IV Encontro Nacional de Pós-Graduação em Ciências Biológicas in CAPTAR. 6 (1): 4-6, ISSN 1647-323X

18. Alves MG, Bernardino RL, Martins AD., Jesus TT, Sousa M, Sá R, Oliveira PF (2015) Estrogenic regulation of bicarbonate transporters from SLC4 family in rat Sertoli cells. Human Reproduction. 30(Suppl. 1):161.

17. Martins AD, Sá R, Monteiro MP, Barros A, Silva J, Sousa M, Carvalho RA, Silva BM, Oliveira PF, Alves MG (2015). Ghrelin modulates human Sertoli cells metabolism: relevance for male fertility. FEBS J. 282 (Suppl. 1) 55: 188.

16. Bernardino RL, Martins AD, Costa AR, Silva J, Barros A, Sá R, Sousa M, Alves MG, Oliveira PF (2015). Estrogenic regulation of Na+-dependent bicarbonate transporters from SLC4 family in human Sertoli Cells. FEBS J. 282 (Suppl. 1) 55: 199.

15. Dias TR, Alves MG, Bernardino RL, Martins AD, Moreira AC, Silva J, Barros A, M. Sousa, Silva BM, Oliveira PF (2015). Caffeine-induced alterations in human Sertoli cells metabolism and oxidative profile. FEBS J. 282 (Suppl. 1) 55: 386.

14. Rato L, Duarte AI, Tomás GD, Santos MS, Moreira PI, Socorro S, Cavaco JE, Alves MG, Oliveira PF (2014). Pre-diabetes disrupts testicular PGC1-α/SIRT3 axis prompting oxidative stress. Biochimica et Biophysica Acta (BBA)-Bioenergetics 1837:e64.

13. Vaz CV, Alves MG, Marques R, Moreira PI, Oliveira PF, Cavaco JE, Maia CJ, Socorro S (2014) Androgens regulate gene expression of glucose transporters and glycolytic enzymes in prostate cancer cells. Endocrine Abstracts 35: P571. (DOI: 10.1530/endoabs.35.P571).

12. Correia S, Alves MR, Martins AD, Cavaco JE, Oliveira PF, Socorro S (2014) Effect of 17b-estradiol on germ cell apoptosis by modulating the expression of SCF/c-kit, FasL/FasR and regucalcin. Endocrine Abstracts 35: P702. (DOI: 10.1530/endoabs.35.P702).

11. Cavaco JE, Rato L, Alves MG, Dias TR, Lopes G, Socorro S, Oliveira PF (2013) Fast-Food may Induce Pre-Diabetes Impairing Testicular Function in Rats. Hum Rep. 28: 23-24.

10. Rato L, Duarte AI, Alves MG, Santos MS, Moreira PI, Cavaco JE, Socorro S, Oliveira PF (2013) Testicular mitochondrial bioenergetics is altered in pre-diabetes induced by a high-energy diet in rats. FEBS Journal. 280(S1): 1–661.

9. Alves MG*, Jesus TT*, Bernardino RL, Martins AD, Sá R, Sousa M, Oliveira PF (2013) Water transport in seminiferous tubule: aquaporins identification in Sertoli cells. Proc. 37th IUPS, LB91.

8. Bernardino RL, Martins AD, Jesus TT, Sá R, Sousa M, Socorro S, Alves MG, Oliveira PF (2013) Regulation by estrogens of bicarbonate secretion mechanisms in Sertoli cell. Proc. 37th IUPS, LB113.

7. Martins AD, Simões VL, Alves MG, Cavaco JE, Socorro S, Oliveira PF (2012) Hormonal Regulation of Na+H+ Exchanger 3 (NHE3) in Sertoli Cells: A Possible Role for Estrogens in Spermatogenesis. J. Reproduktionsmed. Endokrinol. 9(5): 371.

6. Alves MG, Socorro S, Silva J, Barros A, Sousa M, Cavaco JE, Oliveira PF (2012) Human Sertoli cells Produce High Amounts of Acetate that is Under Strict Hormonal Control. J. Reproduktionsmed. Endokrinol. 9(5): 370.

5. Alves MG, Gartner A, Pereira T, Amorim I, Carvalho RA, Socorro S, Oliveira PF, Maurício AC (2011). Evidence of metabolic switch during human mesenchymal stem cells differentiation in neural-like cells. European Journal of Clinical Investigation 41:9.

4. Oliveira PF, Moura T, Barros A, Sousa M, Rebelo da Costa A (2007) Effect of specific inhibitors on pHi recovery of Sertoli cells.FEBS J 274 (Suppl. 1): 122.

3. Barrias C, Oliveira PF, Rebelo da Costa A (2000). Ionic transport in the terminal portion of the intestine of Anodonta cygnea. Comp. Biochem. Physiol. 126A (1): 11.

2. Barrias C, Oliveira PF, Maurício AC, Rebelo da Costa AM (1999). Calcium channels of Outer Mantle Epithelium of Anodonta cygnea. Journal of Bioscience 24 (1): 130.

1. Oliveira PF, Lopes IA, Barrias C, Rebelo da Costa AM (1999). The proton pump of the outer mantle epithelium of Anodonta cygnea. Journal of Bioscience 24 (1): 133.

Books

3. Alves MG, Oliveira PF (2018) Sertoli Cells - Methods and Protocols, vol 1748. Methods in Molecular Biology, 1 edn. Humana Press. ISBN: 978-1-4939-7698-0

2. Alves MG, Oliveira PF (2017) Biochemistry of Andrology. Andrology: Current and Future Developments. Bentham Science Publishers, Sharjah, UAE. ISBN: 978-1-68108-501-2

1. Oliveira PF, Alves MG (2016) Sertoli Cell Metabolism and Spermatogenesis. SpringerBriefs in Cell Biology, ed. M. Owen. Springer, United Kingdom, ISBN: 978-3-319-19790-6

Book chapters

19. Pereira SC, Moreira MV, Silva BM, Oliveira PF, Alves MG. Roles of Oxidative Stress in the Male Reproductive System: Potential of Antioxidant Supplementation for Infertility Treatment. Adv Exp Med Biol. 2022;1391:259-274. doi: 10.1007/978-3-031-12966-7_15. PMID: 36472827.

18. Carrageta D.F., Oliveira P.F., Monteiro M.P., Alves M.G. (2020) Adipocyte Specific Signaling. In: Silva J., Freitas M., Fardilha M. (eds) Tissue-Specific Cell Signaling. Springer, Cham. https://doi.org/10.1007/978-3-030-44436-5_15

17. Carrageta DF, Bernardino RL, Alves MG, Oliveira PF (2020) CFTR regulation of aquaporin-mediated water transport. Vitamins and Hormones. 112:163-177. doi: 10.1016/bs.vh.2019.08.004

16. Rato L, Oliveira P. F, Sousa M., Silva B. M. and Alves M. G. (2018). Role of reactive oxygen species in diabetes-induced male reproductive dysfunction. In Samanta L., Henkel R. Agarwal A. Editors. Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction. ISBN: 9780128125014. In Press

15. Dias TR, Alves MG, Silva BM, Oliveira PF (2018) “Nutritional factors and male reproduction” In “Reference Module in Biomedical Sciences", Elsevier (DOI:10.1016/B978-0-12-801238-3.64616-0).

14. Bernardino RL, Alves MG, Oliveira PF (2018) Establishment of Primary Culture of Sertoli Cells. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 1-8. DOI:10.1007/978-1-4939-7698-0

13. Bernardino RL, Alves MG, Oliveira PF (2018) Evaluation of the Purity of Sertoli Cell Primary Cultures. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 9-16. DOI:10.1007/978-1-4939-7698-0

12. Crisóstomo L, Alves MG, Gorga A, Sousa M, Riera MF, Galardo MN, Meroni SB, Oliveira PF (2018) Molecular mechanisms and signalling pathways involved in the nutritional support of spermatogenesis by Sertoli cells. In: Alves MG, Oliveira PF (eds) Sertoli cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 129-155. DOI:10.1007/978-1-4939-7698-0

11. Jarak I, Oliveira PF, Rindone G, Carvalho RA, Galardo MN, Riera MF, Meroni SB, Alves MG (2018) Assessing Sertoli Cell Metabolic Activity. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 157-171. DOI:10.1007/978-1-4939-7698-0

10. Maggio A, Bernardino RL, Gena P, Alves MG, Oliveira PF, Calamita G (2018) A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 279-286. doi:10.1007/978-1-4939-7698-0

9. Dias TR, Carrageta DF, Alves MG, Oliveira PF, Silva BM. (2017) White tea extract. In Nonvitamin and Nonmineral Nutritional Supplements. Editors: S.N. Nabavi & A.S. Silva - Elsevier: Amsterdam, Holland (in press).

8. Nunes AR, Alves MG, Oliveira PF, Silva LR, Silva BM. Bioactive compounds and health-promoting properties of Ficus carica (L.): a review. In Natural Bioactive Compounds from Fruits and Vegetables as Health Promoters (vol. 1). Editors: L.R. Silva & B.M. Silva - Bentham Science Publishers Publishers: Berlin, Germany (in press).

7. Silva BM, Tomás GD, Nunes AR, Ramalhosa E, Pereira JA, Alves MG, Oliveira PF. Phytochemical, nutritional, antioxidant and anticancer properties of Juglans regia (L.) In Natural Bioactive Compounds from Fruits and Vegetables as Health Promoters (vol. 2). Editors: por L.R. Silva & B.M. Silva - Bentham Science Publishers Publishers: Berlin, Germany (in press).

6. Meneses MJ, Bernardino RL, Sousa M, Silva BM, Sá R, Oliveira PF, Alves MG (2015). Regulation of Testicular Glucose Metabolism in (Pre)Diabetes and its Implications to Male Reproductive Health. In Advances in Medicine and Biology. Volume 89. Nova Science Publishers, Inc, New York, USA, ISBN: 978-1-63483-478-0.

5. Nunes AR, Alves MG, Moreira PI, Oliveira PF, Silva BM (2015). Impact of Green Tea (Camellia sinensis L.) Consumption in Diabetes Mellitus-Induced Neurodegeneration. In Green Tea and Health: Antioxidant Properties, Consumption and Role in Disease Prevention. Nova Science Publishers, Inc, New York, USA, ISBN: 978-1-63482-904-5.

4. Alves MG, Rato L, Moreira AC, Oliveira PF. A Célula de Sertoli: Fisiologia, Estrutura e Função. (2015) In Reprodução Humana Masculina: Princípios Fundamentais, ARC Publishing e Editores,ISBN-10: 9899859931.

3. Dias TR, Alves MG, Neuhaus-Oliveira A, Socorro S, Silva BM, Oliveira PF. Implications of Diabetes on sperm glucose uptake and metabolism. In Glucose Uptake: Regulation, Signaling Pathways and Health Implications, Nova Science Publishers, Inc, New York, USA. ISBN: 978-1-62618-671-2.

2. Vaz CV, Alves MG, Marques R, Oliveira PF, Maia CJ, Socorro S. Glucose uptake and androgen responsiveness of prostate cancer cells. In Glucose Uptake: Regulation, Signaling Pathways and Health Implications, Nova Science Publishers, Inc, New York, USA. ISBN: 978-1-62618-671-2.

1. Rato L, Alves MG, Socorro S, Cavaco JE, Oliveira PF. Blood testis barrier: how does the seminiferous epithelium feed the developing germ cells? In Endothelium and Epithelium: Composition, Functions and Pathology, Nova Science Publishers, Inc, New York, USA. ISBN: 978-1-61470-874-2.

Publications

212. Carrageta DF, Pereira SC, Ferreira R, Monteiro MP, Oliveira PF, Alves MG. Signatures of metabolic diseases on spermatogenesis and testicular metabolism. Nat Rev Urol, 2024. https://doi.org/10.1038/s41585-024-00866-y

211. Priscila R. Nunes, Pedro F. Oliveira, Irene Rebelo, Valeria C. Sandrim, Marco G. Alves, Relevance of real-time analyzers to determine mitochondrial quality in endothelial cells and oxidative stress in preeclampsia, Vascular Pharmacology, Volume 155, 2024, 107372. https://doi.org/10.1016/j.vph.2024.107372.

210. Guerra-Carvalho B, Jarak I, Carvalho RA, Pinto S, Correia SC, Barros A, Alves MG, Oliveira PF. Metabolomics of Human Seminal Fluid Reveals Aging-Related Increase in the Amino Acid Content. Journal of Biological Regulators and Homeostatic Agents. 2024, 38(4): 2945-2958 https://doi.org/10.23812/j.biol.regul.homeost.agents.20243804.230

209. Moreira R, Martins AD, Ferreira R, Alves MG, Pereira MdL, Oliveira PF. Impact of Chromium Picolinate on Leydig Cell Steroidogenesis and Antioxidant Balance Using an In Vitro Insulin Resistance Model. Antioxidants. 2024; 13(1):40. https://doi.org/10.3390/antiox13010040

208. Pinto S, Guerra-Carvalho B, Crisóstomo L, Rocha A, Barros A, Alves MG, Oliveira PF. Metabolomics Integration in Assisted Reproductive Technologies for Enhanced Embryo Selection beyond Morphokinetic Analysis. International Journal of Molecular Sciences. 2024; 25(1):491. https://doi.org/10.3390/ijms25010491

207. Madama, D.; Carrageta, D.F.; Guerra-Carvalho, B.; Botelho, M.F.; Oliveira, P.F.; Cordeiro, C.R.; Alves, M.G.; Abrantes, A.M. Impact of Different Treatment Regimens and Timeframes in the Plasmatic Metabolic Profiling of Patients with Lung Adenocarcinoma. Metabolites 2023, 13, 1180. https://doi.org/10.3390/metabo13121180

206. Santos-Pereira, M.; Pereira, S.C.; Rebelo, I.; Spadella, M.A.; Oliveira, P.F.; Alves, M.G. Decoding the Influence of Obesity on Prostate Cancer and Its Transgenerational Impact. Nutrients 2023, 15, 4858. https://doi.org/10.3390/nu15234858

205. Carrageta DF, Freire-Brito L, Guerra-Carvalho B, Bernardino RL, Monteiro BS, Barros A., Oliveira PF, Monteiro MP, Alves MG. (2023). Mitochondrial uncoupling proteins regulate the metabolic function of human Sertoli cells. Reproduction (published online ahead of print 2023), REP-23-0229. Retrieved Dec 11, 2023, from https://doi.org/10.1530/REP-23-0229

204. Crisóstomo L, Oliveira PF, Alves MG. A systematic scientometric review of paternal inheritance of acquired metabolic traits. BMC Biol 21, 255 (2023). https://doi.org/10.1186/s12915-023-01744-6

203. Martins AD, Ribeiro JC, Ferreira R, Alves MG, Oliveira PF. Understanding the age-related alterations in the testis-specific proteome, Expert Review of Proteomics, 2023 DOI: 10.1080/14789450.2023.2274857

202. Costa J, Braga PC, Rebelo I, Oliveira PF, Alves MG. Mitochondria Quality Control and Male Fertility. Biology 12,6. DOI: 10.3390/biology12060827

201. Mateus FG, Moreira S, Martins AD, Oliveira PF, Alves MG, Pereira MdL. L-Carnitine and Male Fertility: Is Supplementation Beneficial? Journal of Clinical Medicine. 2023; 12(18):5796. https://doi.org/10.3390/jcm12185796

200. Braga PC, Bernardino RL, Guerra-Carvalho B, Carrageta DF, Oliveira PF, Rodrigues AS, Alves MG. The progression from mild to severe hyperglycemia coupled with insulin resistance causes mitochondrial dysfunction and alters the metabolic secretome of epithelial kidney cells. Experimental Cell Research. 2023. 113744. https://doi.org/10.1016/j.yexcr.2023.113744

199. Ribeiro JC, Bernardino RL, Gonçalves A, Barros A, Calamita G, Alves MG, Oliveira PF. Aquaporin-7-Mediated Glycerol Permeability Is Linked to Human Sperm Motility in Asthenozoospermia and during Sperm Capacitation. Cells. 2023; 12(15):2003. https://doi.org/10.3390/cells12152003

198. Moreira R, Martins AD, Alves MG, de Lourdes Pereira M, Oliveira PF. A Comprehensive Review of the Impact of Chromium Picolinate on Testicular Steroidogenesis and Antioxidant Balance. Antioxidants. 2023; 12(8):1572. https://doi.org/10.3390/antiox12081572

197. Morais T, Seabra AL, Patrício BG, Carrageta DF, Guimarães M, Nora M, Oliveira PF, Alves MG, Monteiro MP. Dysglycemia Shapes Visceral Adipose Tissue’s Response to GIP, GLP-1 and Glucagon in Individuals with Obesity. Metabolites. 2023; 13(5):587. https://doi.org/10.3390/metabo13050587

196. Carrageta DF, Freire-Brito L, Guerra-Carvalho B, Ribeiro JC, Monteiro BS, Barros A, Oliveira PF, Monteiro MP, Alves MG. Inhibition of Mitochondrial Uncoupling Proteins Arrests Human Spermatozoa Motility without Compromising Viability. Antioxidants. 2023; 12(2):409. https://doi.org/10.3390/antiox12020409

195. Nunes DC, Ribeiro JC, Alves MG, Oliveira PF, Bernardino RL. Male Sex Hormones, Metabolic Syndrome, and Aquaporins: A Triad of Players in Male (in)Fertility. International Journal of Molecular Sciences. 2023; 24(3):1960. https://doi.org/10.3390/ijms24031960

194. Ferreira AF, Santiago J, Silva JV, Oliveira PF, Fardilha M. PP1, PP2A and PP2B Interplay in the Regulation of Sperm Motility: Lessons from Protein Phosphatase Inhibitors. Int J Mol Sci. 2022 Dec 3;23(23):15235. doi: 10.3390/ijms232315235

193. Alves AP, Rocha SM, Mamede AC, Braga PC, Alves MG, Oliveira PF, Botelho FM, Maia CJ. Differential response of hepatocellular carcinoma glycolytic metabolism and oxidative stress markers after exposure to human amniotic membrane proteins. Mol Biol Rep. 2022 Aug;49(8):7731-7741. doi: 10.1007/s11033-022-07598-5

192. Ribeiro JC, Bernardino RL, Carrageta DF, Soveral G, Calamita G, Alves MG, Oliveira PF. CFTR modulates aquaporin-mediated glycerol permeability in mouse Sertoli cells. Cell Mol Life Sci. 2022 Nov 15;79(12):592. doi: 10.1007/s00018-022-04619-1. PMID: 36378343.

191. Boussada, M., Hammami, I., Ben Ali, R., Ammar, A. B., Alves, M., Oliveira, P. F., Akacha, A. B., Abdelkarim, I. L., Zekri, S., & El May, M. V. In vivo exposure to a new 2-cyano-2-p-nitrophenyl-N-benzylthioamide decreases doxorubicin-triggered structural damages in the mature testis. 2022. Andrologia, e14634. doi:10.1111/and.14634

190. Martin-Hidalgo D, González-Fernández L, Bragado MJ, Garcia-Marin LJ, Alves MG, Oliveira PF, The sirtuin 1 activator YK 3-237 stimulates capacitation-related events in human spermatozoa, Reproductive BioMedicine Online, 2022, ISSN 1472-6483, doi:10.1016/j.rbmo.2022.07.011.190. Correia AS, Pereira SC, Morais T, Martins AD, Monteiro MP, Alves MG, Oliveira PF. Obesity-Related Genes Expression in Testes and Sperm Parameters Respond to GLP-1 and Caloric Restriction. Biomedicines. 2022 Oct 17;10(10):2609. doi: 10.3390/biomedicines10102609

189. Silva JV, Santiago J, Matos B, Henriques MC, Patrício D, Martins AD, Duarte JA, Ferreira R, Alves MG, Oliveira P, Oliveira PF, Fardilha M. Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats' Testicular Function. Int J Mol Sci. 2022 Oct 1;23(19):11619. doi: 10.3390/ijms231911619

188. Silva R, Carrageta DF, Alves MG, Silva BM, Oliveira PF. Antioxidants and Male Infertility. Antioxidants 2022, 11, 1152. DOI: 10.3390/antiox11061152

187. Moreira S*, Silva R*, Carrageta DF*, Alves MG, Seco-Rovira V, Oliveira PF, Pereira ML. Carbamate Pesticides: Shedding Light on Their Impact on the Male Reproductive System. Int. J. Mol. Sci. 2022, 23(15), 8206. DOI: 10.3390/ijms23158206

*These authors contributed equally to this work.

186. Silva R, Carrageta DF, Alves MG, Oliveira PF. Testicular Glycogen Metabolism: An Overlooked Source of Energy for Spermatogenesis? BioChem. 2022, 2, 198-214. DOI: 10.3390/biochem2030014

185. Carrageta DF, Freire-Brito L, Oliveira PF, Alves MG. Evaluation of human spermatozoa mitochondrial membrane potential using the JC-1 dye. Current Protocols, 2, e531. DOI: 10.1002/cpz1.531

184. Silva AM, Ribeiro CT, Bernardino RL, Jarak I, Carvalho RA, Pereira-Sampaio MA, de Souza DB, Alves MG, Oliveira PF. Stress Hormone Corticosterone Controls Metabolic Mitochondrial Performance and Inflammatory Signaling of In Vitro Cultured Sertoli Cells. Biomedicines. 2022 Sep 19;10(9):2331. doi: 10.3390/biomedicines10092331

183. Guerra-Carvalho B, Alves MG, Oliveira PF (2022) Obesity and Male Infertility: An Overview. Int J Diabetol Vasc Dis Res. 2022;8(1e):1-3. DOI: 10.19070/2328-353X

182. Ribeiro JC, Martins AD, Jarak I, Carvalho RA, Alves MG, Oliveira PF (2022) Exenatide and Dapagliflozin Combination Enhances Sertoli Cell Secretion of Key Metabolites for Spermatogenesis. Biomedicines, 10, 1115. doi: 10.3390/biomedicines10051115

181. Crisóstomo L, Oliveira PF, Alves MG (2022). Antioxidants, Oxidative Stress, and Non-Communicable Diseases. Antioxidants, 11(6):1080. doi:10.3390/antiox11061080

180. Carrageta DF*, Guerra-Carvalho B*, Spadella MA, Yeste M, Oliveira PF, Alves MG (2022) Animal models of male reproductive ageing to study testosterone production and spermatogenesis. Rev Endocr Metab Disord 2022. DOI: 10.1007/s11154-022-09726-9

179. Braga, PC, Alves, MG, Rodrigues, AS, Oliveira, PF (2022). Mitochondrial Pathophysiology on Chronic Kidney Disease. Int. J. Mol. Sci. 23, 1776. doi:10.3390/ijms23031776

178. Pereira SC, Esperança IF, Pinto S, Barros A, Sousa M, Alves MG, Oliveira PF (2022) Pregnancy Achievement by Medical Assisted Reproduction Is Correlated to the G Protein-Coupled Receptor 30 mRNA Abundance in Human Spermatozoa. Appl. Sci. 2022, 12, 3240. DOI: 10.3390/app12073240

177. Moreira MV, Pereira SC, Guerra-Carvalho B, Carrageta DF, Pinto S, Barros A, Silva BM, Oliveira PF, Alves MG (2022) Hyperoside Supplementation in Preservation Media Surpasses Vitamin C Protection Against Oxidative Stress-induced Damages in Human Spermatozoa. Cell Physiol Biochem 2022;56(S1):1-23. DOI: 10.33594/000000487

176. Ribeiro JC, Carrageta DF, Bernardino RL, Alves MG, Oliveira PF (2022) Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges. Animals 12(3):359. DOI: 10.3390/ani12030359

175. Pereira PR, Carrageta DF, Oliveira PF, Rodrigues A, Alves MG, Monteiro MP (2022) Metabolomics as a tool for the early diagnosis and prognosis of diabetic kidney disease. Med Res Rev. 2022; 1-27. doi:10.1002/med.21883

174. Crisóstomo L, Videira RA, Jarak I, Starčević K, Mašek T, Rato L, Raposo JF, Batterham RL, Oliveira PF, Alves MG (2022) Inherited Metabolic Memory of High-fat Diet Impairs Testicular Fatty Acid Content and Sperm Parameters. Molecular Nutrition & Food Research. doi:10.1002/mnfr.202100680

173. Monteiro BS*, Freire-Brito L*, Carrageta DF, Oliveira PF, Alves MG (2021). Mitochondrial Uncoupling Proteins (UCPs) as Key Modulators of ROS Homeostasis: A Crosstalk between Diabesity and Male Infertility? Antioxidants, 10(11), 1746. doi:10.3390/antiox10111746

172. Pereira SC, Oliveira PF, Oliveira SR, Pereira ML, Alves MG (2021). Impact of Environmental and Lifestyle Use of Chromium on Male Fertility: Focus on Antioxidant Activity and Oxidative Stress. Antioxidants, 10(9), 1365. doi:10.3390/antiox10091365

171. Ribeiro JC, Braga PC, Martins AD, Silva BM, Alves MG, Oliveira PF (2021). Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility. Antioxidants, 10(9), 1441. doi:10.3390/antiox10091441

170. Ribeiro JC, Nogueira-Ferreira R, Amado F, Alves MG, Ferreira R, Oliveira, PF (2021). Exploring the role of oxidative stress in sperm motility: a proteomics network approach. Antioxidants & Redox Signaling, 0(ja), null. doi:10.1089/ars.2021.0241

169. Guerra-Carvalho B*, Carrageta DF*, Crisóstomo L*, Carvalho RA, Alves MG, Oliveira PF (2021). Molecular mechanisms regulating spermatogenesis in vertebrates: Environmental, metabolic, and epigenetic factor effects. Animal reproduction science, 106896. doi:10.1016/j.anireprosci.2021.106896

168. Crisóstomo L, Jarak I, Rato L, Raposo JF, Batterham RL, Oliveira PF, Alves MG (2021). Inheritable testicular metabolic memory of high-fat diet causes transgenerational sperm defects in mice. Sci Rep, 11(1), 9444. doi:10.1038/s41598-021-88981-3

167. Pereira SC, Martins AD, Monteiro MP, Pinto S, Barros A, Oliveira PF, Alves MG (2021). Expression of obesity-related genes in human spermatozoa affects the outcomes of reproductive treatments. F&S Science. doi:10.1016/j.xfss.2021.03.004

166. Ribeiro JC, Alves MG, Amado F, Ferreira R, Oliveira PF (2021) Insights and clinical potential of proteomics in understanding spermatogenesis. Expert Reviews of Proteomics. doi: 10.1080/14789450.2021.1889373

165. Agarwal A, Leisegang K, Panner Selvam MK, Durairajanayagam D, Barbarosie C, Finelli R, Sengupta P, Dutta S, Majzoub A, Pushparaj PN, Elbardisi H, Sharma R, Gupta S, Arafa M, Roychoudhury S, Alves MG, Oliveira PF, Henkel R (2021) An online educational model in andrology for student training in the art of scientific writing in the COVID-19 pandemic. Andrologia. e13961. doi: 10.1111/and.13961

164. Agarwal A, Leisegang K, Majzoub A, Henkel R, Finelli R, Panner Selvam MK, Tadros N, Parekh N, Ko EY, Cho CL, Arafa M, Alves MG, Oliveira PF, Alvarez JG, Shah R (2021) Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence. World Journal of Men's Health. doi: 10.5534/wjmh.200196

163. Correia SC, Machado NJ, Alves MG, Oliveira PF, Moreira PI (2021) Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenergetic Profile in the Triple Transgenic Mouse Model of Alzheimer's Disease. International Journal of Molecular Sciences. 22(1):461. doi: 10.3390/ijms22010461

162. Crisóstomo L, Oliveira PF, Alves MG (2021) Sperm, metabolic memory, and echoes from Lamarck. European Journal of Clinical Investigation. doi:10.1111/eci.13492

161. Pereira SC, Martins AC, Moreira BP, Bernardino RL, Barros A, Monteiro MP, Oliveira PF, Alves MG (2021) Obesity-related genes are expressed in human Sertoli cells and modulated by energy homeostasis regulating hormones. Journal of Cellular Physiology, . doi:10.1002/jcp.30230

160. Ribeiro J C, Alves MG, Yeste M, Cho YS, Calamita G, Oliveira PF (2021). Aquaporins and (in)fertility: More than just water transport. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1867(3), 166039. doi:10.1016/j.bbadis.2020.166039

159. Pereira SC*, Crisóstomo L*, Sousa M, Oliveira PF, Alves MG (2020) Metabolic diseases affect male reproduction and induce signatures in gametes that may compromise the offspring health. Environmental Epigenetics, 6. doi:10.1093/eep/dvaa019

158. Crisóstomo L, Videira RA, Jarak I, Starčević K, Mašek T, Rato LP, Raposo JF, Batterham RL, Oliveira PF, Alves MG (2020) Diet during early life defines testicular lipid content and sperm quality in adulthood. American Journal of Physiology-Endocrinology & Metabolism. 319: E1061–E1073. doi:10.1152/ajpendo.00235.2020

157. Moreira BP, Silva AM, Martins AD, Monteiro MP, Sousa M, Oliveira PF, Alves MG (2020) Effect of Leptin in Human Sertoli Cells Mitochondrial Physiology. Reproductive Sciences, 10.1007/s43032-020-00328-x

156. Mesquita G, Tânia S, Gomes AC, Oliveira PF, Alves MG, Fernandes R, Almeida AA, Moreira AC, Gomes MS. H-Ferritin is essential for macrophages’ capacity to store or detoxify exogenously added iron. Scientific Reports. 2020 Feb; 10:3061. doi: http://dx.doi.org/10.1038/s41598-020-59898-0

155. Gervasi MG, Soler AJ, González-Fernández L, Alves MG, Oliveira PF, Martín-Hidalgo D. Extracellular vesicles, the road toward the improvement of ART outcomes. Animals. 2020 Nov; 10(11):2171. doi: https://doi.org/10.3390/ani10112171

154. Carrageta DF, Bernardino RL, Soveral G, Giuseppe C, Alves MG, Oliveira PF. Aquaporins and male (in)fertility: Expression and role throughout the male reproductive tract. Archives of Biochemistry and Biophysics. 2020 Jan; 679:108222. doi: http://dx.doi.org/10.1016/j.abb.2019.108222

153. Silva JV, Santiago J, Sousa M, Oliveira PF, Fardilha M. New evidences of ubiquitin-proteasome system activity in human sperm. Biochimica et Biophysica Acta. Molecular Cell Research. 2020 Dec;1868(3):118932. doi: https://doi.org/10.1016/j.bbamcr.2020.118932

152. Rabaça A, Ferreira C, Bernardino RL, Alves MG, Oliveira PF, Viana P, Barros A, Sousa M, Sá R. Use of antioxidant could ameliorate the negative impact of etoposide on human sperm DNA during chemotherapy. Reproductive Biomedicine Online. 2020 May;40(20):856-866. doi: https://doi.org/10.1016/j.rbmo.2020.01.029

150. Carrageta DF, Guerra-Carvalho B, Sousa M, Barros A, Oliveira PF, Monteiro MP, Alves MG (2020) Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli. Antioxidants, 9(8), 750. doi: 10.3390/antiox9080750

149. Crisóstomo L, Pereira SC, Monteiro MP, Raposo JF, Oliveira PF, Alves MG (2020) Lifestyle, metabolic disorders and male hypogonadism – A one-way ticket? Molecular and Cellular Endocrinology, 110945. doi: 10.1016/j.mce.2020.110945

148. Pinto S*, Carrageta DF*, Alves MG, Rocha A, Agarwal A, Barros A, Oliveira PF (2020) Sperm selection strategies and their impact on assisted reproductive technology outcomes. Andrologia. e13725. doi:10.1111/and.13725

147. Pereira SS, Jarak I, Carvalho RA, Oliveira PF, Alves MG, Guimarães M, Almeida R, Pereira AM, Wewer Albrechtsen NJ, Holst JJ, Nora M, Monteiro MP (2020) Different Malabsorptive Obesity Surgery Interventions Result in Distinct Postprandial Amino Acid Metabolomic Signatures (2020) Obesity Surgery. doi:10.1007/s11695-020-04774-z

146. Mesquita G, Silva T, Gomes AC, Oliveira PF, Alves MG, Fernandes R, Almeida AA, Moreira AC, Gomes MS (2020) H-Ferritin is essential for macrophages' capacity to store or detoxify exogenously added iron. Scientific Reports;10(1):3061. doi:10.1038/s41598-020-59898-0

145. Dias TR, Martín-Hidalgo D, Silva BM, Oliveira PF, Alves MG (2020). Endogenous and exogenous antioxidants as a tool to ameliorate male infertility induced by reactive oxygen species. Antioxidants & Redox Signaling. doi:10.1089/ars.2019.7977

144. Carrageta DF, Oliveira PF, Alves MG, Monteiro MP (2019) Obesity and male hypogonadism: Tales of a vicious cycle. Obesity Reviews. 20: 1148– 1158. doi: 10.1111/obr.12863

143. Braga PC, Pereira SC, Ribeiro JP, Sousa M, Monteiro MP, Oliveira PF, Alves MG. Late-Onset Hypogonadism and Lifestyle-Related Metabolic Disorders (2020) Andrology. doi:10.1111/andr.12765

142. Silva JF, Moreira BP, Rato L, Pereira ML, Oliveira PF, Alves MG. Is Technical-grade Chlordane an Obesogen? (2020). Current Medicinal Chemistry. doi:10.2174/0929867327666200121122208

141. Jarak I, Pereira SS, Carvalho RA, Oliveira PF, Alves MG, Guimarães M, Albrechtsen NJW, Holst JJ, Nora M, Monteiro MP (2019) Gastric Bypass with Different Biliopancreatic Limb Lengths Results in Similar Post-absorptive Metabolomics Profiles. Obesity Surgery. doi:10.1007/s11695-019-04294-5

140. Carrageta DF, Bernardino RL, Soveral G, Calamita G, Alves MG, Oliveira PF (2019) Aquaporins and male (in)fertility: Expression and role throughout the male reproductive tract. Archives of Biochemistry and Biophysics. doi:10.1016/j.abb.2019.108222

139. Martins AD, Jarak I, Morais T, Carvalho RA, Oliveira PF, Monteiro MP, Alves MG (2019) Caloric restriction alters the hormonal profile and testicular metabolome resulting in alterations of sperm head morphology. American Journal of Physiology - Endocrinology and Metabolism. doi:10.1152/ajpendo.00355.2019

138. Moreira BP, Silva JF, Jarak I, de Lourdes Pereira M, Oliveira PF, Alves MG (2020) Technical-grade chlordane compromises rat Sertoli cells proliferation, viability and metabolic activity. Toxicology in vitro 63: 104673. doi:10.1016/j.tiv.2019.104673

137. Crisóstomo L, Rato L, Jarak I, Silva BM, Raposo JF, Batterham RL, Oliveira PF, Alves MG (2019) A switch from high-fat to normal diet does not restore sperm quality but prevents metabolic syndrome. Reproduction 158(4), 377–387. doi: 10.1530/REP-19-0259

136. Boussada M, Dias TR, Crisóstomo L, Akacha AB, Ali RB, El May MV, Alves MG, Oliveira PF (2019) A new thiocyanoacetamide (2-cyano-2-p-nitrophenyl-N-benzylthioamide) reduces doxorubicin-induced in vitro toxicity in Sertoli cells by decreasing apoptosis and autophagy. Theriogenology 140, 188-200. doi: 10.1016/j.theriogenology.2019.08.030

135. Martins AD, Oliveira PF, Alves MG (2019) Assessment of sertoli cell proliferation by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and sulforhodamine b assays. Current Protocols in Toxicology, 81, e85. doi: 10.1002/cptx.85

134. Borges DO, Meneses MJ, Dias TR, Martins FO, Oliveira PF, Alves MG, Macedo MP (2019) Data on metabolic profile of insulin-degrading enzyme knockout mice. Data in Brief (DOI:10.1016/j.dib.2019.104023)

133. Dias TR, Bernardino RL, Alves MG, Silva J, Barros A, Sousa M, Casal S, Silva BM, Oliveira PF (2019) L-theanine promotes cultured human Sertoli Cells proliferation and modulates glucose metabolism. European Journal of Nutrition (DOI:10.1007/s00394-019-01999-2)

132. Bernardino RL, Carrageta DF, Sousa M, Alves MG, Oliveira PF. (2019) pH and male fertility: making sense on pH homeodynamics throughout the male reproductive tract. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-019-03170-w

131. Bernardino RL, D'Souza WN, Rato L, Rothstein JL, Dias TR, Chui D, Wannberg S, Alves MG, Oliveira PF (2019) Knockout of MCT1 results in total absence of spermatozoa, sex hormones dysregulation, and morphological alterations in the testicular tissue. Cell & Tissue Research. 1-7. doi:10.1007/s00441-019-03028-4

130. Moreira BP, Oliveira PF, Alves MG (2019) Molecular Mechanisms Controlled by mTOR in Male Reproductive System. International Journal of Molecular Sciences. 20(7), 1633. DOI: 10.3390/ijms20071633

129. Meneses MJ, Borges DO, Dias TR, Martins FO, Oliveira PF, Macedo MP, Alves MG (2019) Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality. Mol Cell Endocrinol 486:11-17. doi:10.1016/j.mce.2019.02.011

128. Bernardino RL, Dias TR, Moreira BM, Cunha M, Barros A, Oliveira E, Sousa M, Alves MG, Oliveira PF (2019) Carbonic anhydrases are involved in mitochondrial biogenesis and control the production of lactate by human Sertoli cells. The FEBS Journal. (In press). DOI: 10.1111/febs.14779

127. Moreira BP, Monteiro MP, Sousa M, Oliveira PF, Alves MG (2018) Insights into leptin signaling and male reproductive health: the missing link between overweight and subfertility? Biochemical Journal. 475(22):3535-3560. DOI: 10.1042/BCJ20180631

126. Martins AD, Monteiro MP, Silva BM, Barros A, Sousa M, Carvalho RA, Oliveira PF, Alves MG (2019) Metabolic dynamics of human Sertoli cells are differentially modulated by physiological and pharmacological concentrations of GLP-1. Toxicol Appl Pharmacol. 362:1-8 (DOI: 10.1016/j.taap.2018.10.009)

125. Lopes AC, Oliveira PF, Sousa M (2018) Shedding light into the relevance of telomeres in human reproduction and male factor infertility. Biology of Reproduction (in press)

124. Carrageta DF, Dias TR, Jarak I, Alves MG, Oliveira PF, Van der Walt MM, Terre’Blanche G, Monteiro MP, Silva BM (2018) 8-(3-phenylpropyl)-1,3,7-triethylxanthine is a synthetic caffeine substitute with stronger metabolic modulator activity. Toxicology in Vitro. 53:114-120 3.1 3.2 - Toxicology (Q2). (DOI:10.1016/j.tiv.2018.08.002)

123. Pereira SS, Costa MM, Alves MG, Oliveira PF, Jarak I, Monteiro MP, Pignatelli D. MAPK/ERK pathway inhibition is a promising treatment target for adrenocortical tumors Journal of Cellular Biochemistry. (IN PRESS – Accepted for publication) 3.0 3.1 - Biochemistry, Genetics and Molecular Biology (Q1). (DOI: 10.1002/jcb.27451)

122. Monteiro JM, Alves MG, Oliveira PF, Silva BM. Pharmacological potential of methylxanthines: Retrospective analysis and future expectations. Critical Reviews in Food Science and Nutrition. 6:1-29. 6.0 6.7 - Biochemistry, Genetics and Molecular Biology (Q1) Pharmacology, Toxicology and Pharmaceutics (Q1). (DOI: 10.1080/10408398.2018.1461607)

121. Bernardino RL, Carrageta DF, Silva AM, Calamita G, Alves MG, Soveral G, Oliveira PF (2018) Estrogen Modulates Glycerol Permeability in Sertoli Cells through Downregulation of Aquaporin-9. Cells. 7(10), 153. 4.8 - Biochemistry (Q1). (DOI:10.3390/cells7100153)

120. Silveira AC, Dias JP, Santos VM, Oliveira PF, Alves MG, Rato L, Silva BM (2018) The action of polyphenols in Diabetes Mellitus and Alzheimer's disease: a common agent for overlapping pathologies. Curr Neuropharmacol. (DOI: 10.2174/1570159X16666180803162059)

119. Jarak I, Almeida S, Carvalho RA, Sousa M, Barros A, Alves MG, Oliveira PF (2018) Senescence and declining reproductive potential: Insight into molecular mechanisms through testicular metabolomics. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. (DOI:10.1016/j.bbadis.2018.07.028)

118. Alves A, Mamede AC, Alves MG, Oliveira PF, Rocha SM, Botelho F, Maia CJ (2018) Glycolysis inhibition as a strategy for hepatocellular carcinoma treatment? Curr Cancer Drug Targets. 18:1-1. (DOI:10.2174/1568009618666180430144441)

117. Santiago J, Silva JV, Alves MG, Oliveira PF, Fardilha M. Testicular aging: an overview of ultrastructural, cellular and molecular alterations. The Journals of Gerontology. Doi: 10.1093/gerona/gly082

116. Bernardino RL, Alves MG, Oliveira PF (2018) Establishment of Primary Culture of Sertoli Cells. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 1-8. DOI:10.1007/978-1-4939-7698-0

115. Bernardino RL, Alves MG, Oliveira PF (2018) Evaluation of the Purity of Sertoli Cell Primary Cultures. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 9-16. DOI:10.1007/978-1-4939-7698-0

114. Crisóstomo L, Alves MG, Gorga A, Sousa M, Riera MF, Galardo MN, Meroni SB, Oliveira PF (2018) Molecular mechanisms and signalling pathways involved in the nutritional support of spermatogenesis by Sertoli cells. In: Alves MG, Oliveira PF (eds) Sertoli cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 129-155. DOI:10.1007/978-1-4939-7698-0

113. Jarak I, Oliveira PF, Rindone G, Carvalho RA, Galardo MN, Riera MF, Meroni SB, Alves MG (2018) Assessing Sertoli Cell Metabolic Activity. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 157-171. DOI:10.1007/978-1-4939-7698-0

112. Maggio A, Bernardino RL, Gena P, Alves MG, Oliveira PF, Calamita G (2018) A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells. In: Alves MG, Oliveira PF (eds) Sertoli Cells - Methods and Protocols. Methods in Molecular Biology, vol 1748, 1 edn. Humana Press, pp 279-286. doi:10.1007/978-1-4939-7698-0

111. Carrageta DF, Dias TR, Alves MG, Oliveira PF, Monteiro MP, Silva BM (2018) Anti-obesity potential of natural methylxanthines. Journal of Functional Foods. 43:84-94. DOI: https://doi.org/10.1016/j.jff.2018.02.001

110. Oliveira PF, Crisóstomo L, Sousa M, Monteiro MP, Macedo P, Raposo J, Alves MG (2017). O papel do Espermatozoide na Transmissão à Descendência do Risco para Diabetes Mellitus. [The Role of Sperm in the Transmission to the Offspring of the Risk for Diabetes Mellitus]. Revista Portuguesa de Diabetes. 12(4): 149-158.

109. Maresch CC, Stute DC, Alves MG, Oliveira PF, de Kretser DM, Linn T (2017) Diabetes-induced hyperglycemia impairs male reproductive function: a systematic review. Hum Reprod Update. 24(1):86-105. (DOI: 10.1093/humupd/dmx033)

108. Cardoso AM, Alves MG, Sousa AC, Jarak I, Carvalho RA, Oliveira PF, Cavaco JE, Rato L (2017) The effects of the obesogen tributyltin on the metabolism of Sertoli cells cultured ex vivo. Archives of Toxicology. (DOI:10.1007/s00204-017-2091-x)

107. Crisóstomo L, Alves MG, Calamita G, Sousa M, Oliveira PF (2017) Glycerol and testicular activity: the good, the bad and the ugly. Molecular Human Reproduction. 23(9), 583-655 (DOI: 10.1093/molehr/gax049)

106. González-Fernández L, Sánchez-Calabuig MJ, Alves MG, Oliveira PF. Macedo S, Gutiérrez-Adán A, Rocha A, Macías-García B. Expanded equine cumulus-oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus-oocyte complexes Reproduction, Fertility and Development. (DOI: 10.1071/RD16441)

105. Mathur PP, Alves MG, Oliveira PF (2017) Obesogens and sperm maturation. Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry. (in press)

104. Mathur PP, Rato L, Sousa AC, Oliveira PF, Alves MG (2017) Sperm Maturation as a Possible Target of Obesogens. Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry. (DOI: 10.2174/1871522217666170420092258)

103. Alves MG, Moreira Â, Guimarães M, Nora M, Sousa M, Oliveira PF, Monteiro MP (2017) Body mass index is associated with region-dependent metabolic reprogramming of adipose tissue. BBA Clinical. 8:1-6. (DOI: 10.1016/j.bbacli.2017.05.001)

102. Silva BM, Oliveira PF, Alves MG, Dias TR (2017) Editorial: Bioactive Substances from Medicinal Plants for Metabolic Disorders. Curr Med Chem. 24(4):332-333.(DOI:10.2174/092986732404170302223350)

101. Almeida SP, Rato L, Sousa M, Alves MG, Oliveira PF (2017) Fertility and sperm quality in the aging male. Current Pharmaceutical Design. 23(46): 1-1. (DOI:10.2174/1381612823666170503150313)

100. Dias TR, Alves MG, Silva J, Barros A, Sousa M, Casal S, Silva BM, Oliveira PF (2017) Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile. Toxicology In Vitro. 41:214-222 (DOI: 10.1016/j.tiv.2017.03.006)

99. Oliveira PF, Sousa M, Silva BM, Monteiro MP, Alves MG (2017) Obesity, energy balance and spermatogenesis. Reproduction.153(6):R173-R185. (DOI: 10.1530/REP-17-0018)

98. Crisóstomo L, Sousa M, Alves MG, Oliveira PF F (2017) The Burden of Metabolic Diseases on Male Reproductive Health. Int J Diabetol Vasc Dis Res. 5(1e), 1-2. (DOI:10.19070/2328-353X-1700012e)

97. Jesus TT, Oliveira PF, Sousa M, Cheng CY, Alves MG (2017) Mammalian target of rapamycin (mTOR): a central regulator of male fertility? Critical Reviews in Biochemistry and Molecular Biology. 52(3):235-253. (DOI: 10.1080/10409238.2017.1279120)

96. Oliveira PF, Cheng CY, Alves MG (2017) Emerging Role for Mammalian Target of Rapamycin in Male Fertility. Trends Endocrinology & Metabolism. 28(3):165-167. (DOI:10.1016/j.tem.2016.12.004)

95. Cardoso AM, Alves MG, Mathur PP, Oliveira PF, Cavaco JE, Rato L (2017) Obesogens and Male Fertility. Obesity Reviews. 18(1):109-125.(DOI:10.1111/obr.12469)

94. Dias TR, Alves MG, Casal S, Silva BM, Oliveira PF (2016) Promising potential of dietary (poly)phenolic compounds in the prevention and treatment of diabetes mellitus. Current Medicinal Chemistry. 23:1-18 (DOI: 10.2174/0929867323666160905150419).

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90. Bernardino RL, Marinelli RA, Maggio A, Gena P, Cataldo I, Alves MG, Svelto M, Oliveira PF, Calamita G (2016) Hepatocyte and Sertoli Cell Aquaporins, Recent Advances and Research Trends. Int J Mol Sci. 17(7): 1096 (DOI:10.3390/ijms17071096)

89. Martins AD, Sá R, Monteiro MP, Barros A, Carvalho RA, Silva BM, Oliveira, PF, Alves MG (2016). Ghrelin acts as energy status sensor of male reproduction by modulating Sertoli cells glycolytic metabolism and mitochondrial bioenergetics. Mol. Cell. Endocrinol. 434:199-209 (DOI: 10.1016/j.mce.2016.07.008)

88. Bernardino RL, Alves MG, Silva J, Barros A, Ferraz L, Sousa M, Sá R, Oliveira PF (2016) Expression of estrogen receptorsalpha (ER-α), beta (ER-β) and G protein-coupled receptor 30 (GPR30) in testicular tissue of men with Klinefelter syndrome. Horm Metab Res. 48(06): 413-415. (DOI: 10.1055/s-0042-105151)

87. Rato L, Alves MG, Sousa M, Silva BM, Oliveira PF (2016) Sirtuins: novel players in male reproductive health. Curr. Med. Chem. 23(11):1084-99. (DOI:10.2174/0929867323666160229114248)

86. Dias TR, Bernardino RL, Meneses MJ, Sousa M, Sá R, Alves MG, Silva BM, Oliveira PF (2016) Emerging potential of natural products as an alternative strategy to pharmacological agents used against metabolic disorders” Curr Drug Metab. 17(6): 582-597. (DOI: 10.2174/1389200217666160229113629)

85. Bernardino RL, Costa AR, Martins AD, Silva J, Barros A, Sousa M, Sá R, Alves MG, Oliveira PF (2016) Estradiol modulates Na+ -dependent HCO3 - transporters altering intracellular pH and ion transport in human Sertoli cells: A role on male fertility? Biol Cell. 108(7): 179-188 (DOI: 10.1111/boc.201500094)

84. Dias TR, Alves MG, Casal S, Silva BM, Oliveira PF (2016) Single and synergistic effect of major tea components caffeine, epigallocatechin-3-gallate and L-theanine in rat sperm viability. Food Funct. 7(3):1301-5 (DOI: 10.1039/C5FO01611H)

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82. Jesus TT, Oliveira PF, Silva J, Barros A, Ferreira R, Sousa M, Cheng CY, Silva BM, Alves MG (2016) Mammalian target of rapamycin controls glucose consumption and redox balance in human Sertoli cells. Fertil Steril. 105(3):825–833 (DOI: 10.1016/j.fertnstert.2015.11.032)

81. Alves MG, Martins AD, Jarak I, Barros A, Silva J, Sousa M, Oliveira PF (2016) Testicular lactate content is compromised in men with Klinefelter syndrome. Mol. Reprod. Dev. 83(3):208-216 (DOI: 10.1002/mrd.22608)

80. Rato L*, Meneses MJ*, Silva BM, Sousa M, Alves MG, Oliveira PF (2015) New insights on hormones and factors that modulate Sertoli cell metabolism. Histol Histopathol 31(5):499-513 (DOI: 10.14670/HH-11-717) * both authors contributed equally

79. Rabaça A, Sousa M, Alves MG, Oliveira PF, Sá R (2015) Novel drug therapies for fertility preservation in men undergoing chemotherapy: clinical relevance of protector agents. Curr. Med. Chem. 22(29):3347-3369. (DOI: 10.2174/0929867322666150821094334)