Wet Mount
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What is Wet Mount?
What is Wet Mount?
"Wet Mount" is a technique scientists use to observe moving cells under a microscope to get a clearer image.
Materials Needed:
1. Object of Experiment 6. Beaker with Water
2. Microscope 7. Dropper
3. Glass Slide 8. Knife
4. Cover Slip
5. Forceps
Steps of How to Prepare a Wet Mount Slide using an Onion:
Step 1:
Step 1:
Get a glass slide and cover slip for yourself and make sure they are both thoroughly washed and dried.
Step 2:
Step 2:
Remove a single layer of epidermal cells from the inner (concave) side of the scale leaf (The thinner the better).
Step 3:
Step 3:
Place the single layer of onion cell epithelium on a glass slide. Make sure that you do not fold it over or wrinkle it.
Step 4:
Step 4:
Place 3 drops of water on your onion tissue.
Step 5:
Step 5:
Put the cover slip on the tissue and gently tap out any air bubbles using the forceps.
Step 6:
Step 6:
Observe the cells under 10x with the diaphragm wide open. Slowly reduce the light intensity by closing the diaphragm, and observe the image.
Video:
https://www.youtube.com/watch?v=hAZV_OCSyQ8
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