Lattice light-sheet microscopy (LLSM) is a modified version of light-sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can provide information about dynamic biological processes.
The objective of the work was to trace the chromosomes of C. Elegant embryos, wherein the chromosomes were detected and transformed into lines to facilitate endpoint tracking. This method offered an opportunity to comprehend the arrangement of chromosomes during early-stage cell division.
This study aimed to elucidate the impact of nanoparticles on cytoskeletal changes within cells. The presence of nanoparticles on the cytoskeleton was detected using wavelet filters and subsequently tracked using Jaqaman's algorithm, implemented through Amira. As the cytoskeleton is considered a fundamental structural framework influencing cell shape and motility, the footprint of nanoparticles can serve as a valuable marker in cellular processes.
The objective of this study was to trace the embryonic lineage of C. Elegant. Segmentation of the embryos was achieved through watershed transformation, while the tracing process involved identifying overlapped regions over time. The analysis provided valuable insights into the timing of cell proliferation, as well as the corresponding volumes and positions of the cells.