IPOLπ presents collagen microstructure through color snapshot acquisition, where the orientation-encoded color repeats every 180°. This technique overcomes the major limitation of IPOL, allowing for distinguishing two orthogonal fibers from color. In addition, IPOLπ can distinguish backgrounds and non-birefringent tissues via image brightness. 

10.1016/j.optlaseng.2023.107594 

IPOL visualizes the collagen microstructure in real time that encodes information on collagen fiber orientation and density via a color snapshot image. This technique provides better spatial and temporal resolutions than conventional polarized light microscopy. 

10.1002/jbio.202000326 

This study was to combine a novel polarized light imaging technique with customized stretch devices to characterize the relationship between crimp morphology and local stretch in the poster pole of the eye. The direct measurements of collagen fiber recruitment help drive fiber-based constitutive model formulations for the posterior pole. 

10.1016/j.exer.2022.108967 

10.1101/2023.05.07.539784