PUBLICATIONS

Asterisk: Corresponding author(s)

Green: Graduate student in this lab

<Nagoya City University: 2017-Present>

Takano Y., Ishiwata-Kimata Y., Ushioda R., Kimata Y., and Nakatsukasa K.*

Hydroxyurea modulates thiol–disulfide homeostasis in the yeast endoplasmic reticulum

Life Science Alliance (2025) doi: 10.26508/lsa.202503225, Commentary in Japanese

Nonaka K., Nishimura K.*, Uesaka K., Mishiro-Sato E.,  Fukase M., Kato R., Okumura F., Nakatsukasa K., Obara K.*, and Kamura K.*

Snf1 and yeast GSK3-β activates Tda1 to suppress glucose starvation signaling

EMBO Reports (2025) doi.org/10.1038/s44319-025-00456-y

Nishio K., Mizushima T., and Nakatsukasa K.*

Structural basis for the recognition of citrate synthase by the SCFUcc1 ubiquitin ligase complex.

SPring-8/SACLA Research Frontiers 2023 (2024)

Eiyama A.,  Onishi M., Omi Y., Nagumo S., Nakatsukasa K.,  and Okamoto K.

ER-associated degradation by Doa10 restrains mitophagy in yeast

bioRxiv  (2024) (Posted on April 09, 2024). 

Ikeda T., Yamazaki K., Okumura F., Kamura T.*, and Nakatsukasa K.*

Role of the San1 ubiquitin ligase in the heat stress-induced degradation of non-native Nup1 in the nuclear pore complex

Genetics (2024) Feb 1:iyae017. doi: 10.1093/genetics/iyae017. 

Hayashi M., Kawarasaki T., and Nakatsukasa K.*

Degradation of citrate synthase lacking the mitochondrial targeting sequence is inhibited in cells defective in Hsp70/Hsp40 chaperones under heat stress conditions

FEMS Yeast Research (2023) Dec 21:foad054. doi: 10.1093/femsyr/foad054.

Nishio K.† , Kawarasaki T.† , Sugiura Y.† , Matsumoto S., Konoshima A., Takano Y., Hayashi M., Okumura F., Kamura T.*, Mizushima T.*, and Nakatsukasa K.*

Defective import of mitochondrial metabolic enzyme elicits ectopic metabolic stress

Science Advances (2023) Apr 14;9(15):eadf1956. doi: 10.1126/sciadv.adf1956. Epub 2023 Apr 14., Commentary in Japanese

Takano Y. and Nakatsukasa K.*

Hydroxyurea inhibits ERAD-L independently of S-phase arrest in budding yeast

bioRxiv (2023) (Posted on January 23, 2023) 

Kawarasaki T. and Nakatsukasa K.*

Metabolomics analysis of an AAA-ATPase Cdc48-deficient yeast strain

Heliyon (2023) Jan 24;9(2):e13219. doi: 10.1016/j.heliyon.2023.e13219. eCollection 2023 Feb.

Nakatsukasa K.*, Fujisawa M., Yang X., Kawarasaki T., Okumura F., and Kamura T.*

Triacylglycerol lipase Tgl4 is a stable protein and its dephosphorylation is regulated in a cell cycle-dependent manner in Saccharomyces cerevisiae

Biochemical and Biophysical Research Communications (2022) Aug 11; doi.org/10.1016/j.bbrc.2022.08.022

Obara K., Yoshikawa T., Yamaguchi R., Kuwata K., Nakatsukasa K., Nishimura K., and Kamura T.

Proteolysis of adaptor protein Mmr1 during budding is necessary for mitochondrial homeostasis in Saccharomyces cerevisiae

Nature Communications (2022) Apr 14;13(1):2005. doi: 10.1038/s41467-022-29704-8.

Kusama K., Suzuki Y., Kurita E., Kawarasaki T., Obara K., Okumura F., Kamura T.*, and Nakatsukasa K.*

Dot6/Tod6 degradation fine-tunes the repression of ribosome biogenesis under nutrient-limited conditions

iScience (2022) Feb 26; 25(3):103986. doi: 10.1016/j.isci.2022.103986. eCollection 2022 Mar 18. , Commentary in Japanese

Nakatsukasa K.*, Wigge S., Takano Y., Kawarasaki T., Kamura T., and Brodsky J.L.*

A positive genetic selection for transmembrane domain mutations in HRD1 underscores the importance of Hrd1 complex integrity during ERAD

Current Genetics (2022) Jan 18. doi: 10.1007/s00294-022-01227-1.

Okumura F.*, Oki N., Fujiki Y., Ikuta R., Osaki K., Hamada S., Nakatsukasa K., Hisamoto N., Hara T., Kamura T.*

ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation

Scientific Reports (2021) Oct 22;11(1):20880. doi: 10.1038/s41598-021-00306-6.

Nakatsukasa K.*

Potential Physiological Relevance of ERAD to the Biosynthesis of GPI-Anchored Proteins in Yeast

International Journal of Molecular Sciences (2021) 22(3), 1061; https://doi.org/10.3390/ijms22031061 (registering DOI) 

Okumura F.*, Fujiki Y., Oki N., Osaki K., Nishikimi A., Fukui Y., Nakatsukasa K., and Kamura T.*

Cul5-type Ubiquitin Ligase KLHDC1 Contributes to the Elimination of Truncated SELENOS Produced by Failed UGA/Sec Decoding

iScience (2020) Mar 27;23(3):100970. doi: 10.1016/j.isci.2020.100970. Epub 2020 Mar 7. 

Matsumoto S., Nakatsukasa K., Kakuta C., Tamura Y., Esaki M., and Endo T.*

Msp1 Clears Mistargeted Proteins by Facilitating Their Transfer from Mitochondria to the ER

Molecular Cell (2019) Oct 3;76(1):191-205.e10. doi: 10.1016/j.molcel.2019.07.006. Epub 2019 Aug 21.

Guerriero C.J., Gomez Y.K., Daskivich G.J., Reutter K.R., Augustine A.A., Weiberth K.F., Nakatsukasa K., Grabe M., and Brodsky J.L.*

Harmonizing experimental data with modeling to predict membrane protein insertion in yeast

Biophysical Journal  (2019) Aug 20;117(4):668-678. doi: 10.1016/j.bpj.2019.07.013. Epub 2019 Jul 16. 

Nakatsukasa K.*, Kawarasaki T., and Moriyama A.

Heterologous expression and functional analysis of the F-box protein Ucc1 from other yeast species in Saccharomyces cerevisiae 

Journal of Bioscience and Bioengineering  (2019) Dec;128(6):704-709. doi: 10.1016/j.jbiosc.2019.06.003. Epub 2019 Jun 25. 

Sasako T., Ohsugi M., Kubota N., Itoh S., Okazaki Y, Terai A., Kubota T., Yamashita S., Nakatsukasa K., Kamura T., Iwayama K., Tokuyama K., Kiyonari H., Furuta Y., Shibahara J., Fukayama M., Enooku K., Okushin K., Tsutsumi T., Tateishi R., Tobe K., Asahara H., Koike K., Kadowaki T.* and Ueki K.*

Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism

Nature Communications (2019) volume 10, Article number: 947, 10.1038/s41467-019-08591-6

Nakatsukasa K.*, Sone M., Alemayehu D.H., Okumura F., and Kamura T.*

The HECT-type ubiquitin ligase Tom1 contributes to the turnover of Spo12, a component of the FEAR network, in G2/M phase.

FEBS Letters (2018) May;592(10):1716-1724. doi: 10.1002/1873-3468.13066. Epub 2018 May 7.

Okumura F.*, Joo-Okumura A., Obara K., Petersen A., Nishikimi A., Fukui Y., Nakatsukasa K., and Kamura T.*

Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.

Molecular Biology of the Cell (2017) Nov 15;28(24):3532-3541. doi: 10.1091/mbc.E17-07-0450. Epub 2017 Sep 20. 

Guerriero C.J., Reutter K., Augustine A.A., Preston G.M., Weiberth K.F., Mackie T.D., Cleveland-Rubeor H.C., Bethel N.P., Callenberg K.M., Nakatsukasa K., Grabe M, and Brodsky J.L.*

Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates.

Molecular Biology of the Cell (2017) Jul 15;28(15):2076-2090. doi: 10.1091/mbc.E17-03-0184. Epub 2017 May 24. 

Okumura F.*, Joo-Okumura A., Nakatsukasa K., and Kamura T.*

Hypoxia-inducible factor-2α stabilizes the von Hippel-Lindau (VHL) disease suppressor, Myb-related protein 2.

PLOS ONE (2017) Apr 10;12(4): e0175593. doi: 10.1371/journal.pone.0175593. eCollection. 

<Nagoya University: 2009-2017>

Uematsu K., Okumura F.*, Tonogai S., Joo-Okumura A., Hailu A.D., Nishikimi A., Fukui Y., Nakatsukasa K., and Kamura T.*

ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation.

Journal of Cell Biology (2016) Oct 10;215(1):95-106. 

Okumura, F.*, Joo-Okumura, A., Nakatsukasa, K., and Kamura, T.*

The role of Cullin 5-containing ubiquitin ligases.

Cell division (2016) Mar 9;11:1. doi: 10.1186/s13008-016-0016-3. eCollection. 

Okumura, F.*, Uematsu K., Byrne S.D., Hirano M., Joo-Okumura A., Nishikimi A., Shuin T., Fukui Y., Nakatsukasa K., and Kamura T.*

Parallel regulation of VHL disease by pVHL-mediated degradation of B-Myb and HIF-α.

Molecular and Cellular Biology (2016) May 31;36(12):1803-17. doi: 10.1128/MCB.00067-16.

Nakatsukasa K.*, and Kamura T.*

Subcellular Fractionation Analysis of the Extraction of Ubiquitinated Polytopic Membrane Substrate during ER-Associated Degradation.

PLOS ONE (2016) Feb 5;11(2): e0148327. doi: 10.1371/journal.pone.0148327. eCollection.

Nakatsukasa K.*, Okumura F., Kamura T.*

Proteolytic regulation of metabolic enzymes by E3 ubiquitin ligase complexes: lessons from yeast.

Critical Reviews in Biochemistry and Molecular Biology (2015) Nov-Dec;50(6):489-502. doi: 10.3109/10409238.2015.1081869. Epub 2015 Sep 11. (Invited review)

Nakatsukasa K.*, Nishimura T., Byrne S.D., Okamoto M., Takahashi-Nakaguchi A., Chibana H., Okumura F., and Kamura T.* 

The Ubiquitin Ligase SCFUcc1 Acts as a Metabolic Switch for the Glyoxylate Cycle.

Molecular Cell  (2015) 59, 22-34.

Nakatsukasa K.*, Kamura T., and Brodsky J.L.*

Recent technical developments in the study of ER-associated degradation.

Current Opinion in Cell Biology (2014) 29:82-91. doi: 10.1016/j.ceb.2014.04.008.

Nakatsukasa K.*, Kanada A., Matsuzaki M., Byrne S.D., Okumura F., and Kamura T.* 

The nutrient stress-induced small GTPase Rab5 contributes to the activation of vesicle trafficking and vacuolar activity. 

The Journal of Biological Chemistry (2014) 289, 20970-20978.

Nakatsukasa K.*, Brodsky J.L., and Kamura T.*

A stalled retrotranslocation complex reveals physical linkage between substrate recognition and proteasomal degradation during ER-associated degradation.

Molecular Biology of the Cell (2013) 24, 1765-1775, S1761-1768.

Okumura F.*, Matsuzaki M., Nakatsukasa K., and Kamura T.*

The Role of Elongin BC-Containing Ubiquitin Ligases. 

Frontiers in Oncology (2012) 2, 10. 

Liu Y.†, Nakatsukasa K.†, Kotera M., Kanada A., Nishimura T., Kishi T., Mimura S., and Kamura T.* 

Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function. 

Molecular Biology of the Cell (2011) 22, 1575-1584. 

Nakatsukasa K., and Brodsky J.L.*

In vitro reconstitution of the selection, ubiquitination, and membrane extraction of a polytopic ERAD substrate.

Methods in Molecular Biology (2010) 619, 365-376. 

<University of Pittsburgh: 2004-2009>

Nakatsukasa K., Huyer G., Michaelis S., and Brodsky, J.L.*

Dissecting the ER-associated degradation of a misfolded polytopic membrane protein.

Cell (2008) 132, 101-112.   F1000Prime

Nakatsukasa K., and Brodsky, J.L.*

The recognition and retrotranslocation of misfolded proteins from the endoplasmic reticulum. 

Traffic (2008) 9, 861-870.

Ahner A., Nakatsukasa K., Zhang H., Frizzell R.A., and Brodsky, J.L.*

Small heat-shock proteins select deltaF508-CFTR for endoplasmic reticulum-associated degradation.

Molecular Biology of the Cell (2007) 18, 806-814. 

Nakatsukasa K., and Brodsky J.L.*

The Role of BiP/Kar2p in the translocation of Proteins Across the ER membrane.

The Enzymes (2007) (Academic press, Inc.) edited by Dalbey R.E., Koehler C.M., and Tamanoi F.

Nishikawa S.*, Brodsky J.L., and Nakatsukasa K.

Roles of molecular chaperones in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). 

Journal of Biochemistry (2005) 137, 551-555.

<Nagoya University: 2000-2004>

Nakatsukasa K., Okada S., Umebayashi K., Fukuda R., Nishikawa S., and Endo T.*

Roles of O-mannosylation of aberrant proteins in reduction of the load for endoplasmic reticulum chaperones in yeast.

The Journal of Biological Chemistry (2004) 279, 49762-49772. 

Nakatsukasa K., Nishikawa S., Hosokawa N., Nagata K., and Endo T.*

Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins. 

The Journal of Biological Chemistry (2001) 276, 8635-8638.

<Japanese articles>

Mizushima T.*, and Nakatsukasa K.*

Structural Basis for Metabolic Regulation and Defense Against Ectopic Metabolic Stress by Ubiquitin Ligases

Journal of the Crystallographic Society of Japan (2025) Vol. 67  (2) p. 77-78

Nakatsukasa K.*, and Kamura T.*

ユビキチンリガーゼ複合体SCFUcc1はグリオキシル酸回路の代謝スイッチとして機能する

ライフサイエンス新着レビュー First Authors’ (2015) 

Nakatsukasa K.*, and Kamura T.*

SCFUcc1ユビキチンリガーゼはグリオキシル酸回路の代謝スイッチとして機能する

実験医学 (2015) vol. 33, 2613-2616. 

Okumura F.*, Okumura A., Nakatsukasa K., and Kamura T.*

Elongin BC 型E3ユビキチンリガーゼと細胞機能制御 

生化学 (2013) vol. 85, 76-88. 

Nakatsukasa K.* and Kamura T.

Cullin型E3リガーゼの機能と制御機構

実験医学 (2011) vol. 29, 1933-1937. 

Nakatsukasa K.*

小胞体関連分解(ERAD)における異常タンパク質の認識と逆行輸送

生化学 (2009) vol. 81, 700-703.

Nakatsukasa K.*

タンパク質のOマンノシル化と小胞体品質管理 

実験医学 (2005) vol. 23, 2333-2337.

Nishikawa S., Nakatsukasa K., and Endo T.*

小胞体品質管理におけるシャペロンと糖鎖の役割?何が異常で何を除去すべきか 

蛋白質核酸酵素 (2004) vol. 49, 988-991.