発表論文リスト

Nakano, A., Masuya, T., Akisada, S., Ishikawa-Fukuda, M., Kastuya, M., Mitsuoka, K., Miyoshi, H., Murai, M.*, and Yokoyama, K*. (2026) Structures of respiratory supercomplexes and ATP synthase oligomers in mammalian mitochondrial inner membrane, Nat. Commun. in press.

Ishikawa-Fukuda, M., Seki, T., Kishikawa, J.*, Masuya, T., Kato, T., Okazaki, K., Barquera, B., Miyoshi H., and Murai, M.* (2026) The redox driven Na+-pumping mechanism in Vibrio cholerae NADH-quinone oxidoreductase relies on dynamic conformational changes, Nat. Commun. 17, 1394.（京都大学プレスリリース：電子のやりとりに連動した構造変化が鍵！コレラ菌の生育に必須のナトリウムポンプのはたらく仕組みを解明, EurekAlert!: What drives a mysterious sodium pump? ）

Megaly, AMA., Mokhtar, A., Abdel-Wahab, M., Nakagawa, Y. and Miyashita, M.* (2026) Enhancement of the activity of scorpion short-chain antimicrobial peptides by addition of basic residues at the C-terminus, J. Pestic. Sci. 51, in press.

Nishida, M., Pecorilla, C., Masuya, T.*, Hirano, K., Abe, M, Zdorevskyi, O., Sharma V., Miyoshi, H., and Murai, M.* (2025) Dynamic binding of acetogenin-type inhibitors to mitochondrial complex I revealed by photoaffinity labeling, Biochim. Biophys. Acta (Bioenergetics), 1866, 149566.

Ishikawa-Fukuda, M., Kishikawa, J., Masuya, T., Ito, T., Butler, N. L., McFee, D., Kato, T., Barquera, B., Miyoshi, H., and Murai, M.* (2025) Structural elucidation of the mechanism for inhibitor resistance in the Na+-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae, Biochemistry 64, 1963-1972.

Miyachi, S., Tanaka, H., Ishikawa, M., Mcfee, D., Aoki, W., Murai, M., Barquera, B., Miyoshi, H., and Masuya, T.* (2025) Pinpoint introduction of functional molecular probe into the NqrB subunit of Na+-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae, Biochim. Biophys. Acta (Bioenergetics) 1866, 149551.

Shimase, R., Yoshimoto, Y., Megaly, AMA., Abdel-Wahab, M., Nakagawa, Y. and Miyashita, M.* (2025) Structural determination of Bl-3, an insecticidal peptide from the Buthacus leptochelys scorpion venom, Mass Spectrom. 14, A0180.

Miyashita, M.*, Sakai, S., Okabe, R., Kawai, S., Kishimoto, T., Kitanaka, A., Mitani, N. and Nakagawa, Y. (2025) Identification and synthesis of a long-chain antimicrobial peptide from the venom of the Liocheles australasiae scorpion, J. Pept. Sci. 31, e3661.

Miyashita, M.*, Mitani, N, Iwamoto, F, Hirota M, and Nakagawa, Y. (2025) Discovery of a novel insecticidal peptide with a cystine-stabilized alpha-Helix/alpha-helix motif from the venom of scorpion Liocheles australasiae, Molecules. 30, 32.

Megaly, AMA., Nakamichi, R., Wakayu, M., Nakagawa, Y. Abdel-Wahab, M. and Miyashita, M.* (2025) Identification of a novel insecticidal chlorotoxin-like peptide from the venom of the Compsobuthus egyptiensis scorpion, Toxicon. 267.

Megaly, AMA., Miyashita, M., Alqahtani, AR. and Abdel-Wahab, M.* (2025) Enzymatic characterization and proteomic profiling of venoms from the medically important Androctonus species, J. Genet. Eng. Biotechn. 23, 100566

Kumagai, K., Kishimoto, T., Carleer, K., Butatsu, N., Teramoto, T., Mitani, N., Tytgat, J., Nakagawa, Y. and Miyashita, M.* (2025) LaIT6: A novel insect-selective K+-channel toxin from Liocheles australasiae scorpion venom, Molecules. 30, 3346.

Takeuchi, H., Nomura, M., Horikoshi, R.*, Asano, S., Miyake, T., Kobayashi, T., Murai, M., Chen, Y.-J., Earley, F., Mina, J., and Crossthwaite, A., (2024) Flometoquin, a novel insecticide, acts on mitochondrial complex III as a Qi inhibitor, J. Pestic. Sci. 49, 203-209.

Mizutani, M., Kuroda, S., Oku, M., Aoki, W., Masuya, T., Miyoshi, H., and Murai, M.* (2024) Identification of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe, Biochim. Biophys. Acta (Bioenergetics) 1865, 149147.

Otani, R., Masuya, T., Miyoshi H., and Murai M.* (2024) Mitochondrial respiratory complex I can be inhibited via bypassing the ubiquinone-accessing tunnel, FEBS Lett. 598, 1989-1995.

Sakai, S., Fujita, Y., Juichi, H., Nakagawa, Y. and Miyashita, M.* (2024) Chemical synthesis and functional characterization of LaIT3, an insecticidal two-domain peptide in the Liocheles australasiae venom, Toxicon 238, 107564.

Ikunishi, R., Otani, R., Masuya, T., Shinzawa-Itoh, K., Shiba, T., Murai, M., and Miyoshi, H.* (2023) Respiratory complex I in mitochondrial membrane catalyzes oversized ubiquinones, J. Biol. Chem. 299, 105001. (Editors' Pick)

Tsuji, A., Masuya, T., Arichi N., Inuki, S., Murai, M., Miyoshi, H., and Ohno, H.* (2023) Discovery of bis-sulfonamides as novel inhibitors of mitochondrial NADH-quinone oxidoreductase (complex I), ACS Med. Chem. Lett. 14, 211-216.

宮下正弘 (2023) 「ペプチド農薬の探索研究の動向」化学農薬・生物農薬およびバイオスティミュラントの創製研究動向 (梅津憲治編) pp. 303–311, シーエムシー出版.

Suda, H., Kubo, T., Yoshimoto, Y., Tanaka, K., Tanaka, S., Uchino, A., Azuma, S., Hattori, M., Yamaguchi, T., Miyashita, M., Tominaga,T. and Iwakami, S.* (2023) Transcriptionally linked simultaneous overexpression of P450 genes for broad-spectrum herbicide resistance, Plant Physiology 192, 3017–3029.

Kishikawa, J., Ishikawa, M., Masuya, T., Murai, M., Kitazumi, Y., Butler, N. L., Kato, T., Barquera, B., and Miyoshi, H.* (2022) Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae, Nat. Commun. 13, 4082.（京都大学プレスリリース：コレラ菌の生存に必須である呼吸鎖酵素の構造を解明―病原性細菌に対する新しい抗菌剤の開発研究へ貢献―

Uno, S., Masuya, T., Zdorevskyi, O., Ikunishi, R., Shinzawa-Itoh, K., Lasham, J., Sharma, V., Murai, M., and Miyoshi, M.* (2022) Diverse reaction behaviors of artificial ubiquinones in mitochondrial respiratory complex I, J. Biol. Chem. 298, 102075.

Grba, D. N., Blaza, J. N., Bridges, H. R., Agip, A. A., Yin, Z., Murai, M., Miyoshi, H., and Hirst, J.* (2022) Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I, J Biol Chem. 298, 101602.

Ishikawa, M., Masuya, T., Kuroda, S., Uno, S., Butler, N. L., Foreman, S., Murai, M., Barquera, B., and Miyoshi, M.* (2022) The side chain of ubiquinone plays a critical role in Na+ translocation by NADH-ubiquinone oxidoreductase from Vibrio cholerae, Biochim. Biophys. Acta 1863, 148547.

Unten, Y., Murai, M., Koshitaka, T., Kitao, K., Shirai, O., Masuya, T., and Miyoshi, H.* (2022) Comprehensive understanding of multiple actions of anticancer drug tamoxifen in isolated mitochondria, Biochim. Biophys. Acta 1863, 148520.

Megaly, AMA., Miyashita, M.*, Abdel-Wahab, M., Nakagawa, Y. & Miyagawa, H. (2022) Molecular diversity of linear peptides revealed by transcriptomic analysis of the venom gland of the spider Lycosa poonaensis, Toxins. 14, 854.

Unten, Y., Murai, M.*, Sakai, K., Asami, Y., Yamamoto, T., Masuya, T., and Miyoshi, H. (2021) Natural tetramic acids elicit multiple inhibitory actions against mitochondrial machineries presiding over oxidative phosphorylation, Biosci. Biotechnol. Biochem. 85, 2368-2377.

Sakai, K., Unten, Y., Kimishima, A., Nonaka, K., Chinen, T., Sakai, K., Usui, T., Shiomi, K., Iwatsuki, M., Murai, M., Miyoshi, H., Asami, H.*, and Ōmura, S. (2021) Traminines A and B, produced by Fusarium concentricum, inhibit oxidative phosphorylation in Saccharomyces cerevisiae mitochondria, J. Ind. Microbiol. Biotechnol. 48, kuab051.

Ishikawa, M., Masuya, T., Tanaka, H., Aoki, W., Hantman, N., Butler, N. L., Murai, M., Barquera, B., and Miyoshi, H.* (2021) Specific chemical modification explores dynamic structure of the NqrB subunit in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae, Biochim. Biophys. Acta (Bioenergetics), 1862, 148432

Masuya, T., Uno, S., Murai, M., and Miyoshi, H.* (2021) Pinpoint dual chemical cross-linking explores structural dynamics of the ubiquinone reaction site in mitochondrial complex I, Biochemistry 60, 813-824.

宮下正弘 (2021) 「Venomics手法による生物毒由来の生理活性ペプチドの探索—Omicsを駆使する新たなモノトリ—」日本農薬学会誌. 46, 173–174.

Miyashita, M.*, Mitani, N., Kitanaka, A., Yakio, M., Chen, M., Nishimoto, S., Uchiyama, H., Sue, M., Hotta, H., Nakagawa, Y. and Miyagawa, H. (2021) Identification of an antiviral component from the venom of the scorpion Liocheles australasiae using transcriptomic and mass spectrometric analyses, Toxicon 191, 25–37.

Megaly, AMA., Yoshimoto, Y., Tsunoda, Y., Miyashita, M.*, Abdel-Wahab, M., Nakagawa, Y. and Miyagawa, H. (2021) Characterization of two linear peptides without disulfide bridges from the venom of the spider Lycosa poonaensis (Lycosidae), Biosci. Biotechnol.Biochem. 85, 1348–1356.

Masuya, T., Sano, Y., Tanaka, H., Butler, N. L., Ito, T., Tosaki, T., Morgan, J. E., Murai, M., Barquera, B., and Miyoshi, H.* (2020) Inhibitors of a Na+-pumping NADH-ubiquinone oxidoreductase play multiple roles to block enzyme function, J. Biol. Chem. 295, 12739-12754.

Tsuji, A., Akao, T., Masuya, T., Murai, M., and Miyoshi, H.* (2020) IACS-010759, a potent inhibitor of glycolysis-deficient hypoxic tumor cells, inhibits mitochondrial respiratory complex I through a unique mechanism, J. Biol. Chem. 295, 7481-7491.

Murai, M.* (2020) Exploring the binding pocket of quinone/inhibitors in mitochondrial respiratory complex I by chemical biology approaches, Biosci. Biotechnol. Biochem. 84, 1322-1331. (Review article)

Uno, S., Masuya, T., Shinzawa-Itoh, K., Lasham, J., Haapanen, O., Shiba, T., Inaoka, D. K., Sharma, V., Murai, M., and Miyoshi, H.* (2020) Oversized ubiquinones as molecular probes for structural dynamics of the ubiquinone reaction site in mitochondrial respiratory complex I, J. Biol. Chem. 295, 2449-2463.

Yoshimoto, Y., Tanaka, M., Miyashita, M.*, Abdel-Wahab, M., Megaly, AMA., Nakagawa, Y. and Miyagawa, H. (2020) A fluorescent compound from the exuviae of the scorpion, Liocheles australasiae, J. Nat. Prod. 83, 542–546.

Maruoka, N.*, Watanabe, B., Ando, D., Miyashita, M., Kurihara, T. and Hokazono, H. (2020) Effects of a pyroglutamyl pentapeptide isolated from fermented barley extract on atopic dermatitis-like skin lesions in hairless mouse, Biosci. Biotech. Biochem. 84, 1696–1705.