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SABOURAUD DEXTROSE AGAR

Approximate Formula* Per Liter

Peptic Digest of Animal Tissue........ 5.0 g

Pancreatic Digest of Casein ........... 5.0 g

Dextrose......................................... 40.0 g

Agar................................................ 15.0 g

Eosin Methylene Blue Agar, Modified, Holt-Harris and Teague

Approximate Formula* Per Liter

Pancreatic Digest of Gelatin........... 10.0 g

Lactose............................................. 5.0 g

Sucrose............................................ 5.0 g

Dipotassium Phosphate................... 2.0 g

Eosin Y............................................. 0.4 g

Methylene Blue........................... 65.0 mg

Agar................................................ 13.5 g

Approximate Formula* Per Liter

Pancreatic Digest of Gelatin........... 17.0 g

Peptones (meat and casein)............. 3.0 g

Lactose............................................ 10.0 g

Bile Salts........................................... 1.5 g

Sodium Chloride................................ 5.0 g

Agar................................................. 13.5 g

Neutral Red..................................... 0.03 g

Crystal Violet.................................. 1.0 mg

MANNITOL SALT AGAR

Approximate Formula* Per Liter

Pancreatic Digest of Casein.............. 5.0 g

Peptic Digest of Animal Tissue.......... 5.0 g

Beef Extract....................................... 1.0 g

Sodium Chloride.............................. 75.0 g

D-Mannitol....................................... 10.0 g

Phenol Red.................................. 25.0 mg

Agar................................................. 15.0 g

SALMONELLA SHIGELLA AGAR

Approximate Formula* Per Liter

Beef Extract....................................... 5.0 g

Pancreatic Digest of Casein.............. 2.5 g

Peptic Digest of Animal Tissue.......... 2.5 g

Lactose............................................ 10.0 g

Bile Salts........................................... 8.5 g

Sodium Citrate.................................. 8.5 g

Sodium Thiosulfate........................... 8.5 g

Ferric Citrate..................................... 1.0 g

Agar................................................. 13.5 g

Brilliant Green.............................. 0.33 mg

Neutral Red.................................. 25.0 mg

Tryptic Soy Broth

(Soybean-Casein Digest Medium)

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 17.0 g

Papaic Digest of Soybean................. 3.0 g

Dextrose............................................ 2.5 g

Sodium Chloride................................ 5.0 g

Dipotassium Phosphate.................... 2.5 g

Approximate Formula* Per Liter

Beef Extract....................................... 3.0 g

Peptone............................................. 5.0 g

Lactose.............................................. 5.0 g

Enterococcosel Agar

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 17.0 g

Peptic Digest of Animal Tissue.......... 3.0 g

Yeast Extract..................................... 5.0 g

Oxgall.............................................. 10.0 g

Sodium Chloride................................ 5.0 g

Esculin............................................... 1.0 g

Ferric Ammonium Citrate.................. 0.5 g

Sodium Azide.................................. 0.25 g

Sodium Citrate.................................. 1.0 g

Agar................................................. 13.5 g

Approximate Formula* Per Liter

Peptone............................................. 5.0 g

Yeast Extract..................................... 1.0 g

Ferric Citrate..................................... 0.1 g

Sodium Chloride............................ 19.45 g

Magnesium Chloride......................... 8.8 g

Sodium Sulfate................................ 3.24 g

Calcium Chloride............................... 1.8 g

Potassium Chloride......................... 0.55 g

Sodium Bicarbonate........................ 0.16 g

Potassium Bromide......................... 0.08 g

Strontium Chloride....................... 34.0 mg

Boric Acid..................................... 22.0 mg

Sodium Silicate.............................. 4.0 mg

Sodium Fluoride............................. 2.4 mg

Ammonium Nitrate......................... 1.6 mg

Disodium Phosphate...................... 8.0 mg

Agar................................................. 15.0 g

Approximate Formula* Per Liter

Peptone............................................. 5.0 g

Yeast Extract..................................... 1.0 g

Ferric Citrate..................................... 0.1 g

Sodium Chloride............................ 19.45 g

Magnesium Chloride......................... 5.9 g

Magnesium Sulfate......................... 3.24 g

Calcium Chloride............................... 1.8 g

Potassium Chloride......................... 0.55 g

Sodium Bicarbonate........................ 0.16 g

Potassium Bromide......................... 0.08 g

Strontium Chloride....................... 34.0 mg

Boric Acid..................................... 22.0 mg

Sodium Silicate.............................. 4.0 mg

Sodium Fluoride............................. 2.4 mg

Ammonium Nitrate......................... 1.6 mg

Disodium Phosphate...................... 8.0 mg

Approximate Formula* Per Liter

Beef Extract....................................... 3.0 g

Peptone............................................. 5.0 g

Lactose............................................ 15.0 g

POTATO DEXTROSE AGAR

Approximate Formula* Per Liter

Potato Starch (from infusion)............ 4.0 g

Dextrose.......................................... 20.0 g

Agar................................................. 15.0 g

REINFORCED CLOSTRIDIAL

Approximate Formula* Per Liter

Peptone........................................... 10.0 g

Beef Extract..................................... 10.0 g

Yeast Extract..................................... 3.0 g

Dextrose............................................ 5.0 g

Sodium Chloride................................ 5.0 g

Soluble Starch................................... 1.0 g

Cysteine HCl..................................... 0.5 g

Sodium Acetate................................. 3.0 g

Agar................................................... 0.5 g

BUFFERED PEPTONE WATER

Approximate Formula* Per Liter

Peptone........................................... 10.0 g

Sodium Chloride................................ 5.0 g

Disodium Phosphate......................... 3.5 g

Monopotassium Phosphate.............. 1.5 g

Approximate Formula* Per Liter

Yeast Extract..................................... 0.5 g

Proteose Peptone No. 3.................... 0.5 g

Casamino Acids................................ 0.5 g

Dextrose............................................ 0.5 g

Soluble Starch................................... 0.5 g

Sodium Pyruvate............................... 0.3 g

Dipotassium Phosphate.................... 0.3 g

Magnesium Sulfate......................... 0.05 g

Agar................................................. 15.0 g

Rappaport-Vassiliadis R10 Broth

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 4.54 g

Sodium Chloride................................ 7.2 g

Monopotassium Phosphate............ 1.45 g

Magnesium Chloride (anhydrous)... 13.4 g

Malachite Green Oxalate............. 36.0 mg

LISTERIA ENRICHMENT BROTH

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 17.0 g

Soytone............................................. 3.0 g

Dextrose............................................ 2.5 g

Sodium Chloride................................ 5.0 g

Dipotassium Phosphate.................... 2.5 g

Yeast Extract..................................... 6.0 g

Cycloheximide................................. 0.05 g

Acriflavine HCl.............................. 15.0 mg

Nalidixic Acid................................... 0.04 g

UVM Modified Listeria Enrichment Broth

Approximate Formula* Per Liter

Pancreatic Digest of Casein.............. 5.0 g

Proteose Peptone No. 3.................... 5.0 g

Beef Extract....................................... 5.0 g

Yeast Extract..................................... 5.0 g

Sodium Chloride.............................. 20.0 g

Disodium Phosphate......................... 9.6 g

Monopotassium Phosphate............ 1.35 g

Esculin............................................... 1.0 g

Nalidixic Acid................................... 0.02 g

Acriflavine HCl.............................. 12.0 mg

OXFORD MEDIUM BASE

Approximate Formula* Per Liter

Pancreatic Digest of Casein.............. 8.9 g

Proteose Peptone No. 3.................... 4.4 g

Yeast Extract..................................... 4.4 g

Tryptic Digest of Beef Heart.............. 2.7 g

Starch................................................ 0.9 g

Sodium Chloride................................ 4.4 g

Esculin............................................... 1.0 g

Ferric Ammonium Citrate.................. 0.5 g

Lithium Chloride.............................. 15.0 g

Agar................................................. 15.3 g

Approximate Formula* Per Liter

Proteose Peptone No. 3.................... 1.6 g

Yeast Extract..................................... 3.0 g

L-Lysine............................................. 5.0 g

Xylose............................................. 3.75 g

Lactose.............................................. 7.5 g

Saccharose....................................... 7.5 g

Ferric Ammonium Citrate.................. 0.8 g

Sodium Thiosulfate........................... 6.8 g

Sodium Chloride................................ 5.0 g

Agar................................................. 18.0 g

Phenol Red..................................... 0.08 g

MUELLER HINTON AGAR

Approximate Formula* Per Liter

Beef Extract Powder.......................... 2.0 g

Acid Digest of Casein...................... 17.5 g

Starch................................................ 1.5 g

Agar................................................. 17.0 g

FLUID THIOGLYCOLLATE MEDIUM

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 15.0 g

Yeast Extract..................................... 5.0 g

Dextrose (anhydrous)........................ 5.0 g

Sodium Chloride................................ 2.5 g

L-Cystine........................................... 0.5 g

Sodium Thioglycollate....................... 0.5 g

Agar................................................. 0.75 g

Resazurin....................................... 1.0 mg

TRIPLE SUGAR IRON AGAR

Approximate Formula* Per Liter

Beef Extract....................................... 3.0 g

Yeast Extract..................................... 3.0 g

Pancreatic Digest of Casein............ 15.0 g

Proteose Peptone No. 3.................... 5.0 g

Dextrose............................................ 1.0 g

Lactose............................................ 10.0 g

Sucrose........................................... 10.0 g

Ferrous Sulfate.................................. 0.2 g

Sodium Chloride................................ 5.0 g

Sodium Thiosulfate........................... 0.3 g

Agar................................................. 12.0 g

Phenol Red.................................. 24.0 mg

Desoxycholate Agar

Approximate Formula* Per Liter

Peptone........................................... 10.0 g

Lactose ........................................... 10.0 g

Sodium Desoxycholate..................... 1.0 g

Sodium Chloride................................ 5.0 g

Dipotassium Phosphate.................... 2.0 g

Ferric Ammonium Citrate.................. 1.0 g

Sodium Citrate.................................. 1.0 g

Agar................................................. 15.0 g

Neutral Red..................................... 0.03 g

Approximate Formula* Per Liter

Tryptose.......................................... 20.0 g

Lactose.............................................. 5.0 g

Bile Salts No. 3.................................. 1.5 g

Dipotassium Phosphate.................... 4.0 g

Monopotassium Phosphate.............. 1.5 g

Sodium Chloride................................ 5.0 g

Approximate Formula* Per Liter

Skim Milk Powder.......................... 100.0 g

Approximate Formula* Per Liter

Beef Extract....................................... 3.0 g

Peptone............................................. 5.0 g

TRYPTIC SOY AGAR

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 15.0 g

Papaic Digest of Soybean................. 5.0 g

Sodium Chloride................................ 5.0 g

Agar................................................. 15.0 g

BRAIN HEART INFUSION

Approximate Formula* Per Liter

Calf Brains, Infusion from 200 g........ 7.7 g

Beef Heart, Infusion from 250 g........ 9.8 g

Proteose Peptone........................... 10.0 g

Dextrose............................................ 2.0 g

Sodium Chloride................................ 5.0 g

Disodium Phosphate......................... 2.5 g

Azide Dextrose Broth

Approximate Formula* Per Liter

Beef Extract....................................... 4.5 g

Pancreatic Digest of Casein.............. 7.5 g

Proteose Peptone No. 3.................... 7.5 g

Dextrose............................................ 7.5 g

Sodium Chloride................................ 7.5 g

Sodium Azide.................................... 0.2 g

Brain Heart Infusion Agar

Approximate Formula* Per Liter

Calf Brains, Infusion from 200 g........ 7.7 g

Beef Heart, Infusion from 250 g........ 9.8 g

Proteose Peptone........................... 10.0 g

Dextrose............................................ 2.0 g

Sodium Chloride................................ 5.0 g

Disodium Phosphate......................... 2.5 g

Agar................................................. 15.0 g

Desoxycholate Lactose Agar

Approximate Formula* Per Liter

Proteose Peptone........................... 10.0 g

Lactose ........................................... 10.0 g

Sodium Desoxycholate..................... 0.5 g

Sodium Chloride................................ 5.0 g

Sodium Citrate.................................. 2.0 g

Agar................................................. 15.0 g

Neutral Red..................................... 0.03 g

Approximate Formula* Per Liter

Yeast Extract................................... 10.0 g

Peptone........................................... 20.0 g

Dextrose.......................................... 20.0 g

Agar................................................. 15.0 g

Approximate Formula* Per Liter

Yeast Extract................................... 10.0 g

Peptone........................................... 20.0 g

Dextrose.......................................... 20.0 g

Approximate Formula* Per Liter

Tryptone.......................................... 10.0 g

Yeast Extract..................................... 5.0 g

Sodium Chloride.............................. 10.0 g

Agar................................................. 15.0 g

Approximate Formula* Per Liter

Tryptone.......................................... 10.0 g

Yeast Extract..................................... 5.0 g

Sodium Chloride.............................. 10.0 g

PLATE COUNT AGAR

Approximate Formula* Per Liter

Pancreatic Digest of Casein.............. 5.0 g

Yeast Extract..................................... 2.5 g

Dextrose............................................ 1.0 g

Agar................................................. 15.0 g

POTATO DEXTROSE BROTH

Approximate Formula* Per Liter

Potato Starch (from infusion)............ 4.0 g

Dextrose.......................................... 20.0 g

Agar................................................. 15.0 g

Approximate Formula* Per Liter

Yeast Extract..................................... 5.0 g

Proteose Peptone No. 3.................. 10.0 g

Sodium Citrate................................ 10.0 g

Sodium Thiosulfate......................... 10.0 g

Oxgall................................................ 8.0 g

Saccharose..................................... 20.0 g

Sodium Chloride.............................. 10.0 g

Ferric Ammonium Citrate.................. 1.0 g

Bromthymol Blue............................. 0.04 g

Thymol Blue.................................... 0.04 g

Agar................................................. 15.0 g

Yeast Mold Broth

Approximate Formula* Per Liter

Yeast Extract..................................... 3.0 g

Malt Extract....................................... 3.0 g

Peptone............................................. 5.0 g

Dextrose.......................................... 10.0 g

YEAST MOLD AGAR

Approximate Formula* Per Liter

Yeast Extract..................................... 3.0 g

Malt Extract....................................... 3.0 g

Peptone............................................. 5.0 g

Dextrose.......................................... 10.0 g

Agar................................................. 20.0 g

Approximate Formula* Per Liter

Yeast Extract..................................... 3.0 g

Proteose Peptone No. 3.................. 10.0 g

Lactose............................................ 10.0 g

Saccharose..................................... 10.0 g

Sodium Sulfapyridine........................ 1.0 g

Sodium Chloride................................ 5.0 g

Agar................................................. 20.0 g

Brilliant Green.............................. 12.5 mg

Phenol Red..................................... 0.08 g

Bismuth Sulfite Agar

Approximate Formula* Per Liter

Beef Extract....................................... 5.0 g

Peptone........................................... 10.0 g

Dextrose............................................ 5.0 g

Disodium Phosphate......................... 4.0 g

Ferrous Sulfate.................................. 0.3 g

Bismuth Sulfite Indicator.................... 8.0 g

Agar................................................. 20.0 g

Brilliant Green.............................. 25.0 mg

M Endo Agar LES

Approximate Formula* Per Liter

Yeast Extract..................................... 1.2 g

Casitone............................................ 3.7 g

Thiopeptone...................................... 3.7 g

Tryptose............................................ 7.5 g

Lactose.............................................. 9.4 g

Dipotassium Phosphate.................... 3.3 g

Monopotassium Phosphate.............. 1.0 g

Sodium Chloride................................ 3.7 g

Sodium Desoxycholate..................... 0.1 g

Sodium Lauryl Sulfate..................... 0.05 g

Sodium Sulfite................................... 1.6 g

Basic Fuchsin.................................... 0.8 g

Agar................................................. 15.0 g

BRILLIANT GREEN BILE 2%

Approximate Formula* Per Liter

Peptone........................................... 10.0 g

Oxgall.............................................. 20.0 g

Lactose............................................ 10.0 g

Brilliant Green.............................. 13.3 mg

Approximate Formula* Per Liter

Beef Extract....................................... 5.0 g

Proteose Peptone............................. 5.0 g

Lactose............................................ 10.0 g

Bile Salts No. 3.................................. 8.5 g

Sodium Citrate.................................. 8.5 g

Sodium Thiosulfate........................... 8.5 g

Ferric Citrate..................................... 1.0 g

Agar................................................. 13.5 g

Brilliant Green.............................. 0.33 mg

Neutral Red.................................. 25.0 mg

MUELLER HINTON BROTH

Approximate Formula* Per Liter

Beef Extract Powder.......................... 2.0 g

Acid Digest of Casein...................... 17.5 g

Starch................................................ 1.5 g

BAIRD PARKER AGAR BASE

Approximate Formula* Per Liter

Pancreatic Digest of Casein............ 10.0 g

Beef Extract....................................... 5.0 g

Yeast Extract..................................... 1.0 g

Glycine............................................ 12.0 g

Sodium Pyruvate............................. 10.0 g

Lithium Chloride................................ 5.0 g

Agar................................................. 20.0 g

Approximate Formula* Per Liter

Xylose............................................... 3.5 g

L-Lysine............................................. 5.0 g

Lactose.............................................. 7.5 g

Saccharose....................................... 7.5 g

Sodium Chloride............................... 5.0 g

Yeast Extract..................................... 3.0 g

Phenol Red..................................... 0.08 g

Sodium Desoxycholate..................... 2.5 g

Ferric Ammonium Citrate.................. 0.8 g

Sodium Thiosulfate........................... 6.8 g

Agar................................................. 13.5 g

MacConkey Sorbitol Agar

Approximate Formula* Per Liter

Peptone........................................... 15.5 g

Proteose Peptone............................. 3.0 g

D-Sorbitol........................................ 10.0 g

Bile Salts........................................... 1.5 g

Sodium Chloride................................ 5.0 g

Agar................................................. 15.0 g

Neutral Red..................................... 0.03 g

Crystal Violet.................................. 1.0 mg

Approximate Formula* Per Liter

Beef Extract....................................... 1.0 g

Peptone........................................... 10.0 g

D-Mannitol....................................... 10.0 g

Sodium Chloride.............................. 10.0 g

Phenol Red.................................. 25.0 mg

Agar................................................. 15.0 g

Cetrimide Agar Base

Approximate Formula* Per Liter

Pancreatic Digest of Gelatin........... 20.0 g

Magnesium Chloride......................... 1.4 g

Potassium Sulfate........................... 10.0 g

Cetrimide (Tetradecyltrimethylammonium Bromide)............................................ 0.3 g

Agar................................................. 13.6 g

LACTOBACILLI MRS BROTH

Approximate Formula* Per Liter

Proteose Peptone No. 3.................. 10.0 g

Beef Extract..................................... 10.0 g

Yeast Extract..................................... 5.0 g

Dextrose.......................................... 20.0 g

Polysorbate 80.................................. 1.0 g

Ammonium Citrate............................ 2.0 g

Sodium Acetate................................. 5.0 g

Magnesium Sulfate........................... 0.1 g

Manganese Sulfate......................... 0.05 g

Dipotassium Phosphate.................... 2.0 g

LACTOBACILLI MRS AGAR

Approximate Formula* Per Liter

Proteose Peptone No. 3.................. 10.0 g

Beef Extract..................................... 10.0 g

Yeast Extract..................................... 5.0 g

Dextrose.......................................... 20.0 g

Polysorbate 80.................................. 1.0 g

Ammonium Citrate............................ 2.0 g

Sodium Acetate................................. 5.0 g

Magnesium Sulfate........................... 0.1 g

Manganese Sulfate......................... 0.05 g

Dipotassium Phosphate.................... 2.0 g

Agar................................................. 15.0 g

Yeast Nitrogen Base W/O AA

Approximate Formula* Per Liter

Nitrogen Source

Ammonium Sulfate............................ 5.0 g

Biotin............................................... 2.0 μg

Calcium Pantothenate................. 400.0 μg

Folic Acid......................................... 2.0 μg

Inositol...................................... 2,000.0 μg

Niacin.......................................... 400.0 μg

p-Aminobenzoic Acid.................. 200.0 μg

Pyridoxine Hydrochloride............ 400.0 μg

Riboflavin.................................... 200.0 μg

Thiamine Hydrochloride.............. 400.0 μg

Compounds Supplying Trace Elements

Boric Acid.................................... 500.0 μg

Copper Sulfate.............................. 40.0 μg

Potassium Iodide........................ 100.0 μg

Ferric Chloride............................ 200.0 μg

Manganese Sulfate..................... 400.0 μg

Sodium Molybdate...................... 200.0 μg

Zinc Sulfate................................. 400.0 μg

Monopotassium Phosphate.............. 1.0 g

Magnesium Sulfate........................... 0.5 g

Sodium Chloride................................ 0.1 g

Calcium Chloride............................... 0.1 g

BACTO PEPTONE

Cat No. 211677

TRYPTONE

Cat No. 211705

GRAM STAIN KIT

Cat No. 212539

YEAST EXTRACT

Cat No. 212750

BACTO AGAR 454G

Cat No. 214010

GELATIN

Cat No. 214340

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