Scientific Resources

A.Shrimp Related Platforms

1. Shrimp metabolomics
2. Label-free quantitative proteomics
3. Shrimp cDNA microarray
4. Shrimp 2D protein electrophoresis
5. Shrimp protein identification by LC-nanoESI-MS/MS
6. Shrimp ribosomal protein purification
7. Electrophoresis of shrimp basic proteins
8. Silencing of endogenous target gene in shrimp
9. Primary culture of L. vannamei hemocytes
10. Mitochondrial membrane potential in L. vannamei hemocytes
11. Oxidative stress in L. vannamei hemocytes
12. Immunofluorescent assays of proteins in L. vannamei hemocytes

B.General Platforms

1. Yeast two-hybrid screening
2. Protein interactions using far-Western blotting
3. Co-immunoprecipitation assay (Co-IP)
4. Identification of internal ribosome entry site (IRES) activity
5. A heat-shock expression system in Sf-9 insect cells (Sf-9 cell)
6. Apoptosis analysis in Sf-9 insect cells

C.Shrimp Pathogen Related Platforms

1. WSSV inoculum
2. WSSV virion purification
3. Diagnostic platforms for shrimp white spot disease

D.Extended Information

1. Functional analysis of viral immediate early gene promoters
2. Functional analysis of the regulation mechanism of viral immediate early gene expressions
3. Functional analysis of viral immediate early proteins

E.Future Platform

1. The establishment of shrimp environmental stressors

1. WSSV microarray
2. RNAi library
3. Antibodies against shrimp and WSSV proteins
4. Yeast two-hybrid clones
5. WSSV inoculum