💉 The Order Of Draw
Blood samples must be drawn by phlebotomists in a specific order. This is important to avoid cross-contamination of the sample by additives found in different collection tubes. Cross contamination can occur when blood in an additive tube comes in contact with a needle and when blood is transferred from a syringe into tubes.
Filling tubes in wrong order will cause interference in specimen testing from cross contamination, tissue thromboplastin and microorganisms.
And improperly collected or contaminated specimen can give spurious results (incorrect results). Results may not be reported if it is clear that the result is invalid.Â
This order of draw applies for all specimens collected by syringe, tube holder, or into pre evacuated tubes.
Let the vacuum in the tube fill the blood up to its level.Â
Do not press the syringe plunger as this will lead to hemolysis.
 Gently invert according to the instruction and be sure that the entire inner surface of the blood collection tube is coated with blood while mixing.Â