Micro-Immunoassay

High Sensitivity Micro-Immunoassay Detection: (Quantitative Bioanalysis)

Immunoassays provide a valuable detection and quantification method for biological samples. This work focuses on a novel detection platform utilizing streptavidin-coated silane particles possessing an iron oxide core as a solid capture surface. The introduction of a magnetic field causes supraparticle linear structures to form, and field manipulation produces a periodic change fluorescent signal intensity that can be exploited by signal processing strategies and analyzed independently from unbound analyte.

Publications

Christine F. Woolley and Mark A. Hayes*, Recent Developments in Emerging Microimmunoassays, Bioanalysis, 2013, 5, 245-264.

Prasun Mahanti*, Thomas Taylor*, Mark A. Hayes*, Douglas Cochran, and Matthew M. Petkus. Improved detectability and signal strength for rotating phase fluorescence immunoassays through image processing, Analyst, 2011, 136, 365-373.

Mark A. Hayes*, Matthew M. Petkus, Antonio A. Garcia, Tom Taylor, and Prasun Mahanti. Demonstration of sandwich and competitive modulated supraparticle fluoroimmunoassay applied to cardiac protein biomarker myoglobin, Analyst, 2009, 134, 533-541.

Matthew M. Petkus, Melissa McLauchlin, Anil K. Vuppu, Lynnette Rios, Antonio A. Garcia, and Mark A. Hayes*. Detection of FITC-cortisol via Modulated Supraparticle Lighthouses, Anal. Chem. 2006, 78, 1405-1411.

Future Directions

Incorporate as a detection method for a biomarker panel on a next-generation separations-based microfluidic device.