Electrophoretic Exclusion

Electrophoretic Capture/Separations-Based Arrays: (Bioanalysis, Proteomics, Bioaerosols)

Electrokinetic separations juxtaposed by flow fields represent a valuable tool for separating complex mixtures. A novel separations technique that can dynamically capture specific species in bulk solution from a complex mixture without molecular recognition elements is presented here. The basic premise of the new device is based on electrophoretic principles and exploits differential transport near the capillary entrance by employing a large contraction ratio and setting flow and electric field gradients opposite one another.

Publications

Development of the Resolution Theory for Electrophoretic Exclusion. Stacy M. Kenyon, Michael W. Keebaugh, & Mark A. Hayes Electrophoresis, 2014, 35, 2551-2559 DOI 10.1002/elps.201300572. article

Quantitative assessment of flow and electric fields for electrophoretic focusing at a converging channel entrance with interfacial electrode. Michael Keebaugh, Prasun Mahanti, Mark Hayes, Electrophoresis, 2012, 33(13), 1924-1930. DOI: 10.1002/elps.201200199. article

Using Electrophoretic Exclusion to Manipulate Small Molecules and Particles on a Microdevice. Stacy M. Kenyon, Noah G. Weiss, & Mark A. Hayes Electrophoresis, 2012, 33, 1227-1235, DOI 10.1002/elps.201100622. article

Recent developments in electrophoretic separations on microfluidic devices. Stacy M. Kenyon, Michelle M. Meighan & Mark A. Hayes* Electrophoresis 2011, 32(5), 482-493, DOI: 10.1002/elps.201000469. PMID: 21290388. article

Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins. Michelle M. Meighan, Jared Vasquez, Luke Dziubcynski, Sarah Hews, and Mark A. Hayes* Anal. Chem. 2011, 83, 368-373, DOI: 10.1021/ac1025495. PMID: 21141826. article

Electrophoretic Exclusion for the Selective Transport of Small Molecules. Michelle M. Meighan, Michael W. Keebaugh, Alicia M. Quihuis, Stacy M. Kenyon, and Mark A. Hayes* Electrophoresis 2009, 30, 3786-3792. article

Electrophoretic Focusing Preconcentration Technique in Continuous Buffer Systems Employing Capillary Electrophoresis Separation Systems. N. A. Polson, D. P. Savin, M. A. Hayes* J. Microcol. Separa. 2000, 12, 98-106.

Future Directions

Array-based separations.