We are working on developing a high resolution separations platform capable of distinguishing between subtle variations of proteins. The most significant part of this development includes coupling a mass spectrometer as a detector for capillary isoelectric focusing. We are exploring how this interface works and what are the capabilities of this technique.
Weiss, N.G., Nelson, R.W., and Hayes, M.A. Examining Serum Amyloid P Component Microheterogeneity Using cIEF and MALDI-MS. Proteomics, 2011, 11, 106-113.
Weiss, N.G., Zwick, N., and Hayes, M.A. Capillary Isoelectric Focusing Coupled Offline to MALDI-MS. Journal of Chromatography A, 2010, 1217, 179-182.
Timothy Crowley, M. A. Hayes* Analysis of Human Blood Serum Using the Off-Line Coupling of Capillary Isoelectric Focusing to Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry. Proteomics, 2005, 5(14), 3798-3804.
Unique induced field separations in shaped droplets, direct detection in droplets on hand-held device.