Isoelectric Focusing

Capillary Isoelectric Focussing (serum amyloid P, wt1 protein, Protein Variants)

We are working on developing a high resolution separations platform capable of distinguishing between subtle variations of proteins. The most significant part of this development includes coupling a mass spectrometer as a detector for capillary isoelectric focusing. We are exploring how this interface works and what are the capabilities of this technique.

Publications

Weiss, N.G., Nelson, R.W., and Hayes, M.A. Examining Serum Amyloid P Component Microheterogeneity Using cIEF and MALDI-MS. Proteomics, 2011, 11, 106-113.

Weiss, N.G., Zwick, N., and Hayes, M.A. Capillary Isoelectric Focusing Coupled Offline to MALDI-MS. Journal of Chromatography A, 2010, 1217, 179-182.

Timothy Crowley, M. A. Hayes* Analysis of Human Blood Serum Using the Off-Line Coupling of Capillary Isoelectric Focusing to Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry. Proteomics, 2005, 5(14), 3798-3804.

Future Directions

Unique induced field separations in shaped droplets, direct detection in droplets on hand-held device.