Our research focuses on understanding the mechanisms governing gene regulation in humans in health and disease conditions. In particular, we are interested in unusual nucleic acid structures, called R-loops. R-loops are three-stranded structures formed during transcription, and are composed of an RNA hybridising to a complementary DNA strand, forming an RNA/DNA hybrid, and a displaced single-stranded DNA. R-loops are formed in all living organisms, where they play important biological roles. However, dysregulation of R-loops can have severe cellular consequences, and R-loops are associated with pathology of several human diseases, including cancer and neurodegeneration.