INTRODUCTION:

The iodine test helps to identify the presence of starch in a sample. It's a frequent experiment to be using iodine to verify the presence of starch. The coloration of an iodine as well as potassium iodide liquid solution is bright orange-brown. When it's mixed with a starch-containing sample, like the bread seen above, the color varies to a deep blue or black. (Chemistry Views date unknown). An iodine test can be used for the detection of starch in a given sample. The iodine test can help to distinguish starch. (Biology Online date unknown). A chemical test for starch is to add iodine solution (yellow/brown) and look for a color change. In the presence of starch, iodine turns a blue/black color. It is possible to distinguish starch from glucose (and other carbohydrates) using this iodine solution test. (Bite Size date unknown). The researcher will use 5 different types of starches to see if there is any color reaction when the iodine solution was added to each sample. The objective of the experiment is to identify the presence of starch in the sample provided, using the Iodine test.

STATEMENT OF THE PROBLEM:

• What is the fundamental process of this experimentation?

• What is the justification for the coloration once iodine is added?

• What implication does the starch is present when iodine solution is added in terms of:

- Wheat Granules

- White Rice

- Cube of Potato

- Chopped Onions

- Sponge Cake

MATERIALS AND QUANTITIES:

TABLE 1: Materials and Ingredients that are required to the experiment.

All components used throughout the laboratory activity are readily available in the researcher's home. Because although the researcher possessed the necessary equipment, they were able to save up by not acquiring anything at all from the marketplace. Basically, the resources as well as required equipment are really not complicated to get or use because the ingredients but also materials are commonly accessible, rendering the experimentation practical for anyone eager on attempting it. The researcher also tested the exact measures of the mentioned ingredients to define the accurate portion and to achieve the standards of the experiment's result.

TABLE 1.2: The actual preparation of the materials.

Before commencement, the researcher prepared in an apron as well as gloves to remove impurities and staining from leaving the experimental area. Secondly, the researchers gathered the four miniature containers that would be used as storage for the components used in the test. The four main ingredients, wheat granules, cooked white rice, including chopped onions, were each weighed ½ grams with measuring spoons. Furthermore, to measure the iodine solution, the researcher used a syringe that is perfect for the exact same measurement that the researcher will more likely need for the experimentation of the iodine solution to the starchy foods. The researcher also used the tissues as an optional to wipe all the discarded solution during the experiment. Lastly, the researcher observes the whole experiment and examines the changes on each of the said samples.

PROCEDURES:

FIGURE 1: The Preparation.

FIGURE 1.2: The actual experimentation.

FIGURE 1.3: The actual set-up

RESULTS:

FIGURE 1: Identification of the color and the presence of starch.

FIGURE 1.2: The Before and After Result.

DISCUSSION:

The objective of this experiment should be to ascertain whether or not a substance comprises starches. The starch-iodine test is a modification of this experiment which is used to detect the presence of sugar present in plants throughout the leaves. (Sapkota 2021). Table 1.2 depicts that every one of the substances seems to have the effects of both the absence but also presence of starch, according to the researcher's findings. Starch is a complex carbohydrate that can be found in nature and is widely consumed in the form of wheat, rice, potatoes, and other basic foods grown all over the world. The outcomes from the wheat granules, cooked white rice, potato cube, and sponge cake change color towards blue-black when applying 1cc of iodine solution through using syringe, indicating the presence of starch. Only the chopped onions did not alter color, and the researcher observed that the onions, unlike any other sample, did not have any starch. Because a food with amylase, dextrin, or glycogen indicates the presence of starch, when the researcher adds 1cc of iodine solution to each of the samples, the above mentioned components will turn a dark blue color. The absence of starch in an onion also shows that the onion cell lacks starch storage, preventing it from staining the dark purple. This indicates that the preservation products in potatoes contain starch, whereas the storing compounds in onions do not. (Olabs date unknown).

CONCLUSION:

Afterwards, starch is a carbohydrate that may be discovered as well as are among the most significant pieces of nutritional energy for humans. (Webmd date unknown). Iodine turns a blue/black color when exposed to starch. Using an iodine solution test can tell the difference between starch and glucose and other carbs. (Bite size date unknown). The objective of this experiment was to detect the presence of starch in a food component. After the overall process of experiment, The appearance of a blue-black or purple color represents a positive test which can be seen in the process of experiment that the 4 substances which are the wheat granules, cooked white rice, cube of potato, and sponge cake indicates the presence of starch. If there is no change in color like the chopped onions, the result is negative and indicates the absence of starch.

REFERENCES:

• Biology Online. [Internet]. date unknown. Iodine-test [updated: date unknown, cited: 2021, Nov. 19]. Available from: https://www.biologyonline.com/dictionary/iodine-test

• Bite Size. [Internet]. date unknown. Everyday consumer products. [updated: date unknown, cited: 2021, Nov. 22] Available from: https://www.bbc.co.uk/bitesize/guides/zw37hyc/revision/1#:~:text=A%20chemical%20test%20for%20starch,using%20this%20iodine%20solution%20test.

• Goedecke C. [Internet]. 2016. Why Does Iodine Turn Starch Blue? [updated: 2016, Dec. 6, cited: 2021, Nov. 19]. Available from: https://www.chemistryviews.org/details/education/10128441/Why_Does_Iodine_Turn_Starch_Blue.html#:~:text=Iodine%20Test,changes%20to%20a%20deep%20blue

• Olabs. [Internet]. date unknown. Detection of Starch in Food Samples. [updated: date unknown, cited: 2021, Nov. 22]. Available from: http://amrita.olabs.edu.in/?brch=15&cnt=1&sim=121&sub=79

• Webmd. [Internet]. date unknown. 5 high starch to avoid. [updated: date unknown, cited: 2021, Nov. 22] Available from: https://www.webmd.com/diet/foods-high-in-starch