INTRODUCTION:

Isopropyl alcohol is commonly used as a disinfectant, hand sanitizer, antifreeze, and solvent. People ingest isopropyl alcohol either unintentionally or with the intent to become intoxicated. (Sivilotti 2021). Ethanol is also the intoxicating ingredient of many alcoholic beverages such as beer, wine, and distilled spirits. (Britannica date unknown). When it comes to testing lipids, the ethanol or ethyl alcohol is suggested to determine a lipid in a substance. The emulsion test uses moist compounds to assess the presence of lipids. The process involves suspending the substance in ethanol and permitting any lipids existing to decompose (lipids are soluble in alcohols). After that, the fluid (alcohol with fat dissolved) is decanted into water. (Biology Notes 2014).

STATEMENT OF THE PROBLEM:

• How can isopropyl detect the presence of lipids in a substance?

• What is the main difference between isopropyl and ethyl when it comes to detecting a lipid?

• What is the overall observation for this experimentation?

MATERIALS AND QUANTITIES:

TABLE 1: Materials which are required for the experiment.

Basically, the resources as well as required equipment are really not complicated to get or use because the ingredients but also materials are commonly accessible, rendering the experimentation practical for anyone eager on attempting it.

TABLE 1.2: ACTUAL PREPARATIONS

Before commencement, the researcher prepared in an apron as well as gloves to remove impurities and staining from leaving the experimental area. Secondly, the researchers gathered the five test tube containers that would be used as storage for the canola oil, vinegar, sunflower oil, coconut oil, and corn syrup which is then measured by 1 cc using the syringe. Furthermore, to measure both of the ethyl and isopropyl solution, the researcher used a 3cc syringe that is perfect for the exact same measurement that the researcher will more likely need for the experimentation. After the measurements, the researcher used a stick to stir the samples in different solutions for about 2mins. Lastly, the researcher observes the whole experiment and examines the changes on each of the said samples on different solutions.

PROCEDURES:

FIGURE 1: The Materials/Substances Preparation

FIGURE 1.2: The actual experimentation on isopropyl.

FIGURE 1.3: The actual experimentation on ethyl.

RESULTS:

FIGURE 2: Results for the Isopropyl.

FIGURE 2.1: Overall Results for the Isopropyl Solution.

Canola Oil, Sunflower Oil, Coconut Oil, Vinegar, Corn Syrup

FIGURE 2.2: Actual Results for the Isopropyl Solution.

FIGURE 3: Results for the Ethyl.

FIGURE 3.1: Overall results for the Ethyl Solution.

FIGURE 3.1: Actual results for the Ethyl Solution.

DISCUSSIONS:

The overall discussions for the results of the both solutions (ethyl and isopropyl) were able to detect both absence and the presence of lipids in the 5 substances. Originally, this experiment was to test the presence of lipids in a substance with only the ethyl alcohol solution. But then, accidents happen and the researcher puts the isopropyl solution instead of the ethyl solution in each of the substances. And now, the researcher conducted an experiment which would like to compare the differences between ethyl and isopropyl solutions to test the presence of lipids using two different solutions.

Using the isopropyl solution on the five substances gives the results obtained by detecting the presence of lipids and also the absence of them. The results of this solution acquired that only the sunflower oil has its presence when the isopropyl solution has been added. Also, the vinegar and corn syrup detected its absence on lipids. On the other hand, the remaining two substances which are canola oil and coconut oil have not shown fully detected results for lipids. The result of this experimentation affects its substance with the added solution. This is due to the fact this alcohol also has a portion of its molecule that has no charges, and is nonpolar. When you mix this kind of alcohol with water, the latter's molecules make hydrogen bonds with the water molecules. That is why the results of detecting the substances’ lipids are not that accurate. (Lohner 2017).

Furthermore, using the ethanol solution on the five substances gives the results obtained by detecting the presence of lipids and also the absence of them. The results of this solution acquired that only the vinegar detected its absence on lipids. Vinegar is essentially a dilute solution of acetic (ethanoic) acid in water therefore, the sample vinegar does not contain any lipids. Moreover, the remaining four samples in the experimentation have detected its presence on lipids when the ethanol solution has been added to them. This means that the four substances contain an amount of lipids that is why it is detected as present.

CONCLUSION:

In conclusion, the emulsion test is a chemical precipitation method used to determine the availability of lipids. The process involves suspending the sample in ethanol and allowing any lipids present to disintegrate (lipids are soluble in alcohols). After that, the liquid is decanted into water. (Bite size date unknown). Lipids are hydrocarbon-containing compounds that really are essential to the form and composition of living organisms. (Greenwood 2018). Overall, the Ethanol Emulsion Test is a food test which determines the presence of a broad group of naturally occurring compounds known as lipids. Lipids consist of fats and oils. Other lipid tests include the Grease Spot Test. The Grease spot test is performed on fats lipids which are solid at room temperature.

REFERENCES:

• Up-to-date. [Internet]. date unknown. Isopropyl alcohol poisoning. [updated: date unknown, cited: 2021, Dec. 2]. Available from: https://www.uptodate.com/contents/isopropyl-alcohol-poisoning#:~:text=Isopropyl%20alcohol%20(isopropanol%2C%202%2D,ie%2C%20ethanol%20substitute)%20or%20to

• Britannica. [Internet]. date unknown. Ethanol. [updated: date unknown, cited: 2021, Dec. 2]. Available from: https://www.britannica.com/science/ethanol

• Biology Notes. [Internet]. 2014. Emulsion (ethanol) test for Fats. [updated: 2014, cited: 2021, Dec. 3]. Available from: https://biology-igcse.weebly.com/-food-test-3---emulsion-ethanol-test-for-fats.html

• Lohner S. [Internet]. 2017. Separate Liquids with Salt!. [updated: 2017, Nov. 9 cited: 2021, Dec. 3]. Available from: https://www.scientificamerican.com/article/separate-liquids-with-salt/

• Bite size. [Internet]. date unknown. Enzymes. [updated: date unknown. cited: 2021, Dec. 4]. Available from: https://www.bbc.co.uk/bitesize/guides/z88hcj6/revision/5