INTRODUCTION:

A Biuret test is a chemical test used to determine the presence of a peptide bond in a substance. It is based on the biuret reaction in which a peptide structure containing at least two peptide links produces a violet color when treated with alkaline copper sulfate. (Aryal 2021). Used to implement Biuret's protein testing to analyze food and beverages. A variety of food samples have been manufactured, plus their corresponding solutions have indeed been placed in test tubes. Carry out the experiment with each food sample and keep track of how the color changes over time. Determine the test's positive and negative outcomes. Apply what you've learned to figure out which processed foods proteins. (Labster date unknown).

STATEMENT OF THE PROBLEM:

• Are there any alternate reagents for the Biuret Proteins Assessment?

• What is the procedure for the Biuret Test for protein using sodium hydroxide and copper sulphate solutions?

• What are the expected results in the Biuret Test for proteins?

MATERIALS AND QUANTITIES:

TABLE 1: Materials which are required for the experiment.

Basically, the resources as well as required equipment are really not complicated to get or use because the ingredients but also materials are commonly accessible, rendering the experimentation practical for anyone eager on attempting it. The two solutions which are the Copper Sulfate and Sodium Hydroxide were given by the researchers’ teacher because this type of substance was not easy to find at any store or within the researchers home.

TABLE 1.2: Actual Preparations.

PROCEDURES:

FIGURE 1: The Materials/Substances Preparation.

RESULTS:

FIGURE 2: identifying the presence of proteins.

FIGURE 2.2: Overall results for proteins.

Final results throughout the experimentation.

DISCUSSIONS:

The overall discussion within using the two solutions is that it detected the presence of proteins on the two substances which are the yakult and delight. As the alternative solutions for the biuret test, others can use either Sodium Hydroxide and Copper Sulphate Solutions or the Fehling’s Solutions A and B instead of the Biuret Reagents to get comparable outcomes. (Sapkota 2020). The procedure on identifying the presence of protein in a sample is to first, add 5 ml (or higher) of the liquid food sample to a clean, dry test tube. Next is to apply the measured Sodium Hydroxide to the samples. Whatever is the amount of the sample that you are testing should be the exact measure of the sodium hydroxide solution. Furthermore, apply 10 - 15 drops (or depending on the measurement) of Copper Sulfate to each of the samples. Lastly, stir the solution and observe and also identify the changes of color that are present on the sample. Moreover, Look for color changes in the solution. They range from no color change (blue) to pink to deep violet. Color changes are best visualized against a white background such as a white tile or a sheet of paper. (Biology Notes 2014).

CONCLUSION:

In conclusion, as during examination then when it is imperative to know departure detecting reagents including calibrators owing to also be examined, biuret reagent in the identification of protein applications, impact detection reagents and calibrators will test result. (Bank date unknown). The Biuret test is based on the ability of Cu (II) ions to form a violet-colored chelate complex with peptide bonds (-CONH-groups) in alkaline conditions. Lone electron pairs from 4 nitrogen atoms in the peptide bond coordinate a copper (II) ion to form the chelate complex. The chelate complex absorbs light at 540 nm so appears violet. Hence a color change from blue to violet indicates that proteins are present. The greater the concentration of peptide bonds, the greater the color intensity. If the concentration of peptide bonds is low such as when short-chain peptides are present - the color change is from blue to pink. (ScienceDirect date unknown).

REFERENCES:

• Aryal S. [Internet]. 2021. Biuret test for protein. [Updated: 2021, Nov. 12, cited: 2022 Jan. 25]. Available from: https://microbenotes.com/biuret-test-for-protein

• Labster. [Internet]. Date unknown. Biuret’s Test for Proteins Virtual Lab. [Updated: date unknown, cited: 2022, Jan. 25]. Available from: https://www.labster.com/simulations/biurets-test-for-protein-new/

• Sapkota A. [Internet]. 2020. Fehling’s Test. [Updated: 2020, Nov. 18, cited: 2022, Jan. 25]. Available from: https://microbenotes.com/fehlings-test/

• Biology Notes. [Internet]. 2014. Biuret test for Proteins. [Updated: 2014, cited: 2022, Jan. 25]. Available from: https://biology-igcse.weebly.com/food-test-4---biuret-test-for-proteins.html

• Bank E. [Internet]. Date unknown. What Does a Biuret Test Mean in Biology? [Updated: date unknown, cited: 2022, Jan. 25]. Available from: https://education.seattlepi.com/biuret-test-mean-biology-4659.html

• ScienceDirect. [Internet]. Date unknown. Biuret. [Updated: date unknown, cited: 2022, Jan. 25]. Available from: https://www.sciencedirect.com/topics/chemistry/biuret#:~:text=The%20biuret%20method%20is%20based,the%20amount%20of%20protein%20present.