Biotechnology

Recombinant DNA

Recombinant DNA, often called chimeric DNA, is a hybrid combination of genetic material from different organisms.  The sequence from one organism is cut using restriction enzymes (also known as endonucleases) and spliced using the same enzymes into its host.  The new combination allows for the insertion of a gene to express new proteins and products in the host.  Why perform such a feat of genetic chimerism?  

Well, introducing an icefish gene into tomatoes helps prevent them from freezing.  Alternatively, inserting a gene making plants less tasty to insect parasites saves crops from destruction, helping a greater quantity of food make it to market and assist with efforts to end world hunger.  While genetic engineering might sound blissful, it is not without controversy as studies into its use are ongoing and sometimes concerning.

Watch - Explaining Recombinant DNA Technology

Watch - PCR to Amplify DNA Material

Watch - Genetic Engineering

UberCool! - Editing DNA with CRISPR-Cas9

Watch - Genetically Modified Organisms

Watch - Electrophoresis of DNA with the Amoeba Sisters!

Major Biotech Activity

pGLO - Plasmid Transfer from Jellyfish to Bacteria

Slideshow - here's how it goes down

pGLO Manual - the Lab

When done, and if successful, your bacteria will grow on hostile media and glow under black light.