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2024

• The role of two major nucleoid-associated proteins in Streptomyces, HupA and HupS, in stress survival and gene expression regulation. 

Strzałka A,  Mikołajczyck J., Kowalska K, Skurczyński M, Holmes NA, Jakimowicz D.

doi: 10.1186/s12934-024-02549-0, Microb. Cell Fact., 2024

• The activity of CobB1 protein deacetylase contributes to nucleoid compaction in Streptomyces venezeulae spores by increasing HupS affinity for DNA. 

Duława-Kobeluszczyk J, Strzałka A, Tracz M, Bartyńska M, Pawlikiewicz K, Łebkowski T, Wróbel S, Szymczak J, Zarek A, Jakimowicz D, Szafran MJ.

doi: 10.1093/nar/gkae418,  Nucleic Acids Res., 2024

• MksB is a novel mycobacterial condensin that orchestrates spatiotemporal positioning of replication machinery.

Bułacz H, Hołowka J, Wójcik W, Zakrzewska-Czerwińska J.

doi: 10.1038/s41598-024-70054-w, Sci. Rep., 2024 

2023

• The interplay between the polar growth determinant DivIVA, the segregation protein ParA, and their novel interaction partner PapM controls the Mycobacterium smegmatis cell cycle by modulation of DivIVA subcellular distribution

Matusiak I, Strzałka A, Wadach P, Gongerowska-Jac M, Szwajczak E, Szydłowska-Helbrych A, Kepplinger B, Pióro M, Jakimowicz D

doi: 10.1128/spectrum.01752-23 , Microbiol. Spectr, 2023

• Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach

Noszka M, Strzałka A, Muraszko J, Kolenda R, Meng C, Ludwig C, Stingl K, Zawilak-Pawlik A.

doi: 10.1038/s41467-023-42364-6, Nat Commun, 2023

• Chromosome structure and DNA replication dynamics during the life cycle of the predatory bacterium Bdellovibrio bacteriovorus

Pląskowska K, Zakrzewska-Czerwińska J. 

doi: 10.1093/femsre/fuad057, FEMS Microbiol Rev, 2023

• Binary or Nonbinary Fission? Reproductive Mode of a Predatory Bacterium Depends on Prey Size

Pląskowska K, Makowski Ł, Strzałka A, Zakrzewska-Czerwińska J.

doi: 10.1128/mbio.00772-23, mBio, 2023

• Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin

Hołówka J, Łebkowski T, Feddersen H, Giacomelli G, Drużka K, Makowski Ł, Trojanowski D, Broda N, Bramkamp M, Zakrzewska-Czerwińska J.

doi: 10.3389/fmicb.2023.1146406, Front Microbiol, 2023

• On the mechanisms of lysis triggered by perturbations of bacterial cell wall biosynthesis

Kawai Y, Kawai M, Mackenzie ES, Dashti Y, Kepplinger B, Waldron KJ, Errington J.

doi: 10.1038/s41467-023-39723-8, Nat Commun, 2023

2022

• Enhanced binding of an HU homologue under increased DNA supercoiling preserves chromosome organisation and sustains Streptomyces hyphal growth

Strzałka A, Kois-Ostrowska A, Kędra M, Łebkowski T, Bieniarz G, Szafran MJ, Jakimowicz D.

doi: 10.1093/nar/gkac1093, Nucleic Acids Res, 2022

• Genus-Specific Interactions of Bacterial Chromosome Segregation Machinery Are Critical for Their Function

Pióro M, Matusiak I, Gawek A, Łebkowski T, Jaroszek P, Bergé M, Böhm K, Armitage J, Viollier PH, Bramkamp M, Jakimowicz D.

doi: 10.3389/fmicb.2022.928139, Front Microbiol, 2022

• Mirubactin C rescues the lethal effect of cell wall biosynthesis mutations in Bacillus subtilis

Kepplinger B, Wen X, Tyler AR, Kim BY, Brown J, Banks P, Dashti Y, Mackenzie ES, Wills C, Kawai Y, Waldron KJ, Allenby NEE, Wu LJ, Hall MJ, Errington J.

doi: 10.3389/fmicb.2022.1004737, Front Microbiol, 2022

• Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355

Kepplinger B, Mardiana L, Cowell J, Morton-Laing S, Dashti Y, Wills C, Marrs ECL, Perry JD, Gray J, Goodfellow M, Errington J, Probert MR, Clegg W,  Bogaerts J, Herrebout W, Allenby NEE, Hall MJ.

doi: 10.1038/s41598-022-18726-3, Sci Rep, 2022

• Systematic whole-genome sequencing reveals an unexpected diversity among actinomycetoma pathogens and provides insights into their antibacterial susceptibilities

Watson AK, Kepplinger B, Bakhiet SM, Mhmoud NA, Chapman J, Allenby NE, Mickiewicz K, Goodfellow M, Fahal AH, Errington J.

doi: 10.1371/journal.pntd.0010128, PLoS Negl Trop Dis, 2022

2021

• AdpA Positively Regulates Morphological Differentiation and Chloramphenicol Biosynthesis in Streptomyces venezuelae

Płachetka M, Krawiec M, Zakrzewska-Czerwińska J, Wolański M.

doi: 10.1128/Spectrum.01981-21, Microbiol Spectr, 2021

• Global Chromosome Topology and the Two-Component Systems in Concerted Manner Regulate Transcription in Streptomyces

Gongerowska-Jac M, Szafran MJ, Mikołajczyk J, Szymczak J, Bartyńska M, Gierlikowska A, Biały S, Elliot MA, Jakimowicz D.

doi: 10.1128/mSystems.01142-21, mSystems, 2021

• Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development

Szafran MJ, Małecki T, Strzałka A, Pawlikiewicz K, Duława J, Zarek A, Kois-Ostrowska A, Findlay KC, Le TBK, Jakimowicz D

doi: 10.1038/s41467-021-25461-2, Nat Commun, 2021

• Cholesterol-dependent transcriptome remodeling reveals new insight into the contribution of cholesterol to Mycobacterium tuberculosis pathogenesis

Pawełczyk J, Brzostek A, Minias A, Płociński P, Rumijowska-Galewicz A, Strapagiel D, Zakrzewska-Czerwińska J, Dziadek J.

doi: 10.1038/s41598-021-91812-0, Sci Rep, 2021

• Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces

Gongerowska-Jac M, Szafran MJ, Jakimowicz D.

doi: 10.1186/s12934-021-01590-7, Microb Cell Fact, 2021

• Lsr2 and Its Novel Paralogue Mediate the Adjustment of Mycobacterium smegmatis to Unfavorable Environmental Conditions

Kołodziej M, Łebkowski T, Płociński P, Hołówka J, Paściak M, Wojtaś B, Bury K, Konieczny I, Dziadek J, Zakrzewska-Czerwińska J.

doi: 10.1128/mSphere.00290-21, mSphere, 2021

• Thiosemicarbazide Derivatives Decrease the ATPase Activity of Staphylococcus aureus Topoisomerase IV, Inhibit Mycobacterial Growth, and Affect Replication in Mycobacterium smegmatis

Kowalczyk A, Paneth A, Trojanowski D, Paneth P, Zakrzewska-Czerwińska J, Stączek P.

doi: 10.3390/ijms22083881, Int J Mol Sci, 2021

• Genetic Engineering in Combination with Semi-Synthesis Leads to a New Route for Gram-Scale Production of the Immunosuppressive Natural Product Brasilicardin A

Botas A, Eitel M, Schwarz PN, Buchmann A, Costales P, Núñez LE, Cortés J, Morís F, Krawiec M, Wolański M, Gust B, Rodriguez M, Fischer WN, Jandeleit B, Zakrzewska-  Czerwińska J, Wohlleben W, Stegmann E, Koch P, Méndez C, Gross H.

doi: 10.1002/anie.202015852, Angew Chem Int Ed Engl, 2021

• Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle

Kołodziej M, Trojanowski D, Bury K, Hołówka J, Matysik W, Kąkolewska H, Feddersen H, Giacomelli G, Konieczny I, Bramkamp M, Zakrzewska-Czerwińska J.

doi: 10.1038/s41598-021-82295-0, Sci Rep, 2021

2020

• A novel LysR-type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin

Wolański M, Krawiec M, Schwarz PN, Stegmann E, Wohlleben W, Buchmann A, Gross H, Eitel M, Koch P, Botas A, Méndez C, Núñez LE, Morís F, Cortés J, Zakrzewska- Czerwińska J.

doi: 10.1002/elsc.202000038, Eng Life Sci, 2020

• Functional Disassociation Between the Protein Domains of MSMEG_4305 of Mycolicibacterium smegmatis (Mycobacterium smegmatis) in vivo

Czubat B, Minias A, Brzostek A, Żaczek A, Struś K, Zakrzewska-Czerwińska J, Dziadek J.

doi: 10.3389/fmicb.2020.0200, Front Microbiol, 2020

• Compaction and control-the role of chromosome-organizing proteins in Streptomyces

Szafran MJ, Jakimowicz D, Elliot MA.

doi: 10.1093/femsre/fuaa028, FEMS Microbiol Rev, 2020

• Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Makowski Ł, Trojanowski D, Zakrzewska-Czerwińska J.

doi: 10.3791/61105, J Vis Exp, 2020

• Nucleoid Associated Proteins: The Small Organizers That Help to Cope With Stress

Hołówka J, Zakrzewska-Czerwińska J.

doi: 10.3389/fmicb.2020.00590, Front Microbiol, 2020

• Chromosome Segregation Proteins as Coordinators of Cell Cycle in Response to Environmental Conditions

Pióro M, Jakimowicz D.

doi: 10.3389/fmicb.2020.00588,  Front Microbiol, 2020

• AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor

Łebkowski T, Wolański M, Ołdziej S, Flärdh K, Zakrzewska-Czerwińska J.

doi: 10.1128/JB.00597-19, J Bacteriol, 2020

2019

• Molekularne podstawy organizacji chromatyny bakteryjnej [Molecular mechanisms involved in bacterial chromatin organization].

Kołodziej M, Zakrzewska-Czerwińska J, Hołówka J.

doi: 10.18388/pb.2019_270, Postepy Biochem, 2019

• Streptomycete origin of chromosomal replication with two putative unwinding elements

Płachetka M, Żyła-Uklejewicz D, Weigel C, Donczew R, Donczew M, Jakimowicz D, Zawilak-Pawlik A, Zakrzewska-Czerwinska J.

doi: 10.1099/mic.0.000859, Microbiology (Reading), 2019

• A highly processive actinobacterial topoisomerase I - thoughts on Streptomyces' demand for an enzyme with a unique C-terminal domain

Szafran MJ, Strzałka A, Jakimowicz D.

doi: 10.1099/mic.0.000841, Microbiology (Reading), 2020

• Dynamics of Chromosome Replication and Its Relationship to Predatory Attack Lifestyles in Bdellovibrio bacteriovorus

Makowski Ł, Trojanowski D, Till R, Lambert C, Lowry R, Sockett RE, Zakrzewska-Czerwińska J.

doi: 10.1128/AEM.00730-19, Appl Environ Microbiol, 2019

• Transcriptional Response of Streptomyces coelicolor to Rapid Chromosome Relaxation or Long-Term Supercoiling Imbalance

Szafran MJ, Gongerowska M, Małecki T, Elliot M, Jakimowicz D.

doi: 10.3389/fmicb.2019.01605, Front Microbiol, 2019

• Watching DNA Replication Inhibitors in Action: Exploiting Time-Lapse Microfluidic Microscopy as a Tool for Target-Drug Interaction Studies in Mycobacterium

Trojanowski D, Kołodziej M, Hołówka J, Müller R, Zakrzewska-Czerwińska J.

doi: 10.1128/AAC.00739-19, Antimicrob Agents Chemother, 2019

2018

•  Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective
  Trojanowski D, Hołówka J, Zakrzewska-Czerwińska J.
  doi: 10.3389/fmicb.2018.02819,  Front Microbiol, 2018

• Competition between DivIVA and the nucleoid for ParA binding promotes segrosome separation and modulates mycobacterial cell elongation
  Pióro M, Małecki T, Portas M, Magierowska I, Trojanowski D, Sherratt D, Zakrzewska-Czerwińska J, Ginda K, Jakimowicz D.
  doi: 10.1111/mmi.14149, Mol Microbiol, 2018

• Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), Impair M. smegmatis Growth and Disturb Chromosome Replication
  Szafran MJ, Kołodziej M, Skut P, Medapi B, Domagała A, Trojanowski D, Zakrzewska-Czerwińska J, Sriram D, Jakimowicz D.
  doi: 10.3389/fmicb.2018.01592, Front Microbiol, 2018

2017

• Hołówka J, Trojanowski D, Janczak M, Jakimowicz D, Zakrzewska-Czerwińska J The Origin of Chromosomal Replication Is Asymmetrically Positioned on the Mycobacterial Nucleoid, and the Timing of Its Firing Depends on HupB.J Bacteriol. 2018 Apr 24;200(10). pii: e00044-18. doi: 10.1128/JB.00044-18. 

• Hołówka J, Trojanowski D, Ginda K, Wojtaś B, Gielniewski B, Jakimowicz D, Zakrzewska-Czerwińska J HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like TailMBio. 2017 Nov 7;8(6). pii: e01272-17. doi: 10.1128/mBio.01272-17.

• Strzalka A, Szafran MJ, Strick T, Jakimowicz D C-terminal lysine repeats in Streptomyces topoisomerase I stabilize the enzyme-DNA complex and confer high enzyme processivity. Nucleic Acids Res. 2017 Nov 16;45(20):11908-11924.

• Ginda K, Santi I, Bousbaine D, Zakrzewska-Czerwińska J, Jakimowicz D, McKinney J. The studies of ParA and ParB dynamics reveal asymmetry of chromosome segregation in Mycobacteria. Mol Microbiol. 2017 May 18. doi: 10.1111/mmi.13712. 

• Zawilak-Pawlik A, Nowaczyk M, Zakrzewska-Czerwińska J. The Role of the N-Terminal Domains of Bacterial Initiator DnaA in the Assembly and Regulation of the Bacterial Replication Initiation Complex. Genes (Basel). 2017 May 10;8(5). pii: E136. doi: 10.3390/genes8050136. Review. PubMed PMID: 28489024; PubMed Central PMCID: PMC5448010.

• Trojanowski D, Hołówka J, Ginda K, Jakimowicz D, Zakrzewska-Czerwińska J. Multifork chromosome replication in slow-growing bacteria. Scientific Reports. 2017 Mar 6. doi:10.1038/srep43836

• Zawilak-Pawlik A, Zakrzewska-Czerwińska J. Recent Advances in Helicobacter pylori Replication: Possible Implications in Adaptation to a Pathogenic Lifestyle and Perspectives for Drug Design. Curr Top Microbiol Immunol. 2017;400:73-103. doi: 10.1007/978-3-319-50520-6_4. PMID: 28124150

2016 and before

• Buchmann A, Eitel M, Koch P, Schwarz PN, Stegmann E, Wohlleben W, Wolański M, Krawiec M, Zakrzewska-Czerwińska J, Méndez C, Botas A, Núñez LE, Morís F, Cortés J, Gross H. High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies. Genome Announc. 2016 Dec 15;4(6). pii: e01391-16. doi: 10.1128/genomeA.01391-16. PMID: 27979943

• Kois-Ostrowska A, Strzałka A, Lipietta N, Tilley E, Zakrzewska-Czerwińska J, Herron P, Jakimowicz D. Unique Function of the Bacterial Chromosome Segregation Machinery in Apically Growing Streptomyces - Targeting the Chromosome to New Hyphal Tubes and its Anchorage at the Tips. PLoS Genet. 2016 Dec 15;12(12):e1006488. doi: 10.1371/journal.pgen.1006488

• Makowski Ł, Donczew R, Weigel C, Zawilak-Pawlik A, Zakrzewska-Czerwińska J. Initiation of Chromosomal Replication in Predatory Bacterium Bdellovibrio bacteriovorus. Front Microbiol. 2016 Nov 28;7:1898. eCollection 2016. PMID: 27965633

• Szafran MJ, Gongerowska M, Gutkowski P, Zakrzewska-Czerwińska J, Jakimowicz D. The coordinated positive regulation of topoisomerase genes maintains topological homeostasis in Streptomyces coelicolor. J Bacteriol.2016 Aug 22. pii: JB.00530-16.

• Donczew M, Mackiewicz P, Wróbel A, Flärdh K, Zakrzewska-Czerwińska J, Jakimowicz D. ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation. Open Biol.2016 Apr;6(4):150263. doi: 10.1098/rsob.150263. Epub 2016 Apr 27.

• Wolański M, Łebkowski T, Kois-Ostrowska A, Zettler J, Apel AK, Jakimowicz D, Zakrzewska-Czerwińska J. Two transcription factors, CabA and CabR, are independently involved in multilevel regulation of the biosynthetic gene cluster encoding the novel aminocoumarin, cacibiocin. Appl Microbiol Biotechnol. 2016 Apr;100(7):3147-64. doi: 10.1007/s00253-015-7196-7. Epub 2015 Dec 5.

• Baronian G, Ginda K, Berry L, Cohen-Gonsaud M, Zakrzewska-Czerwińska J, Jakimowicz D, Molle V. Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.PLoS One. 2015 Mar 25;10(3):e0119907. doi: 10.1371/journal.pone.0119907. eCollection 2015.

• Trojanowski D, Ginda K, Pióro M, Hołówka J, Skut P, Jakimowicz D, Zakrzewska-Czerwińska J. Choreography of the Mycobacterium replication machinery during the cell cycle.MBio. 2015 Feb 17;6(1):e02125-14. doi: 10.1128/mBio.02125-14. 

• Wolański M, Donczew R, Zawilak-Pawlik A, Zakrzewska-Czerwińska J. oriC-encoded instructions for the initiation of bacterial chromosome replication.Front Microbiol. 2015 Jan 6;5:735. doi: 10.3389/fmicb.2014.00735. eCollection 2014.

• Donczew R, Makowski Ł, Jaworski P, Bezulska M, Nowaczyk M, Zakrzewska-Czerwińska J, Zawilak-Pawlik A. The atypical response regulator HP1021 controls formation of the Helicobacter pylori replication initiation complex.Mol Microbiol. 2015 Jan;95(2):297-312. doi: 10.1111/mmi.12866. Epub 2014 Dec 19. 

• Wolański M, Jakimowicz D, Zakrzewska-Czerwińska J Fifty years after the replicon hypothesis: cell-specific master regulators as new players in chromosome replication control.J Bacteriol. 2014 Aug 15;196(16):2901-11. doi: 10.1128/JB.01706-14. Epub 2014 Jun 9. Review. PMID: 24914187

• Szafran MJ, Strick T, Strzałka A, Zakrzewska-Czerwińska J, Jakimowicz D. A highly processive topoisomerase I: studies at the single-molecule level.Nucleic Acids Res. 2014 Jul;42(12):7935-46. doi: 10.1093/nar/gku494. Epub 2014 May 31.

• Donczew R, Mielke T, Jaworski P, Zakrzewska-Czerwińska J, Zawilak-Pawlik A. Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions.J Mol Biol. 2014 Jul 29;426(15):2769-82. doi: 10.1016/j.jmb.2014.05.018. Epub 2014 May 24. PMID: 24862285

• Rowinska-Zyrek M, Zakrzewska-Czerwinska J, Zawilak-Pawlik A, Kozlowski H. Ni²⁺ chemistry in pathogens--a possible target for eradication.Dalton Trans. 2014 Jun 28;43(24):8976-89. doi: 10.1039/c4dt00421c. Erratum in: Dalton Trans. 2014 Nov 28;43(44):16943.PMID: 24781528

• Donczew R, Zakrzewska-Czerwińska J, Zawilak-Pawlik A. Beyond DnaA: the role of DNA topology and DNA methylation in bacterial replication initiation.J Mol Biol. 2014 Jun 12;426(12):2269-82. doi: 10.1016/j.jmb.2014.04.009. Epub 2014 Apr 18. Review. PMID: 24747048

• Masiewicz P, Wolański M, Brzostek A, Dziadek J, Zakrzewska-Czerwińska J. Propionate represses the dnaA gene via the methylcitrate pathway-regulating transcription factor, PrpR, in Mycobacterium tuberculosis.Antonie Van Leeuwenhoek. 2014 May;105(5):951-9. doi: 10.1007/s10482-014-0153-0. Epub 2014 Apr 5.

• Szafran M, Skut P, Ditkowski B, Ginda K, Chandra G, Zakrzewska-Czerwińska J, Jakimowicz D. Topoisomerase I (TopA) is recruited to ParB complexes and is required for proper chromosome organization during Streptomyces coelicolor sporulation.J Bacteriol. 2013 Oct;195(19):4445-55. doi: 10.1128/JB.00798-13. Epub 2013 Aug 2.