Who should attend?

M.B.B.S./M.Sc/M.Tech/M.D./Ph.D. students, faculty members, biomedical scientists, agricultural, veterinary scientists, clinicians from any branch of life sciences or a relevant field. Maximum no. of participants for the workshop will be restricted to 20. Candidate selection will be based on first come first serve basis.

Registration Procedure

Rs: 4500 (Four thousand five hundred only)

pay by cash/ direct deposit to our account

Ac.No: 104001011005868

Name of Ac Holder: Prashantha C N

Bank Name: Vijaya Bank

IFSC Code: VIJB0001040

Branch: Byatarayanapura, Bangalore-560092

Course Objectives

After this workshop participants should:

• More familiar with the tools and means to construct workflows/pipelines for WES data analysis
• To understand the file formats produced at each stage of the bioinformatic pipeline (BAM, fastq,vcf).
• To gain practical skills in applying standard bioinformatic tools, such as alignment to a reference sequence, variant calling and annotation.
• To identify genes associated with a phenotype todesigna gene panel which can be applied to whole exome data.
• Gain experience in analysis and interpretation of NGS variants
• Understand the quality control of sequencing data.

Course Syllabus

- Introduction to NGS analysis methods

- Introduction to R and Unix

- Raw Data Selection (NGS Data selection, principles, type of data, file formates)

- Quality analysis of raw data

- Pre-processing of raw data (Trim adaptors & Contaminants, Filter by quality scores, filter duplicate reads)

- Mapping reads onto a reference genome

- Targeted sequencing quality control

- Quality control of mapped reads

- Post alignment processing

- Variant calling

- Effect annotation of the found variants

- Variant prioritisation in Variant Explorer