SERVICE WORKFLOW FAQS

1). Where is your company located?

From our headquarters is located in Bangalore and the Incubation center is located in Institute of Agri-Biotechnology (IABT). University of Agricultural Sciences, Dharwad, Karnataka, India. Clovergen is committed to quality service and scientific excellence.

We have state-of-the-art sequencing centers in Institute of Agri-Biotechnology (IABT). University of Agricultural Sciences, Dharwad, Karnataka, India.

2). Do you have a location where samples can be shipped?

Yes. You can ship samples to our Sequencing Center in Clovergen office, Dharwad. The Address is as follows:

Clovergen Life Sciences Pvt Ltd

Dr.C.N.Prashanha

Lab No: 3, KBITS Incubation center

Institute of Agri-Biotechnology (IABT)

University of Agricultural Sciences,

Dharwad-580005, Karnataka, India.

Tel.No: +91-836-2449444

Mobile: +91-9844158444

Email: clovergenls@gmail.com

3). Where do you conduct the sample QC?

Currently, we perform samples QC tests and bioinformatics analysis is conducted both in Bangalore and Dharwad Address. Depending on which location your samples will be sequenced. We recommend all customers to ship samples to our Dharwad Genomics lab if needed.

4). How do I ship my samples to Dharwad?

Clovergen has abundant experience in the delivery of samples to Dharwad from worldwide locations, and we have a straightforward delivery process. When you prepare to send samples, please fill out the Sample Information Form carefully so that we receive complete and accurate information on your samples. For more detailed information, please see our Sample Pre-QC and Shipping Instructions.

5). What is your service workflow?

Once your samples arrive at our laboratory, sample quality control testing will be started immediately. However, library construction and sequencing will begin only after we receive your Purchase Order (PO). After your project is completed, an invoice will be sent to you via email. Please follow the instructions on the invoice for payments.

6). How do I pay for your sequencing services?

Clovergen accepts three payment methods, as follows:

1. Via electronic transfer. Bank account information will be shown on the invoice.
2. By credit card. Please note that there is a credit card processing fee.
3. By check. Mail your check directly to our Bangalore office at the address below:

Clovergen Life Sciences Pvt Ltd

#165, 4^th Main, 2^nd B Cross, Talakavery Layout, Amruthahalli, Bangalore-560092

Tel.No: +91-80-41550847

Mobile: +91-9844158444

Email: clovergenls@gmail.com

7). Will Clovergen maintain the data obtained from my samples confidentially and securely?

At Clovergen, we place the highest priority on the confidentiality and security of your data and intellectual property. As an established private company, maintaining the integrity of our customers’ data is critical to our business. Per our policies, your data will not be disclosed to any other parties or organizations without your authorization and will be deleted from our databases within a specified period following delivery to you. Upon request, we will provide you with a written agreement that has clearly defined terms of confidentiality.

8). What methods can be used to send DNA and RNA samples?

You have the following options for shipping DNA samples:

• The DNA can be lyophilized for shipping at room temperature.
• Samples can be packed with ice packs/blue ice.
• Best method: pack samples in dry ice, using sufficient dry ice to allow for the consumption (sublimation) of 5 kg per day, through Blue Dart Courier services or other carrier of choice.

You have the following options for shipping RNA samples:

1. Lyophilize the RNA before shipping at 2-8 °C or at room temperature.
2. Pack samples in dry ice, using sufficient dry ice to allow for the consumption (sublimation) of 5 kg per day, through BlueDart or other carrier of choice.
3. Stabilize RNA using RNAlater® (for tissue samples) or RNAstable® (for extracted RNA samples) and ship at room temperature.

9). What other types of samples do you accept? Can I send tissues or body fluids for you to perform the DNA/RNA extractions?

We provide DNA/RNA extraction services for tissues or body fluids, plant samples or microbial samples. However, we encourage you to send us purified DNA or RNA samples. If you have a need for DNA/RNA extractions, please contact your Clovergen representative to discuss your specific needs.

10). What kits do you use for DNA/RNA library preparation?

1. DNA library prep kit: We have customized DNA Library Prep Kit
2. RNA library prep kit: We have customized RNA Library Prep Kit
3. PCR-free DNA library prep kit: TruSeq DNA PCR-Free Library Preparation Kit

11). What are your platforms for sequencing?

The sequencing platforms used at Clovergen include Ion-Torrent, Genetic analyzer. We employ a collaborative model where we partner with the Certified Genomics Service provider both in India and Abroad to generate best quality data for your project. We have access to to all the available platform which includes Illumina Hiseq 2000, Illumina Miseq, Ion Proton, Ion Torrent, Roche FLX, Roche FLX plus and PacBio, Illumina iScan and Agilent Microarray Scanner This means that we do not have to be biased about a particular platform and based on your study we will provide you the option of the most suited platform for your typical experiment.

We have 4 companies collaborations to provide sequencing services having different platforms include HiSeq X Ten, HiSeq 4000, HiSeq2500/HiSeq2000, MiSeq, and NextSeq500. These platforms can produce high quality data using different sequencing strategies, and are tailored to meet the diverse needs (library types, read length, throughput, turnaround time and cost) of scientific research and applications.

We have one of the largest sequencing capacities in the world, including 20 HiSeq X instruments at our Dharwad lab. The sequencing platforms used at our Clovergen sequencing center include Ion Torrent and Genetic Analyzer.

12). How do I obtain the data after the project is completed?

We offer three options for data delivery:

• Hard drive/U-disk sent via BlueDart / Professional courier services
• Data download through our secure FTP server
• Google cloud data download

13). When the sequencing is completed, can you return the remainder of the samples to me?

Yes, if you pay the transport charge, we can return the remainder of the samples to you.

14). When the sequencing is completed, can you return the remainder of the libraries to me?

If the libraries have been constructed by Clovergen, we do not return the remainder of the libraries.