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Vitrification and cryopreservation methods to preserve organs, tissues, and cells for long-term use.
Vitrification and cryopreservation methods to preserve organs, tissues, and cells for long-term use.
Our Mission
Our Mission
Our lab is pioneering next-generation cryopreservation techniques to overcome the challenges of organ and tissue storage for transplantation, regenerative medicine, and biobanking. By developing advanced vitrification and rewarming technologies, we aim to enable long-term preservation without ice damage, ensuring the viability and function of biological systems after thawing.
Our Impact
Our Impact
Our work has profound implications for organ transplantation, regenerative medicine, and species conservation. By refining these techniques, we aim to develop scalable, clinically viable solutions for long-term organ storage, reducing transplant wait times and expanding donor organ availability.
Research Areas
Research Areas
Cryomesh Technology for Vitrification
We are developing conduction-dominated cryomesh structures that facilitate rapid and uniform cooling, enabling successful vitrification of tissues and small organisms. This breakthrough method minimizes ice formation, making it a promising solution for organ banking and biomedical storage.
Rapid Joule Heating for Safe Rewarming
One of the biggest barriers to effective cryopreservation is the risk of damage during rewarming. Our research on Joule heating demonstrates how rapid, controlled rewarming can prevent ice recrystallization and thermal stress, significantly improving the survival of vitrified tissues.
Large-Scale Vitrification & Nanowarming
Scaling up vitrification for whole organs requires innovative approaches to both freezing and rewarming. Our lab has successfully demonstrated liter-scale vitrification using advanced cryoprotectant perfusion systems, alongside nanoparticle-based warming driven by high-power radiofrequency (RF) fields. These advances bring us closer to preserving large biological systems for transplantation.
Magnetic Nanoparticles for Organ Cryopreservation
We are leveraging magnetic nanoparticles for precise, uniform rewarming of vitrified organs, including rat hearts, with the goal of achieving whole-organ cryopreservation. This technique ensures even heat distribution, reducing the risk of thermal stress and tissue damage, a critical step toward clinical translation.
Conduction-Dominated Cryomesh for Organism Vitrification. Advanced Science. 11 (3), [2303317]. Guo, Z., Zuchowicz, N., Bouwmeester, J., Joshi, A.S., Neisch, A.L., Smith, K., Daly, J., Etheridge, M.L., Finger, E.B., Kodandaramaiah, S.B., Hays, T.S., Hagedorn, M., Bischof, J.C. (2024).
Rapid Joule Heating Improves Vitrification Based Cryopreservation. Zhan, L., Han, Z., Shao, Q., Etheridge, M., Hays, T., Bischof, J. C. Nature Communications, 2022.
Physical Demonstration of Vitrification of Liter Scale CPA Systems and Characterization of 120KW RF Coil for Nanowarming of Liter Scale Systems. Gangwar, L., Han, Z., Hintz, M., Pasek-Allen, J., Etheridge, M., Goldstein, R., Bischof, J. Cryobiology, 2022.
Vitrification and Rewarming of Magnetic Nanoparticle-Loaded Rat Hearts. Namsrai, B., Gao, Z., Han, Z., Joshi, P., Rao, J., Ravikumar, V., Sharma, A., Ring, H., Idiyatullin, D., Magnuson, E., Iaizzo, P. A., Tolkacheva, E.G., Garwood, M., Rabin, Y., Etheridge, M., Finger, E., Bischof, J.C. Cyrobiology, 2022.
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