Corneal Wound Healing

Our principal aim is to elucidate the effects of various ophthalmic solutions to test in vitro cellular proliferation, protein expression, and migration ability within a wound-healing model. This aim focuses on examining the effects different ophthalmic solutions have on epithelial cell lines. Epithelial cell lines such as A549s, ocular cell lines like human corneal epithelial  cells (HCEC), or fibroblast-like cells will be used in this study. Our goal is to measure and determine the greatest growth rate and migratory ability by developing growth curves before and after treatments with various ophthalmic solutions. We will observe protein expression by Western blotting as a form of biochemical analysis. Moreover, to test cell migration ability, traditional cell scraping protocols will be followed. These tests will enable us to determine which ophthalmic solution provides the best wound-healing results.