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1. Metabolically engineered E. coli, C. glutamicum, and cyanobacteria for advanced biofuels and biochemicals- Isoprenoid-based biofuels (Terpenes): Advanced bio-disel and aviation fuel (the next generation biofuels)- Value-added chemicals (>C3 chemicals) production from CO2- Biodiesel and alcohol production from renewable feedstock- Production of biochemicals replacing petroleum-based chemicals.- Production of biochemicals replacing plant extracts. (Cosmetics, pharmaceuticals)
2. Genetic tool development for metabolic engineering using synthetic biology- Systematic gene cloning using BglBrick and Golden Gate method- Systematic gene deletion and integration methods- CRISPR-CAS9 mediated genome editing and CRISPRi-mediated gene controlling- CRISPR-CAS9 mediated Base Editing- Various CRISPR-Cascade systems for genome engineering- Transcriptional and translational controls using RNAs- Genetic re-wiring of control units and biosensors- Its application to industrial host
3. Automatic Metabolic Engineering using Robot Platform(Biofoundry Platform)- High throughput strain development using Robot Platform- Bioengineering of Robot platform integrated with Genome Editing and RNA Editing- Lab. Adaptive Evolution for strain improvement using Robot Platform in the Biofoundry
4. Synthetic microbiome-Engineering of commensal bacteria for therapeutical drug delivery-Development of genetic circuit for commensal bacteria or engineered bacteria-Understanding of synthetic microbiome consortia
5. Systems biology with omics technologies- Genomics - Next generation genome sequencing technology- Transcriptomics - DNA microarray and Next generation seq. technology- Proteomics - MS-based targeted proteomics- Metabolomics - MS-based Metabolite profiling, targeted metablites- AI-based modeling
6. in silico flux balance analysis & Data-driven AI technologies- Genome-scale metabolic flux balance analysis- Prediction of metabolic behaviors for metabolic engineering and synthetic biology- AI-based modeling and prediction & wet demonstration
7. de Novo pathways to microbe-Biochemical productions from carbon dioxide using engineered Cyanobacteria-Deciphering de Novo pathway in C. glutamicum[Keywords]Microbial metabolomics,, Metabolic Profiling, Bio-RefineryComputational & Quantitative Biology, Systems Biology, Integrated Biology,Microbiology, Metabolic engineering, Secondary metabolite production, Drug Development, Diagnostics, Toxicology, Synthetic Biology, Bio-fuel
8. Regulations of cellular metabolisms and physiologies of E. coli and C. glutamicum- Characterization of two-component regulatory systems- in vitro or in situ validation of cellular regulations- Dynamic regulation of gene transcription and translation
2. Genetic tool development for metabolic engineering using synthetic biology- Systematic gene cloning using BglBrick and Golden Gate method- Systematic gene deletion and integration methods- CRISPR-CAS9 mediated genome editing and CRISPRi-mediated gene controlling- CRISPR-CAS9 mediated Base Editing- Various CRISPR-Cascade systems for genome engineering- Transcriptional and translational controls using RNAs- Genetic re-wiring of control units and biosensors- Its application to industrial host
3. Automatic Metabolic Engineering using Robot Platform(Biofoundry Platform)- High throughput strain development using Robot Platform- Bioengineering of Robot platform integrated with Genome Editing and RNA Editing- Lab. Adaptive Evolution for strain improvement using Robot Platform in the Biofoundry
4. Synthetic microbiome-Engineering of commensal bacteria for therapeutical drug delivery-Development of genetic circuit for commensal bacteria or engineered bacteria-Understanding of synthetic microbiome consortia
5. Systems biology with omics technologies- Genomics - Next generation genome sequencing technology- Transcriptomics - DNA microarray and Next generation seq. technology- Proteomics - MS-based targeted proteomics- Metabolomics - MS-based Metabolite profiling, targeted metablites- AI-based modeling
6. in silico flux balance analysis & Data-driven AI technologies- Genome-scale metabolic flux balance analysis- Prediction of metabolic behaviors for metabolic engineering and synthetic biology- AI-based modeling and prediction & wet demonstration
7. de Novo pathways to microbe-Biochemical productions from carbon dioxide using engineered Cyanobacteria-Deciphering de Novo pathway in C. glutamicum[Keywords]Microbial metabolomics,, Metabolic Profiling, Bio-RefineryComputational & Quantitative Biology, Systems Biology, Integrated Biology,Microbiology, Metabolic engineering, Secondary metabolite production, Drug Development, Diagnostics, Toxicology, Synthetic Biology, Bio-fuel
8. Regulations of cellular metabolisms and physiologies of E. coli and C. glutamicum- Characterization of two-component regulatory systems- in vitro or in situ validation of cellular regulations- Dynamic regulation of gene transcription and translation
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