식품생물공학실험

Date: 2016.9.1 updated

Instructor   

Instructor                                    Prof. Han Min Woo (우한민 교수님)

Teaching Assistant (TA)             김성은/김동재/최은빈

Department                                 Food Science and Biotechnology

Office location & hours                62-212, Wed. 2 pm - 4 pm or RSVP (Email: hmwoo@skku.edu)

Contact Information                    Tel: 031-290-7810, Email: Email: hmwoo@skku.edu

Class Hours                                Thr, 6:00 pm ~ 8:45 pm

Course Objectives and Outlines

Objectives:

The objective of the course, as implied by the course content above, is to introduce fundamental lab work of food bioprocess engineering concepts primarily to biochemical engineers and future scientist. The course does assume any biological background or required pre-courses in Lab experiment of Food Microbiology (FBT2012) or equivalent, Food Bioprocess Engineering (FBT2015) or equivalent. This course contains sections of laboratory experiments related to DNA recombinant Technologies, fermentation, production isolation, food-related bioprocess. Specifically, Lycopene, anti-oxidant chemical, can be produced from various carbon sources using engineered Escherichia coli (bacteria). Finally, purified lycopene will be expected.

Pre-requirement course

: 식품미생물학실험 (FBT2012) or equivalent.

: 식품생물공학 Food Bioprocess Engineering (FBT2015) or equivalent.

Text and supplementary books

Supplementary books

Text book: Shuler, M.L. and Kargi, F. Bioprocess Engineering, Basic Concepts. Second edition. 2002. Prentice-Hall Inc. 

Gene cloning & DNA analysis - An introduction six ed. T.A. Brown, Wiley-Backwell Press 2015. ISBN: 978-1-119-07256-0

Contents:

Class assignment

- Due by a week. Delay may influence the scores. (In the box at the 61-254)

- No word processing. Only hand writing acceptable.

Team assignment

- Group 3-5 students (The group participants will be notified)

- more than 2 pages : (Title, results, discussion, references) in English or Korean. No copy & paste (plagiarism => F grade). 

- Results and Discussion (including images, photo, figures with data points, or bar graph)

- Front page (excluding counting 4 pages): Title, Name, Affiliation, Date

- font: 11 pt, 1.5 line spacing

-

Evaluation

1. Attendance 20%, Discussion 5%, Attitude 5%, Assignment: (#8 items) 20%, Mid Term 25%, Final 25%, 

2. (F grade: Below 3/4 attendance of 15 weeks = <11.25; Four days absent (= 8 classes missing) ==> F graded 

3. Exam scores less than 6 pt out of 100 pt for each Mid-, Final regardless of all attendances ==> F graded 

Class dates

Class 01            06 Mar           Class outline & Intro - Lycopene production from sugar using engineered bacteria (Grouping)

Class 02            13 Mar           DNA recombinant Technology I - Vector (plasmid) and its isolation: mini-prep         (backbone & insert) [학부용]

Class 03            20 Mar           DNA recombinant Technology II - Plasmid digestion 

Class 04            27 Mar           DNA recombinant Technology III - Electrophoresis, Gel prep. and Ligation (Theory) 

Class 05            03 Apr           DNA recombinant Technology IV - Transformation (Heat shock)

Class 06            10 Apr            Strain development & Confirmation I - Colony PCR  -총선

Class 07            17 Apr           Strain development & Confirmation II-electrophoresis: cloning 확인, Trouble shooting : Theory (PCR, Ligation, Cloning methods.)

Class 08            24  Apr           Mid-Term (Optional: Food Camp.)

Class 09            01 May           Preparation for fermentation - Medium prep., Autoclave

Class 10            08 May           Fermentation: Pre-culture (02 May), Main culture, OD measurement (6pm, 9pm, 10pm, 11 pm, 9 am - 12 am), final sampling 

Class 11            15 May           - No class due to overnight work - 부처님오신날-

Class 12            22 May           대상 바이오연구소 견학 (5/30-5pm)

Class 13            29 Jun            Product analysis - GC/MS, HPLC (Theory) & Process design (Design), Fermentation-Scale up - Theory & Lab. 

Class 14            05 Jun            Production isolation - Acetone extraction & incubation

Class 15            12 Jun            Self-motivated studies or Make-up day

Class 16            19 Jun           Final Exam.

: pSe2Bb1k-LYCO4 => Bg-LYC04 -> pBbA5c-RFP, pBbE1c-RFP, pBbA1c-RFP

: pAC-LYC04 (control)

#notice 1: Organization of Term Project (Professor's official business due to the academic conference): TA attended

#notice 2: Team Project due (~20 min. Presentation) & Term Proposal (Final): email to hmwoo@skku.edu by 06 Dec. 

역대조교:

윤진경(2017)

최선영박사(2018)-고숭천

고숭천(2019)-손지경, 강석원

이미은(2020)-강두현/이동현

박재현(2021)-허유빈/(김필/조혜정)

손지경(2022)-노은지(짝수)/조세현(홀수)

허유빈(2023)-김성은/장혜원

김성은(2024)-김동재/최은빈

김동재(2025)-박준성/이유림