Chemical Biology

a) c-di-GMP signaling (biofilms) 

Thiazole Orange-Induced c-di-GMP Quadruplex Formation Facilitates a Simple Fluorescent Detection of This Ubiquitous Biofilm Regulating Molecule. Shizuka Nakayama, Ilana Kelsey, Jingxin Wang, Kevin Roelofs, Bogdan Stefane, Yiling Luo, Vincent Lee and Herman O. Sintim* J. Am. Chem. Soc. 2011, 133, 4856-4864.

C-di-GMP-proflavine-hemin supramolecular complex, has peroxidase activity- implication for a simple colorimetric detection. Shizuka Nakayama, Kevin G. Roelofs, Vincent T. Lee and Herman O.Sinim*, Mol. BioSyst. 2012, 8, 726-729.

b) Riboswitch

 Differential binding of 2’-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins. Yiling Luo, Jie Zhou, Sarah K. Watt, Vincent T. Lee, T. Kwaku Dayie and Herman O. Sintim*  Mol. BioSyst. 2012,  8, 772-778.

 Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy.Shizuka Nakayama, Yiling Luo, Jie Zhou, T. Kwaku Dayie and Herman O. Sintim* Chem. Commun. 2012, 48, DOI:10.1039/C2CC34379G 

c) Quorum sensing (virulence and Biofilms)  

 Altering the communication networks of multispecies microbial systems using a diverse toolbox of AI-2 analogs. Sonja Gamby, Varnika Roy, Min Guo, Jacqueline A. I. Smith, Jingxin Wang, Xiao wang, Jessica E Stewart, William E Bentley, and Herman O. Sintim* ACS Chem. Biol. 2012, 7, 1023-1030. (Selected as Cover for June 2012)

 AI-2 analogs and antibiotics: A synergistic approach to reduce bacterial biofilms.Varnika Roy, Mariana Meyer, Jacqueline Smith, Sonja Gamby, Herman O. Sintim, Reza Ghodssi, William Bentley, Appl. Microbiol. Biotechnol. 2012, in press.