Extracellular enzymes

Duhamel, S., Gregori, G., Van Wambeke, F., and Nedoma, J. Detection of Extracellular Phosphatase Activity at the Single-Cell Level by Enzyme-Labeled Fluorescence and Flow Cytometry: The Importance of Time Kinetics in ELFA Labeling. Cytometry Part A, 75A: 163–168, 2009 (IF= 2.978).

Štrojsová, A., Nedoma, J., Štrojsová, M., Cao, X., and Vrba, J. The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment. Journal of Limnology, 67: 128–138, 2008 (IF= 2.375).

Van Wambeke, F., Nedoma, J., Duhamel, S., Lebaron, P. Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea. Aquatic Microbial Ecology, 52: 245–251, 2008 (IF= 2.385).

Duhamel, S., Gregori, G., Van Wambeke, F., Mauriac, R., Nedoma, J. A method for analysing phosphatase activity in aquatic bacteria at the single cell level using flow cytometry. Journal of Microbiological Methods, 75: 269–278, 2008 (IF= 2.153).

Nedoma, J., Van Wambeke, F., Štrojsová, A., Štrojsová, M., Duhamel, S. Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments. Marine and Freshwater Research, 58: 454–460, 2007 (IF= 1.646).

Nedoma, J., García, J.-C., Comerma, M., Šimek, K., Armengol, J. Extracellular phosphatases in a Mediterranean reservoir: seasonal, spatial and kinetic heterogeneity. Freshwater Biology, 51: 1264–1276, 2006 (IF= 2.502).

Nedoma, J., Vrba, J. Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake. Environmental Microbiology, 8: 1271–1279, 2006 (IF= 4.630).

Štrojsová, A., Vrba, J., Nedoma, J., Šimek, K. Extracellular phosphatase activity of freshwater phytoplankton exposed to different in situ phosphorus concentrations. Marine and Freshwater Research, 56: 417-424, 2005 (IF= 1.478).

Vrba, J., Callieri, C., Bittl, T., Šimek, K., Bertoni, R., Filandr, P., Hartman, P., Hejzlar, J., Macek, M., Nedoma, J. Are bacteria the major producers of extracellular glycolytic enzymes in aquatic environments? International Review of Hydrobiology, 89 (1): 102-117, 2004 (IF= 0.742).

Nedoma, J., Padisák, J., Koschel, R. Utilisation of 32P-labelled nucleotide- and non-nucleotide dissolved organic phosphorus by freshwater plankton. Archiv für Hydrobiologie, Advances in Limnology, 58: 87-99, 2003 (no IF).

Štrojsová, A.,Vrba, J., Nedoma, J., Komárková, J., Znachor, P. Seasonal study of extracellular phosphatase expression in the phytoplankton of a eutrophic reservoir. European Journal of Phycology, 38: 295-306, 2003 (IF= 1.446).

Nedoma, J., Štrojsová, A., Vrba, J., Komárková, J., Šimek K. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Environmental Microbiology, 5: 462-472, 2003 (IF= 3.699).

Bittl, T., Vrba, J., Nedoma, J., Kopáček, J. Impact of ionic aluminium on extracellular phosphatases in acidified lakes. Environmental Microbiology, 3: 578-587, 2001 (no IF).

Vrba, J., Nedoma, J., Filandr, P. Resolving complex saturation kinetics: a potential tool in interpretation of extracellular β‑N-acetylhexosaminidase data from natural freshwaters. In: Muzzarelli, R.A.A. (ed): Chitin enzymology, Vol. 2. Atec Edizioni, 303–311, 1996 (no IF).

Vrba, J., Filandr, P., Nedoma, J., Šimek, K. Different sources of extracellular β‑N-acetylhexos-aminidase-like activities in freshwaters. In: Muzzarelli, R.A.A. (ed): Chitin enzymology, Vol. 2. Atec Edizioni, 293–301, 1996 (no IF).

Nedoma, J., Vrba, J., Hejzlar, J., Šimek, K., Straškrabová, V. N-acetylglucosamine dynamics in freshwater environments: Concentration of amino sugars, extracellular enzyme activities, and microbial uptake. Limnology and Oceanography, 39: 1088-1100, 1994 (IF= 2.151).

Vrba. J., Šimek, K., Nedoma, J., Hartman, P. 4-methylumbelliferyl-beta-N-acetylglucosaminide hydrolysis by a high-affinity enzyme, a putative marker of protozoan bacterivory. Applied and Environmental Microbiology, 59: 3091-3101, 1993 (IF= 3.128).

Vrba, J., Nedoma, J., Šimek, K., Seďa, J. Microbial decomposition of polymer organic-matter related to plankton development in a reservoir - activity of alpha-glucosidase, beta-glucosidase, and beta-N-acetylglucosaminidase and uptake of N-acetylglucosamine. Archiv für Hydrobiologie, 126: 193-211, 1992 (IF= 1.367).