Program: Human resources
Type of the project: Research projects for young research teams
This work was supported by a grant of the Romanian National Authority for Scientific Research, CNCS – UEFISCDI
Title: Efects of polyphenols in modulating the relationship between ErbB proteins and cell cycle progression in breast and epidermoid adenocarcinoma
Project number: PN-II-RU-TE-2011-3-0204
Contract: 111/10.10.2011
Duration: 36 months
Begining of the project: 10.10.2011
End of the project: 10.09.2014
Summary
ErbB proteins, members of the receptor tyrosine kinase (RTKs) family, and their signaling pathways play pivotal roles in migration, adhesion, apoptosis and proliferation of cells. In cancer cells the expression, interactions and signaling of ErbB proteins are often not subject to the tight regulation observed under physiologic conditions. Recent studies indicate that RTKs represent one of the critical targets of polyphenols in diminishing cancer cell proliferation. In the framework of our project we plan to investigate the effects of polyphenols on cell cycle progression and apoptosis in relation to the expression levels, interactions, activation state and signaling pathways of ErbB proteins in breast and epidermoid carcinoma cell lines. We will correlate these parameters with the types and concentrations of polyphenols. For our experiments we will generally use flow cytometry that provides robust statistical data. ErbB protein interactions will be investigated by both confocal microscopic immunofluorescent approaches (co-localization) and flow cytometric fluorescence resonance energy transfer. The activation state of ErbB proteins and their signaling pathways (e.g. PI3K/Akt kinase) will be investigated by western blotting. We expect that the project will clarify the role of polyphenols in influencing the ErbB receptor system and it will contribute to establishing the role of polyphenols in the treatment and chemoprevention of cancer.
Key words: ErbB proteins, polyphenols, tumor cells, cell cycle, apoptosis
Specific objectives
- Studying the impact of the polyphenols on the cell cycle progression, apoptosis and cell viability
- Investigating the effect of polyphenols on the expression level of ErbB proteins
- Studying the effects of polyphenols on the association state of ErbB proteins: interactions between ErbB proteins and β1-integrin or laminin receptor (67LR), ErbB1 homoassociation, ErbB2 homoassociation
- Investigating the correlation between the activation state of ErbB proteins and polyphenol treatments
- Investigating the effects of polyphenols on ErbB signalling pathways
Team:
Lecturer Dr. Mocanu Maria-Magdalena – principal investigator
Prof. Dr. Ganea Constanţa – senior researcher
Assoc. Prof. Dr. Irina Băran – postdoctoral researcher
Laura Georgescu – student
Flavia Iacobescu – student
Rodica Zaharia – technical assistant
Elena Constantin – technical assistant
Disemination of the results:
Publications:
- Mocanu Maria-Magdalena, Surcel Mihaela, Ursaciuc Cornel, Katona Eva, Ganea Constanta, Antiproliferative effect of quercetin in mammary and epidermoid cancer, ROMANIAN BIOTECHNOLOGICAL LETTERS, 2013, vol. 18, no. 6, pp. 8796-8803, (IF. 0.363)(is 0.115) [ link ]
- M.M. Mocanu, C. Ganea, L. Georgescu, T. Varadi, D. Shrestha, I. Baran, E. Katona, P. Nagy, J. Szöllösi, Epigallocatechin-gallate determines ErbB proteins downregulation, cell death mediated by 67kDa laminin receptor and altered lipid order in mammary and epidermoid carcinoma cells, 2013, J. NAT. PROD., DOI: 10.1021/np4007712
- Publication Date (Web): January 23, 2014, (IF. 3.285) (is. 1.867) [ link ]
- Mocanu MM, Ganea C, Siposova K, Filippi A, Demjen E, Marek J, Bednarikova Z, Antosova A, Baran I, Gazova Z, Polymorphism of lysozyme amyloid fibrils influences the cytotoxicity in LLC-PK1 epithelial kidney cells, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, Volume 65, April 2014, Pages 176-187, (if.2.596, is.0.618) [ link ]
National and international conferences:
- Mocanu MM, Nagy P, Georgescu L, Varadi T, Shrestha D, Baran I, Katona E, Szollosi J, Ganea C, The effect of flavonoids in mammary and epidermoid tumor cells with ErbB proteins overexpression, oral communication, 8th International Conference "Structure and Stability of Biomacromolecules" (SSB 2013), September 10-13, 2013, Kosice, Slovakia
- Mocanu MM, Ganea C, Siposova K, Fillipi A, Radu E, Demjen E, Marek J, Bednarikova Z, Antosova A, Gazova Z, Distinct fibrils morphology induces different cytotoxicity in renal cells 8th International Conference "Structure and Stability of Biomacromolecules" (SSB 2013), September 10-13, 2013, Kosice, Slovakia
- Gazova Z, Siposova K, Filippi A, Demjen E, Marek J, Ganea C, Mocanu MM, Polymorphism of lysozyme amyloid fibrils and cell viability, poster, 9th European Biophysics Congress (EBSA 2013), July 13-17, 2013 Lisbon, Portugal
- M.M. Mocanu, E. Katona, M. Surcel, L. Georgescu, T. Varadi, I. Baran, P. Nagy, C. Ursaciuc, J. Szöllősi, C. Ganea, Effect of flavonoids on cell cycle and apoptosis in tumor cell lines overexpressing ErbB proteins, oral communication, 9th National Congress of Cytometry, May 16-18, 2013, Bucharest, Romania
- Gazova Z., K. Siposova, M. Koneracka, V. Zavisova, A. Filippi, D. Ionescu, P. Kopcansky and M.M. Mocanu, Anti-amyloid effect of magnetic fluid in vitro and in cells, poster, 13th International Conference on Magnetic Fluid, January 7-11, 2013, New Dehli, India
- Gazova Z., Siposova K., Koneracka M., Zavisova V., Kopcansky P., Filippi A., Ganea C., Baran I., Mocanu M.M., Magnetic fluids attenuate the cytotoxic effect of amyloid fibrils, poster, 22nd IUBMB & 37th FEBS Congress, September 4 - 9, 2012, Sevilla, Spania
- M. M. Mocanu, K. Siposova, C. Ganea, I. Baran, A. Antosova, Z. Gazova, Protective role of nanoparticles against cytotoxic effect induced by amyloid fibrils, XXIII Biochemical Congress, August 26-29, 2012, Brno, Czech Republic
- M.M. Mocanu, C. Ganea, I. Baran, E. Katona, C. Ursaciuc, M. Surcel, P. Nagy, J. Szöllősi, Polyphenolic compounds induce the blockage of the cell cycle progression on tumor cell lines overexpressing ErbBs proteins, poster, ISAC XXVII International Congress, June 23 – 27, 2012, Leipzig, Germany
- M. M. Mocanu, K. Siposova, M. Muckova, J. Lazova, C. Ganea, I. Baran, Z. Gazova, Magnetic fluid reverses inhibition of cell growing caused by protein amyloid fibrils, 9th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, May 22-26, 2012, Minneapolis, Minnesota, U.S.A.
- A. Filippi, K. Siposova, A. Nedelcu, C. Ursaciuc, M. Surcel, A. Antosova, Z. Gazova, M.M. Mocanu, Lysozyme amyloid fibrils induce apoptosis on LLC-PK1 cells, poster, 8th National Citometry Congress, May 10 – 11, 2012, Bucharest, Romania
Latest updated: Jan 2014
This site is updated at the end of each significant research activity. The site's structure follows the CNCS guidelines (December 2011)
Contact information: Assoc. Prof. Dr. Maria-Magdalena Mocanu
UMF "Carol Davila" Bucharest, Disciplina de Biofizica
Bd. Eroii Sanitari nr 8
050474 Bucharest, Romania
email: mmocanu [at] umf [dot] ro