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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol- …
P Chomczynski, N Sacchi - Analytical biochemistry, 1987 - Elsevier
A new method of total RNA isolation by a single extraction with an acid guanidinium
thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation
of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful ...
Cited by 52143 - Related articles - BL Direct - All 20 versions
Volume 162, Issue 1, April 1987, Pages 156-159
doi:10.1016/0003-2697(87)90021-2 | How to Cite or Link Using DOI
Copyright © 1987 Published by Elsevier Inc.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Piotr Chomczynski, * and Nicoletta Sacchi†
†Laboratory of Molecular Oncology, NCI, National Institutes of Health, Frederick, Maryland 21701 USA
*Laboratory of Biochemistry and Metabolism, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 USA
Received 27 May 1986. Available online 7 December 2004.
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
Keywords: nucleic acids; RNA; messenger RNA; purification; gene expression; human cells