Asthma is one of the two leading chronic respiratory diseases (CRD) in the world. It affects almost 6 million children under the age of 18 in the United States alone. Vancomycin is an antibiotic that is used to treat serious infections when others fail. Previous research has shown that antibiotic intake at young age can lead to an increased rate of allergy development, alteration of the gut microbiota, and the development of asthma. Prenatal antibiotic exposure findings are still unclear with respect to the development of asthma in utero. Therefore, we hypothesize that Mice that receive Vancomycin prenatally will alter their microbiota and develop asthma. This study will be conducted over a six-week period. We will use Vancomycin on ill mice to monitor the development of asthma using 16sRNA sequencing. The goal is to establish which group was more affected by Vancomycin by developing asthma. The results will depict and support my hypothesis. We propose that future research should be tested in humans as this study was only conducted in mice.