Glioblastoma Multiforme is the most common type of malignant primary brain tumor and is currently incurable due to the heterogeneity of its tumor composition. Therefore, this study proposes to create an autobioluminescent cellular model in a 2D cell culture format using glioblastoma cells that would serve as the baseline for a future 3D cellular model. The cells in this study were patient-derived and pre-made to exhibit autonomous bioluminescence. In order to develop the cells to get a sufficient amount for the development of a cellular model, cell culture was performed on the cells every 3 days for 2 weeks. Then the cells were evaluated twice using a Varioskan Lux imaging machine. The evaluation demonstrated multiple problems with the cells and Varioskan Lux during these imaging trials. Therefore, this study suggests that there will need to be more experiments conducted in the future to better enhance the 2D cellular model's accuracy.