Selection and setting up of the required instrument
Acquisition, analysis and interpretation of the data
Cell sorting
Analysis vs Sorting
Analysis
Involves using a flow cytometer to interrogate cells stained with fluorescent markers and classifying them into groups. Cells pass through the instrument once and go straight to the waste.
There is no cell recovery.
Cell sorting
Involves using a flow cytometer with cell sorting capability to interrogate cells stained with fluorescent markers, classifying them into groups, and then physically separate the groups into test tubes or multi-well plates.
A fraction of the starting population defined by the gating strategy is recovered and the remainders of cells go to a waste container.