This project aims to gather interdisciplinary perspectives for a scientifically accurate and engaging serious game that immerses players in the connected complexities of the critically challenging topic of antimicrobial resistance (AMR) within food and water systems. Unlike typical AMR educational games—which often limit scope to antibiotic usage or require expert over- sight— this design would address broader One Health system challenges that are dynamic and require interdisciplinary perspectives, carefully selected with input from scholarly literature and expert collaborations. Since serious games often fall prey to complex exploration pathways – the design will also explore AI/ML techniques to identify and examine player strategies, providing feedback for players and enabling researchers to glean insights into collective decision-making and effective AMR stewardship. The design will be adaptable for use in both formal education, such as workforce development for healthcare professionals, and informal settings like museums and with youth development organizations like 4-H to raise public awareness. The project will 1) conduct a review of existing AMR games, 2) map systems thinking objectives to embed in game design, 3) assemble an interdisciplinary advisory group for iterative feedback, and 4) involve computer science students in advanced game prototyping. Future phases will pilot these prototypes with both experts and the general public, iteratively refining the games for scientific accuracy and widespread engagement. By combining adaptive technology and collaborative systems perspectives, this project offers transformative potential for public health education, policy development, and future AI-steered AMR interventions at the intersection of STEM, serious games, and One Health.