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Levitation Technology can replace FACS sorting for targeted viable cells of interest quickly and gently, ensuring the highest quality of cell health for use in downstream studies. FACS sorting is often rate-limiting in terms of sample number, requires skill to set up and run, and requires labeling of cells, which can also increase the overall workflow time.
Levitation Technology can be a complementary tool used upstream of flow cytometry to clean up the sample and drastically improve flow cytometry analytical results, and speed.
Levitation Technology can be a complementary tool used downstream of cell sorting to rescue the healthiest, viable cells after the cell sorting process. During the sorting process, cells are subject to high pressure, electrostatic charge changes, and flight into collection vessels. All of which can injure or perturb cells in negative ways, and the LeviCell can recover the quality of the sample.
Marketing Content
Handbook -
Flow Cytometry Handbook Rev A - New content!
Training Slides, Battle Cards
Flow Cytometry Training Slides- General (Mark Barnes)
Flow Cytometry Training Slides- Cell Sorting (Melissa Ma)
Research Snapshot
Background Information
Flow Cytometry is the analysis method for detecting and measuring properties or characteristics of a single cell. Cells are typically labeled with a fluorescently conjugated probe or unlabeled using scatter properties. Cells are hydrodynamically focused in a stream into a single file. As each cell passes through an interrogation point, a light source like a laser is used to excite the fluorophores used to mark the cell. Detectors then capture the emitted light and measurements are translated into data. In this analysis mode, cells go to waste after interrogation.
Cell Sorting uses flow cytometry techniques and additional hardware features to not only interrogate the cell but also track its position in the stream. The cell sorter hardware contains a means to break the stream into droplets or segregate the stream into partitions. The individual cells are precisely broken into droplets where they can be deflected using electrostatic charge into different collection tubes or are selectively moved into a different channel away from unwanted cells. Cells that have the correctly identified properties will be retained and used in down stream studies.
Common brands for flow cytometers and cell sorters are:
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