General Applications

Stamp

Stamp 96-to-96

  • Method Status: Available on FX & FXp

  • This method is useful for transferring all or some contents from one plate into another plate. The plate types can differ or be identical. For every source plate, there must be a destination plate.

  • Maximum deck capacity: 5 source and destination pairs

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): PCR setup

Stamp Res-to-96

  • Method Status: Available on FX & FXp

  • This method is useful for dispensing different reagents in bulk (200uL increments per channel) to prepare multiple plates. For every source reservoir, there must be an associated destination plate.

  • Maximum deck capacity: 5 source and destination pairs

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): Culture Prep

Stamp 384-to-384

  • Method Status: Available on FX

  • This method is useful for transferring all or some contents from one plate into another plate. The plate types can differ or be identical. For every source plate, there must be a destination plate.

  • Maximum deck capacity: 2 source and destination pairs

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): Echo Prep

One to Many

One to Many 96-to-96

  • Method Status: Available on FX & FXp

  • This method is useful for dispensing aliquots from one plate into multiple destination plates.

  • Maximum deck capacity: 1 source and 13 destinations

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): PCR setup

One to Many Res-to-96

  • Method Status: Available on FX & FXp

  • This method is useful for dispensing reagent in bulk (200uL increments per channel) to prepare multiple plates.

  • Maximum deck capacity: 1 source and 13 destinations

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): Media prep

Combination

Combination 96-to-384

  • Method Status: Available on FX & FXp

  • This method is useful for combining four different 96-well plates of the same type into one 384-well plate.

  • Maximum deck capacity: 1 source and 4 destinations

  • Equipment: Biomek FX, Biomek FXp

  • Example(s): Pooling

Combination 24-to-96

  • Method Status: In Progress

  • This method is useful for combining four different 24-well plates of the same type into one 96-well plate.

  • Maximum deck capacity: 1 source and 4 destinations

  • Equipment: Biomek NXp, Biomek NX-S8

  • Example(s): Pooling

Breakdown

Breakdown 384-to-96

  • Method Status: Available on FX & FXp

  • This method is useful for splitting any or all quadrants of a 384-well plate into multiple 96-well plates of the same type.

  • Maximum deck capacity: 1 source and 4 destinations

  • Equipment: Biomek FX, Biomek FXp

  • Example(s):

Breakdown 96-to-24

  • Method Status: In Progress

  • This method is useful for splitting any or all quadrants of a 96-well plate into multiple 24-well plates of the same type.

  • Maximum deck capacity: 1 source and 4 destinations

  • Equipment: Biomek NXp, Biomek NX-S8

  • Example(s):

Serial Dilution

Serial Dilution 96-to-96

  • Method Status: Available on FX

  • This method is useful for performing a stepwise dilution of a source liquid/solution into different destination plates where the diluted material of the first destination plate is used as the source for the dilution of the next destination plate.

  • Maximum deck capacity: 1 source and 13 destinations

  • Equipment: Biomek FX, Biomek FXp

Serial Dilution Res-to-96

  • Method Status: Available on FX

  • This method is useful for performing a stepwise dilution of a source liquid/solution into different destination plates where the diluted material of the first destination plate is used as the source for the dilution of the next destination plate.

  • Maximum deck capacity: 1 source and 13 destinations

  • Equipment: Biomek FX, Biomek FXp

Qtray Plating

Qtray Plating

  • Method Status: Available on FXp, NXp, and NX-S8

  • This method is particularly useful for preparing 48-well Qtrays by plating (dispensing) cell culture onto a solid agar. Half of the source plate is transferred onto one Qtray while the other half is plated on another Qtray. Once those cells grow into colonies, the Qtrays are then transferred onto the Molecular Devices Qpix for colony picking.

  • Maximum deck capacity: 1 source and 2 Qtrays

  • Equipment: Biomek FXp, Biomek NXp, Biomek NX-S8

  • Example(s): Transformation plating setup

Hitpick

Hitpick

  • Method Status: In Progress

  • This method is useful for transferring any specific well of one plate into another well of another plate. Plate formats (i.e. number of wells) do not have to match from source to destination. Typically, inter-plate transfers are done, but intra-plate transfers can be accomplished as well.

  • Transfers can follow a user-specified pattern in a worklist or be done at random.

  • Equipment: Biomek FXp, Biomek NXp, Biomek NX-S8

  • Maximum deck capacity: Variable (if want new tips for every transfer, can have 4 sources and 4 destinations; otherwise, can have any combinations of 11 total sources and destinations combined)

  • Example(s): Archiving

For more information, please email ese-robotics@lbl.gov