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Content Objective: To explore how chromatography works and how it can be used.
Language Objective: To describe how the Rf value is calculated and use it to determine a pigment.
Book Reference: NA
Syllabus Details:
Separation of photosynthetic pigments by chromatography
Drawing an absorption spectrum for chlorophyll and an action spectrum for photosynthesis
Visible light has a range of wavelengths with violet the shortest wavelength and red the longest
Chlorophyll absorbs red and blue light most effectively and reflects green light more than other colours
Background
Background
Pigments are molecules that absorb light
Chlorophyll a and Chrolophyll b are examples
Xanthophyll and carotenes are other pigments that also absorb light in photosynthetic organisms
Chromatography is an experimental technique by which mixtures can be separated
A mixture is dissolved in a fluid (this can vary depending on the material).
The mixture is then separated by adding the mixture to a solvent (usually).
The pigments are separated by based on how quickly they move through the solution
A Rf value is calculated
Rf value = distance component travels/distance solvent travels
Data
Data
Resources
Resources
Absorption Spectrum
Absorption Spectrum
Engelmann's Algae Experiment
Engelmann's Algae Experiment
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