Glyphosate, also known as isopropylamine salt, is the active ingredient in Roundup and many other herbicides. The use of Roundup has grown dramatically since the product™s release in 1974 due to Glyphosate resistant and genetically modified plants and weeds. This increased usage has led to glyphosate-based herbicides being oversprayed. Overspraying herbicides comes with consequences such as soil contamination, polluted air, chemical residue on food and contaminated drinking water. The purpose of this study is to determine if Glyphosate has negative health effects to aquatic invertebrates which carry similar organ systems as vertebrates such as fish and frogs. Girardia tigrina are going to be exposed to a range of concentrations from 0 mg/L to 15 mg/L while testing their natural light escape abilities. The data collected showed that planarians' light escape times are slower in the lowest concentrations 1.87 mg/L and 3.75 mg/L than in the higher concentrations 7.5 mg/L and 15 mg/L. Escape times collected were also higher in post-bisection trials and higher overall in the 41% Glyphosate Grass and Weed Killer compared to post-bisection trials in Roundup.