PFOA and PFOS were looked at using mayflies (Ephemeroptera), stoneflies (Plecoptera) and caddisflies (Trichoptera) as biomarkers in the Hoosic River. PFC’s are synthetic, chemical compounds that are persistent in the environment. This study aims to determine if there is a gradient effect of PFOA and PFOS in the Hoosic River. Water samples were collected prior to live tissue samples. Samples were collected from 5 sites on the Hoosic River and one reference site in Lake George. The water contamination was compared to the levels of PFC’s in the aquatic insects. The highest PFOA concentration in water was found to be 41.5 ng/L and the lowest concentration in water was found to be 1 ng/L. However, the highest concentration of PFOA and PFOS in tissue samples was found to be 7.8 ng/g and 20 ng/g, respectively. The lowest level for PFOS in tissues was found to be 5.95ng/g. The gradient for contamination in water differed from the gradient in the tissue samples. The health effects of PFC contamination are inconclusive, therefore more studies must be done to determine what the contamination means to the residents of Hoosick Falls and surrounding areas. Research supported by Marjot Foundation.