植物の分子育種に関わる研究 

お知らせ

2025年9月11日　受賞式に行って来ました。　本学HPにも載せてもらっています。

　　　　　　　　　写真は以下のurLをご覧ください。

2025年8月20日　日本植物バイオテクノロジー学会の学会賞(技術賞）受賞について、

プレスリリースを行いました。
受賞タイトル「分子育種技術を駆使した新奇性の高いリンドウ品種の育成」

記事はこちらから。

2025年8月12日

日本植物バイオテクノロジー学会 第８回産学官協力セミナー  「これまでにない花の色−バイオテクノロジーで新品種—」

西原昌宏　（福井県立大学）　動画が公開されました。

https://youtu.be/Emqkd-kyBX4?si=ltzZrf9ID2BOZRTO

2025年7月24日

リンドウの臭い香り成分の生合成に関する遺伝子単離の論文を発表しました。分岐鎖アミノ酸アミノ基転移酵素2種類です。

Teshima et al. Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora.. Planta 262: 62 (link)

2025年7月18日

重イオンビームによるリンドウの突然変異に関する論文を発表しました。　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Nishihara et al. Mutagenesis and production of double-flowered gentian by regeneration from ion-beam irradiated leaves. 　　　　　　　　　　　　　　　　　Plant Biotechnology (in press) 

岩手生工研からプレスリリースもしています。

2025年7月1日　日本植物バイオテクノロジー学会の学会賞(技術賞）を受賞することになりました。

授賞式は2025年9月、神戸にて開催される本年度大会で行われます。

 ◯西原 昌宏 1、根本圭一郎 2、小田島雅 3、高橋亮 4、下川卓志 5　

(◯代表者  1 福井県立大学、2 岩手生物工学研究センター、3 岩手県農研センター、4 八幡平市花き研究開発センター、5 量子医科学研究所)
受賞タイトル「分子育種技術を駆使した新奇性の高いリンドウ品種の育成」

2025年6月23日　リンドウの越冬芽で発現するOil Body-Associated Protein 1(OBP1)に関する論文を発表しました。

2025年6月　夢ナビ講義Videoに出演しました。　ぜひ、ご覧下さい。

　　　　　　タイトル「遺伝子の力を借りて、花の色や形をデザインする！」

2025年4月 学科長に就任しました。

2024年10月　3年生　2名が分属されました。

2024年7月 オープンキャンパスの教員紹介の動画を公開しました。

2024年6月28日 ベタシアニンによるトレニアの花色改変の論文を発表しました。

本学のHPにも掲載いただきました。

2023年10月　3年生1名が分属されました。研究室の立ち上げで大変ですが、卒論ぬ向けて頑張りましょう。

2023年10月1日に着任しました。よろしくお願いします。

発表論文

2025

1. Mutagenesis and production of double-flowered gentian by regeneration from ion-beam irradiated leaves. Nishihara, M., Hirabuchi, A., Abe, A., Shimizu, M., Goto, F., Yoshida, C., Shimokawa, T., Ozawa, S., Naito, A., Nemoto, K.  Plant Biotechnology (in press) 

2. Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora. Teshima, T., Nemoto, K., Motoki, S., Yoshida, C., Hirabuchi, A., Goto, F., Odashima, M., Naito, Z., Nishihara, M. Planta 262: 62  (link)

3. Gentian homologue of OIL BODY-ASSOCIATED PROTEIN1 is the key to a novel mechanism of growth recovery from cold stress via triacylglycerol hydrolysis. Takahashi, H., Yamada, H., Shimizu, M., Yoshida, C., Nishihara, M., Itoh. K. .Plant Science 359: 112616s (link)

2024

1. Flower color modification in Torenia fournieri by genetic engineering of betacyanin pigments. Nishihara, M.,  Hirabuchi, A., Teshima, T., Uesugi, S., Takahashi, H. BMC Plant Biology  24: 614 (link)

着任以前の論文

2023

1.  Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta. Atsumi, G., Naramoto, S., Nishihara, M., Nakatsuka, T., Tomita, R., Matsushita, Y., Hoshi, N., Shirakawa, A., Kobayashi, K., Fukuda, H., Sekine, K.T.  Journal of Virology  97: e0046323 (link)

2. Efficient double-flowered gentian plant production using the CRISPR/Cas9 system. Nishihara, M., Hirabuchi, A., Goto, F., Watanabe, A., Yoshida, C., Washiashi, R., Odashima, M., Nemoto, K.   Plant Biotechnology 40: 229-236 (link) 

3.  Preface to the special issue “Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology”.  Nishihara, M. and Muranaka, T. Plant Biotechnology 40:181-184 (link) 

4. Production of yellow-flowered gentian plants by genetic engineering of betaxanthin pigments. Nishihara, M., Hirabuchi, A., Goto, F., Nishizaki, Y., Uesugi, S., Watanabe, A., Tasaki, K., Washiashi, R., Sasaki, N. New Phytologist 240: 1177-1188 (link)

5. Metabolic engineering of betacyanin in vegetables for anti-inflammatory therapy.   Saito, S., Nishihara, M., Kohakura, M.,  Kimura, K.,  Yashiro, T., Takasawa, S.. Arimura, G.  Biotechnology and Bioengineering 120: 1357-1365 (link)

2022

1. De novo transcriptome analysis reveals flowering-related genes that potentially contribute to flowering-time control in the Japanese cultivated gentian Gentiana triflora. Takase, T., Shimizu, M., Takahashi, S., Nemoto, K., Goto, F., Yoshida, C., Abe, A., Nishihara, M.  International Journal of Molecular Sciences 23: 11754 (link)

2. Methods to promote seed germination in the lacquer tree, Toxicodendron vernicifluum (Stokes) F.A. BarkleyNemoto, K., Watanabe, A., Yoshida, C., Nishihara, M. PLoS ONE 17: e0272665 (link)

3. Genome analysis revives a forgotten hybrid crop Edo-dokoro of the genus Dioscorea.  Natsume, S., Sugihara, Y., Kudoh, A., Oikawa, K., Shimizu, M., Ishikawa, Y., Nishihara, M., Abe, A., Innan, H., Terauchi, Plant & Cell Physiology 63: 1667–1678 (link)

4. Identification of candidate genes responsible for flower colour intensity in Gentiana triflora. Tasaki, K., Watanabe, A., Nemoto, K., Takahashi, S., Goto, F., Sasaki, N., Hikage, T., Nishihara, M.  Frontiers in Plant Science 13: 906879 (link).

5. Isolation and functional analysis of EPHEMERAL1-LIKE (EPH1L) genes involved in flower senescence in cultivated Japanese gentians.    Takahashi, S,   Yoshida, C.,   Takahashi, H., Nishihara, M.  International Journal of Molecular Sciences 23, 5608  (link) .

6. Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.   Nemoto, K., Niinae, T., Goto, F., Sugiyama, N., Watanabe, A., Shimizu, M., Shiratake, K., Nishihara, M. The Plant Cell 34: 2652–2670 (link)

7. Gentian FLOWERING LOCUS T orthologs regulate phase transitions: floral induction and endodormancy release. Takahashi, H., Nishihara, M., Yoshida, C., Itoh, K.   Plant Physiology 188: 1887-1899 (link)

8. ひとりではじめる　植物バイオテクノロジー入門　～組織培養からゲノム編集まで～「リンドウの形質転換およびゲノム編集」pp212-223　西原昌宏、国際文献社 (link)

9. ｢日本園芸リンドウにおけるゲノム編集技術の利用の現状と展望｣　西原昌宏  ゲノム編集作物：花卉の開発と新展開  月刊アグリバイオ増2022年８月号　北隆館 (link)　

2021

1. Post-transcriptional gene silencing of chalcone synthase gene CHS causes corolla lobe-specific whiting of Japanese gentian.  Ohta, Y., Atsumi, G., Yoshida, C., Takahashi, S., Shimizu, M. Nishihara, M., Nakatsuka, T.  Planta 255: 29 (link)

2. Identification and characterization of xanthone biosynthetic genes contributing to the vivid red coloration of red-flowered gentian. Sasaki, N., Nemoto, K., Nishizaki, Y., Sugimoto, N., Tasaki, K., Watanabe, A., Goto, F., Higuchi, A., Morgan, E., Hikage, T., Nishihara, M.  Plant Journal 107: 1711-1723  (link) 

3. Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants. Koeduka, T., Takarada, S., Fujii, K., Sugiyama, A., Yazaki, K., Nishihara, M., Matsui, K.  Metabolic Engineering Communications 13: e00180 (link)

4. Additional betalain accumulation by genetic engineering leads to a novel flower color in lisianthus (Eustoma grandiflorum).   Tomizawa, E., Ohtomo, S., Asai, K., Ohta, Y., Takiue, Y., Hashumi, A., Nishihara, M., Nakatsuka, T.  Plant Biotechnology 38: 323-330 (link)

2020

1. Esterified carotenoids are synthesized in petals of carnation (Dianthus caryophyllus) and accumulate in differentiated chromoplasts.Iijima, L., Kishimoto, A., Ohmiya, A., Yagi, M., Okamoto, E., Miyahara, T., Tsujimoto, T., Ozeki, Y., Uchiyama, N., Hakamatsuka, T., Kouno, T., Cano, E.A., Shimizu, M.,  Nishihara, M. Scientific Reports 10: 15256 (link)

2. Morphological and cytological observations of corolla green spots reveal the presence of functional chloroplasts in Japanese gentian. Takahashi, S., Ozawa, S., Sonoike, K., Sasaki, K., Nishihara, M.   PLoS ONE 15: e0237173 (link) 

3. Molecular characterization of an anthocyanin-related glutathione S-transferase gene in Japanese gentian with the CRISPR/Cas9 system.  Tasaki, K., Yoshida, M., Nakajima, M., Higuchi, A., Watanabe, A., Nishihara, M.  BMC Plant Biology  20: 370 (link)

4. 高橋重一、西原昌宏 ｢新発見！リンドウは花弁の緑色斑点で光合成する｣ 学会誌 光合成研究 Vol30 (No3) 157-165.(link)

2019

1. Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers.  Tasaki, K., Higuchi, A., Watanabe, A., Sasaki, N., Nishihara, M. Scientific Reports 9: 15831 (link)

2. Inhibition of post-transcriptional gene silencing of chalcone synthase genes in Petunia picotee petals by fluacrypyrim.     Ban, Y., Morita, Y., Ogawa, M., Higashi, K., Nakatsuka, T., Nishihara, M., Nakayama, M. Journal of Experimental Botany 70: 1513–1523 (Link)

3. The host stomatal density determines resistance to Septoria gentianae in Japanese gentian. Tateda, C., Obara, K., Abe, Y., Sekine, R., Nekozuka, S., Hikage, T., Nishihara, M., Sekine, K., Fujisaki, K.  Molecular Plant-Microbe Interactions 32: 428-436 (Link)

4.  Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum. Hamachi, A., Nishihara, M., Saito, S., Rim, H., Takahashi, H., Islam, M., Uemura, T., Ohnishi, T., Ozawa, R., Maffei, M.E., Arimura, G. Planta 249: 235-249 (Link)

2018

1. Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri. Nishihara, M., Higuchi, A.,  Watanabe, A., Tasaki, K. BMC Plant Biology 18:331(Link) 

2. Biological effects of ion beam irradiation on perennial gentian and apple. Sasaki, N., Watanabe, A, Asakawa, T., Sasaki, M., Hoshi, N., Naito, Z., Furusawa, Y., Shimokawa, T., Nishihara, M. Plant Biotechnology 35: 249-257 (Link)

3. Carnation I locus has two chalcone isomerase genes involved in orange flower. Miyahara, T., Sugishita, N., Ishida-Dei, M., Okamoto, E, Kouno, T., Cano, E.A., Sasaki, N., Watanabe, A., Tasaki, K., Nishihara, M., Ozeki, Y.  Breeding Science 68: 481-487 (Link) 

4. 植物のたくらみ ー香りと色の植物学」有村源一郎・西原昌宏  ベレ出版  (Link)

5. Development of basic technologies for improvement of breeding and cultivation of Japanese gentian.  Nishihara, M., Tasaki, K., Sasaki, N., Takahashi, H. Breeding Science 68: 14–24 (Link)

6.  Repressed expression of a gene for a basic helix-loop-helix protein causes a white flower phenotype in carnation. Totsuka, A., Okamoto, E., Miyahara, T., Kouno, T., Cano, E.A., Sasaki, N., Watanebe, A., Tasaki, K. Nishihara, M., Ozeki, Y. Breeding Science 68: 139–143 (Link)

2017

1. Development of molecular markers for breeding of double flowers in Japanese gentian. Tasaki, K.,  Higuchi, A.,  Fujita, K., Watanabe, A., Sasaki, N.,  Fujiwara, K.,  Abe, H.,  Naito, Z., Takahashi, R., Hikage, T. and  Nishihara, M.  Molecular Breeding 37:33 [link] 

2. The 64-bp sequence containing GAAGA motif is essential for CaMV-35S promoter methylation in gentian.  Shimada, A., Okumura, A., Iwata, Y., Koizumia, N., Nishihara, M., Mishiba, K.  BBA - Gene Regulatory Mechanisms 1860: 861-869  [link] 

2016

1. Development of a Broad bean wilt virus 2-based expression vector for gentian. Tasaki,K., Atsumia,G, Nishihara, M. and Sekine, K. Scientia Horticulturae 201:279–286 [link] 

2. Functional characterization of duplicated B-class MADS-box genes in Japanese gentian. Nakatsuka, T., Saito, M. and Nishihara, M. Plant Cell Reports 35: 895-904  [link] 

3. CaMV-35S promoter sequence-specific DNA methylation in lettuce. Okumura, A.,   Shimada, A., Yamasaki, S., Horino, T., Iwata, Y., Koizumi, N., Nishihara, M., Mishiba, K. Plant Cell Reports 35: 43-51 [link] 

4. Metabolite profiling reveals the involvement of aberrant metabolic changes in Gentiana triflora seed showing poor germination. Takahashi, H., Fujita, K., Yoshida, C., Nishihara, M.  J. Hort. Sci. Biotech. 91:148–155 [link] 

2015

1. Molecular Breeding of Japanese Gentians— Applications of Genetic Transformation, Metabolome Analyses, and Genetic Markers. Nishihara, M., Mishiba, K., Imamura, T., Takahashi, H., Nakatsuka, T. In The Gentianaceae - Volume 2: Biotechnology and Applications. Springer.[link]

2. Isolation and characterization of the C-class MADS-box gene involved in the formation of double flowers in Japanese gentian. Nakatsuka, T, Saito, M., Yamada, Y., Fujita, K., Yamagishi, N., Yoshikawa, N., Nishihara, M. BMC Plant Biology 15:182 [Open Access]

3. Gentiobiose feeding in gentian in vitro overwintering buds or plantlets. Takahashi, H., Nishihara, M. Bio-protocol Bio-protocol. 5(12), e1499. [link]

4. Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora. Sasaki, N., Nishizaki, Y., Yamada, E., Tatsuzawa, F., Nakatsuka, T. Takahashi, H., Nishihara, M. FEBS Letters 589: 182-187 [link]

2014

1. Molecular characterization of mutations in white-flowered torenia plants. Nishihara, M., Yamada, E., Saito, M., Fujita, K., Takahashi, H., Nakatsuka, T. BMC Plant Biology 14: 86. [Open Access]

2. Silencing of DS2 aminoacylase-like genes confirms salicylic acid-dependent resistance to Phytophthora infestans in Nicotiana benthamiana. Nakano, M., Nishihara, M., Yoshioka, H., Ohnishi, K., Hikichi, Y., Kiba, A. Plant Signaling & Behavior 9: e28004 (link)

3. 植物の香りと色の代謝工学が拓く新時代. 有村源一郎・西原昌宏・下田武志 バイオサイエンスとインダストリー Vol.72 No.3 197-202. (link)

4. Mitsunami, T., Nishihara, M., Galis, I., Alamgir, KM., Hojo, Y., Fujita, K., Sasaki, N., Nemoto, K., Sawasaki, T., Arimura, G. (2014) Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura. PLoS ONE 9: e108849. [PLoS One LINK]

5. The gentio-oligosaccharide gentiobiose functions in the modulation of bud dormancy in the herbaceous perennial Gentiana. Takahashi, H., Imamura, T., Konno, N., Takeda, T., Fujita, K., Konishi, T., Nishihara, M., Uchimiya, H. Plant Cell 26:3949-3963 [link]

6. Transcriptional regulators of flavonoid biosynthesis and their application to flower color modification in Japanese gentians. Nakatsuka, T., Sasaki, N., Nishihara, M. Plant Biotechnology 31: 389-399 [link]

2013

1. Efficient haploid and double haploid production from unfertilized ovule culture of gentians (Gentiana spp.). Doi, H., Hoshi, N., Yamada, E., Yokoi, S., Nishihara, M., Hikage, T. and Takahata, Y. Breeding Science 63: 400-406. [Open Access]

2. Heterologous expression of gentian MYB1R transcription factors suppresses anthocyanin pigmentation in tobacco flowers. Nakatsuka, T., Yamada, E., Saito, M., Fujita, K. and Nishihara, M. Plant Cell Reports 32: 1925-1937. [Springer LINK]

3. Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana. Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y. and Kiba, A. PLoS One 8: e75124. [PLoS One LINK]

4. Metabolic engineering of the C16 homoterpene TMTT in Lotus japonicus through overexpression of (E,E)-geranyllinalool synthase attracts generalist and specialist predators in different manners. Brillada,C. Nishihara, M., Shimoda, T., Garms, S., Boland, W., Maffei, ME. and Arimura, G. New Phytologist 200: 1200-1211. [Wiley link]

5. Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering. Masahiro Nishihara, Takeshi Shimoda, Takashi Nakatsuka, Gen-ichiro Arimura. Plant Methods 9: 23. [Open Access]

6. Genetic engineering of yellow betalain pigments beyond the species barrier. Nakatsuka, T., Yamada, E., Takahashi, H., Imamura, T., Suzuki, M., Ozeki, Y., Tsujimura, I., Saito, M., Sakamoto, Y., Sasaki, N. and Nishihara, M. Scientific Reports 3: 1970. [Open Access]

7. Inheritance of brown leaf spot disease resistance in gentians. Nekoduka, S., Horaguchi, H., Akasaka, S., Chiba, K., Hikage, T., Kawamura, H., Nakatsuka, T. and Nishihara, M. Journal of General Plant Pathology 79: 165-167. [Springer Link]

8. Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome. Kobayashi, K., Atsumi, G., Iwadate, Y., Tomita, R., Chiba, K., Akasaka, S., Nishihara, M., Takahashi, H., Yamaoka, N., Nishiguchi, M. and Sekine, K. Journal of General Plant Pathology 79: 56-53 [Springer Link]

2012

1. Construction of the first genetic linkage map of Japanese gentian (Gentianaceae). Nakatsuka T, Yamada E, Saito M, Hikage T, Ushiku Y and Nishihara M. BMC Genomics 13: 672. [Open Access]

2. Isolation and characterization of GtMYBP3 and GtMYBP4, orthologues of R2R3-MYB transcription factors that regulate early flavonoid biosynthesis, in gentian flowers. Nakatsuka T, Saito M, Yamada E, Fujita K, Kakizaki Y, Nishihara M. Journal of Experimental Botany 63: 6505-6517. [PubMed]

3. Acquired immunity of transgenic torenia plants overexpressing agmatine coumaroyltransferase to pathogens and herbivore pests. Muroi, A., Matsui, K., Shimoda, T., Kihara, H., Ozawa, R., Ishihara, A., Nishihara, M. and Arimura, G. Scientific Reports 2: 689. [Open Access]

4. Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants. Komori, D., Nishihara, M., Takahashi, A., Gupta, M., Yoshioka, H., Mizumoto, H., Ohnishi, K. and Hikichi, Y. and Kiba, A. Plant Biotechnology 29: 293-300.

5. Plant-plant-plant communications, mediated by (E)-β-ocimene emitted from transgenic tobacco plants, prime indirect defense responses of lima beans. Arimura, G., Muroi, A., Nishihara, M. Journal of Plant Interactions. 7: 193-196. [Abstract]

6. The effect of genetically enriched (E)- β-ocimene and the role of floral scent in the attraction of the predatory mite Phytoseiulus persimilis to spider mite-induced volatile blends of torenia. Shimoda, T., Nishihara, M., Ozawa, R., Takabayashi, J., Arimura, G. New Phytologist 193: 1009-1021. [PubMed]

7. Gentian lipid transfer protein homolog with antimicrobial properties confers resistance to Botrytis cinerea in transgenic tobacco. Kiba, A., Nakatsuka, T., Yamamura, S., Nishihara, M. Plant Biotechnology 29: 95-101. [Abstract]

8. Development of DNA markers that discriminate between white- and blue-flowers in Japanese gentian plants. Nakatsuka, T., Saito, M., Sato-Ushiku, Y., Yamada, E., Nakasato, T., Hoshi, N., Fujiwara, K., Hikage, T. and Nishihara, M. Euphytica 184: 335-344. [Springer Link]

2011

1. A single-base substitution suppresses flower color mutation caused by a novel miniature inverted-repeat transposable element in gentian. Nishihara, M., Hikage, T., Yamada, E. and Nakatsuka, T. Molecular Genetics and Genomics 286: 371-382. [PubMed]

2. The composite effect of transgenic plant volatiles for acquired immunity to herbivory caused by inter-plant communications. Muroi, A., Ramadan, A., Nishihara, M., Yamamoto, M., Ozawa R., Takabayashi, J. and Arimura, G. PLoS One 6(10): e24594. [PLoS One LINK]

3. Development of simple sequence repeat markers for identification of Japanese gentian cultivars. Sato-Ushiku, Y., Shimada, N., Saito, M., Yamada, E., Hikage, T., Nakatsuka, T. and Nishihara, M. Journal of Japanese Society for Horticultural Science 80: 475-485. [Abstract]

4. Gynogenesis in gentians (Gentiana triflora, G. scabra) : production of haploids and doubled haploid. Doi, H., Yokoi , S., Hikage, T., Nishihara , M., Tsutsumi, K. and Takahata, Y. Plant Cell Reports 30:1099-1106. [PubMed]

5. The gentian orthologues of FT/TFL1 gene family control floral initiation in Gentiana. Imamura, T., Nakatsuka, T., Higuchi, A., Nishihara, M. and Takahashi, H. Plant and Cell Physiology 52: 1031-1041. [PubMed]

6. Production of picotee-type flowers in Japanese gentian by CRES-T. Nakatsuka, T., Saito, M., Yamada, E. and Nishihara, M. Plant Biotechnology 28: 173-180. [J-STAGE]

7. A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian. Kimura, S., Nakatsuka, T., Yamada, E., Saito , M. and Nishihara, M. Plant Biotechnology 28: 211-221. [J-STAGE]

8. De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian. Yamasaki, S., Oda, M., Koizumi, N., Mitsukuri, K., Johkan, M., Nakatsuka, T., Nishihara, M. and Mishiba, K. Plant Biotechnology 28: .223-230. 2011. [J-STAGE]

9. Epigenetic modifications of the 35S promoter in cultured gentian cells. Yamasaki, S. Oda, M., Daimon, H., Mitsukuri, K., Johkan, M., Nakatsuka, T., Nishihara, M. and Mishiba, K. Plant Science 180: 612-919. [PubMed]

10. The new FioreDB database provides comprehensive information on plant transcription factors and phenotypes induced by CRES-T in ornamental and model plants. Mitsuda, N., Takiguchi, Y., Shikata, M., Sage-Ono, K., Ono, M., Sasaki, K., Yamaguchi, H., Narumi, T., Tanaka, T., Sugiyama, M., Yamamura, T., Terakawa, T., Gion, K., Suzuri, R., Tanaka, Y., Nakatsuka, T., Kimura, S., Nishihara, M., Sakai, T., Endo-Onodera, R., Saitoh, K., Isuzugawa, K., Oshima, Y., Koyama, T., Ikeda, M., Narukawa, M., Matsui, K., Nakata, M., Ohtsubo, N. and Ohme-Takagi, M. Plant Biotechnology 28: 123-130. [J-STAGE]

11. Genetic engineering of flavonoid pigments to modify flower color in floricultural plants. Nishihara, M. and Nakatsuka, T. Biotechnology Letters 33: 433-441. [PubMed]