2006-2010

1.  UDP-glucose:3-deoxyanthocyanidin 5-O-glucosyltransferase from Sinningia cardinalis. Nakatsuka, T.and Nishihara, M. Planta 232:383-392. 2010. [PubMed].

2. Strict de novo methylation of the 35S enhancer sequence in gentian. Mishiba, K., Yamasaki, S., Nakatsuka, T., Abe, Y.,Daimon, H., Oda, M. and Nishihara, M. PLoS One 5(3): e9670. 2010. [PubMed]

3. Two R2R3-MYB genes, homologues of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of asiatic hybrid lily. Yamagishi, M., Shimoyamada, Y., Nakatsuka, T., Masuda, K. Plant and Cell Physiology: 51: 463-474. 2010. [PubMed]

4. Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian. Nakatsuka, T., Mishiba, K., Kubota, A. Abe, Y., Yamamura, Y., Nakamura, N., Tanaka, Y. and Nishihara, M. Journal of Plant Physiol 167: 231-237. 2010. [PubMed]

5. Genetic engineering of novel flower colors in floricultural plants: Recent advances via transgenic approaches. Nishihara, M. and Nakatsuka, T. Methods in Molecular Biology 589: Protocols for in vitro propagation of ornamental plants. S. Mohan Jain and Sergio J. Ochatt (eds). The Humana Press Inc. pp. 325-347. 2010. [PubMed]

6. Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content. Mishiba, K., Yamane, K., Nakatsuka, T., Nakano, Y., Yamamura, S., Abe, J., Kawamura, K., Takahata, Y. and Nishihara, M. Breeding Science 59: 119-127. 2009. [J-STAGE]

7. Over-expression of Arabidopsis FT gene reduces juvenile phase and induces early fowering in ornamental gentian plants. Nakatsuka, T., Abe, Y., Kakizaki, Y., Kubota, A., Shimada, N. and Nishihara, M. Euphytica 168: 113-119. 2009. [SpringerLink]

8. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi, H., Munemura, I., Nakatsuka, T., Nishihara, M. and Uchimiya, H. 2009. The Journal of Horticultural Science and Biotechnology 84: 312-318. 2009. [LINK]

9. Development of codominant DNA marker distinguishing pink from blue flowers in Gentiana scabra. Kakizaki, Y., Nakatsuka, T., Kawamura, H., Abe, J., Abe, Y., Yamamura S. and Nishihara, M. Breeding Research 11: 9-14. 2009. (in Japanese with English abstract) [J-STAGE]

10. Identification of gentian cultivars using SCAR markers based on intron-length polymorphisms of flavonoid biosynthetic genes. Shimada, N., Nakatsuka, T., Nakano, Y., Kakizaki, Y., Abe, Y., Hikage, T. and Nishihara, M. Scientia Horticulturae 119: 292-296. 2009. [ScienceDirect]

11. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi H, Munemura I, Nakatsuka T, Nishihara M, and Uchimiya H.J. Hortic. Sci. Biotechnol. 84, 113-119. 2009. [Link]

1. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Nakatsuka, T., Haruta, K.S., Pitaksutheepong, C., Abe, Y., Kakizaki, Y., Yamamoto, K., Shimada, N., Yamamura, S. and Nishihara, M. Plant and Cell Physiology 49: 1818-1829. 2008. [PubMed]

2. Cloning and characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian. Nakatsuka, T., Sato, K., Takahashi, H., Yamamura, S., and Nishihara, M. Journal of Experimental Botany 59: 1241-1252. 2008. [PubMed]

3. RIP and RALyase cleave the sarcin/ricin domain, a critical domain for ribosome function, during senescence of wheat coleoptiles. Sawasaki, T., Nishihara, M. and Endo, Y. Biochemical and Biophysical Research Communications 370: 561-565. 2008. [SpringerLink]

4. Flower color modification of gentian plants by RNAi-mediated gene silencing. Nakatsuka, T., Mishiba, K., Abe, Y., Kubota, A., Kakizaki, Y., Yamamura, S. and Nishihara, M. Plant Biotechnology 25: 61-68. 2008. [J-STAGE]

5. FioreDB: a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants. Mitsuda, N., Umemura, Y. Ikeda, M., Shikata, M., Koyama, T., Matsui,K., Narumi, T., Aida, R., Sasaki, K., Hiyama, T., Higuchi, T., Ono, M., Isuzugawa, K., Saitoh, K., Endo,R., Ikeda, K., Nakatsuka, T., Nishihara, M., Yamamura, S., Yamamura, T., Terakawa, T., Ohtsubo, N., and Ohme-Takagi, M. Plant Biotechnology 25: 37-44. 2008. [J-STAGE]

6. Gentians: From gene cloning to molecular breeding.  Nishihara, M., Nakatsuka, T., Mizutani-Fukuchi, M., Tanaka, Y., Yamamura, S. Floricultural and Ornamental Biotechnology V, published by Global Science Books, UK. pp. 57-67. 2008.

7. Induction of differential flower pigmentation patterns by RNAi using promoters with distinct tissue-specific activity. Nakatsuka, T., Pitaksutheepong, C., Yamamura, S. and Nishihara, M. Plant Biotechnology Reports 1: 251-257. 2007. [SpringerLink]

8. Pathogenesis-related protein 1 homologue is an antifungal protein in Wasabia japonica leaves and confers resistance to Botrytis cinerea in transgenic tobacco. Kiba, A., Nishihara, M., Nakatsuka , T. and Yamamura, S. Plant Biotechnology 24: 247-253. 2007. [J-STAGE]

9. Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway.Suzuki, S., Nishihara, M,, Nakatsuka, T., Misawa, N., Ogiwara, I. and Yamamura, S. Plant Cell Reports 26: 951-959. 2007.  [PubMed]

10. Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes. Nakatsuka, T., Abe, Y., Kakizaki, Y., Yamamura, S. and Nishihara, M.  Plant Cell Reports 26: 1951-1959. 2007. [PubMed]

11. Two different transposable elements inserted in flavonoid 3', 5'-hydroxylase gene contribute to pink flower coloration in Gentiana scabra. Nakatsuka, T., Nishihara, M., Mishiba, K., Hirano, H. and Yamamura, S. Molecular Genetics and Genomics 275: 231-241. 2006. [PubMed]

12. Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants. Nakatsuka, T., Nishihara, M., Mishiba, K. and Yamamura, S. Molecular Breeding 17: 91-99. 2006. [SpringerLink]

13. Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants. Nishihara, M., Nakatsuka, T., Hosokawa, K., Yokoi, T., Abe, Y., Mishiba, K. and Yamamura S. Plant Biotechnology 23: 25-32. 2006. [J-STAGE]

14. Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes. Mishiba, K., Nishihara, M., Abe, Y., Nakatsuka, T., Kawamura, H., Kodama, K., Takesawa, T., Abe, J. and Yamamura, S. Plant Biotechnology 23: 33-38. 2006. [J-STAGE]

15. Isolation and characterization of a cDNA encoding polyketide reductase in Lotus japonicus. Shimada, N., Nakatsuka, T., Nishihara, M., Yamamura, S., Ayabe, S. and Aoki, T. Plant Biotechnology 23: 509-513. 2006. [J-STAGE]

16. Transgenic potatoes expressing wasabi defensin peptide confers partial resistanceto gray mold (Botrytis cinerea). Khan, RS., Nishihara, M., Yamamura, S., Nakamura, I. and Mii, M. Plant Biotechnology 23: 179-183. 2006. [J-STAGE]

2001-2005

1. Temporal expression of flavonoid biosynthesis-related genes regulates flower pigmentation in gentian plants. Nakatsuka, T., Nishihara, M., Mishiba, K. and Yamamura, S. Plant Science 168: 1309-1318. 2005 [ScienceDirect].

2. Two different mutations are involved in the formation of white-flowered gentian plants. Nakatsuka,T., Nishihara, M., Mishiba, K. and Yamamura, S. Plant Science 169: 949-958. 2005. [ScienceDirect]

3. Consistent transcriptional silencing of 35S-driven transgenes in gentian. Mishiba, K., Nishihara, M., Nakatsuka, T., Abe, Y., Hirano, H., Yokoi, T., Kikuchi, A. and Yamamura, S. The Plant Journal 44: 541-556. 2005. [PubMed]

4. Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. Nishihara, M., Nakatsuka, T. and Yamamura, S. FEBS Letters 579: 6074-6078. 2005. [PubMed]

5. A peroxiredoxin Q homolog from gentians is involved in both resistance against fungal disease and oxidative stress. Kiba, H., Nishihara, M., Tsukatani, N., Nakatsuka, T., Kato, Y. and Yamamura, S. Plant and Cell Physiology 46: 1007-1015. 2005. [PubMed]

6. Comparison of selection efficiency between the bar and hpt genes in Agrobacterium-mediated transformation of Muscari armeniacum. Suzuki, S., Nakano, M., Koike, Y., Ueda, K., Inoue, M., Nishihara, M. and Yamamura, S. Journal of the Japanese Society for Horticultura Science 74: 60-62. 2005. [PDF]

7. Transgenic rice expressing wasabi defensin gene exhibit its enhanced resistance to blast fungus (Magnaporthe grisea). Kanzaki, H., Nirasawa, S., Saito, H., Ito, M., Nishihara, M., Yamamura, S., Suzuki, K., Terauchi, R. and Nakamura I. In '' Rice Blast:Interaction with Rice and Control. " by eds. Shinji Kawasaki. Kluwer Academic. 2004.

8. cDNAs for the synthesis of cyclic carotenoids in petals of Gentiana lutea and their transcriptional regulation during flower development. Zhu C. F., Yamamura, S., Nishihara, M., Koiwa, H. and Sandmann G. Biochimica et Biophysica Acta 1625: 305-308. 2003. [PubMed]

9. C-terminal domain of a hevein-like protein from Wasabia japonica has potent antimicrobial activity. Kiba, A., Saitoh, H., Nishihara, M., Omiya, K. and Yamamura, S. Plant and Cell Physiology 44: 296-303. 2003. [PubMed]

10. cDNA cloning and expression of carotenogenic genes during flower development in Gentiana lutea. Zhu, C.F., Yamamura, S., Koiwa, H., Nishihara, M. and Sandmann, G. Plant Molecular Biology 48:277-285. 2002. [PubMed]

11. Production of antimicrobial defension in Nicotiana benthamiana with a potato virus vector. Saitoh, H., Kiba, A., Nishihara, M., Yamamura, S., Suzuki, K. and Terauchi, R. Molecular Plant-Microbe Interaction 14: 111-115. 2001. [PubMed]

1994-2000

1. Morikawa, H., Irifune, K., Seki, M., Nishihara, M., and Tanaka, T. (1992) Bombardment-mediated gene delivery into and expression in walled cells of plants. Proceedings of the second Sino-Japanese symposium on plant chromosomes. Plant Chromosome Research.p1-7.

2. Nishihara,  M., Ito, M., Tanaka, I., Kyo, M., Ono, K.,  Irifune,  K., and Morikawa, H. (1993) Expression of the  beta-glucuronidase  gene in pollen of lily (Lilium longiflorum), tobacco (Nicotiana tabacum), Nicotiana rustica, and peony (Paeonia lactiflora)  by  particle bombardment.  Plant Physiol. 102: 357-361.

3. Morikawa, H., Nishihara, M., Seki, M., and Irifune, K. (1994)    Bombardment-mediated  transformation of plant cells.  J.  Plant  Res. 107: 117-123.

4. Nishihara, M., Seki, M., Kyo, M., Irifune, K., and Morikawa, H. (1995) Transgenic haploid plants of Nicotiana rustica produced by bombardment-mediated transformation.  Transgenic Res. 4: 341-348.

5. Tanaka, T., Nishihara, M., Seki, M., Sakamoto, A., Tanaka, K., Irifune, K., and Morikawa, H.  (1995) Successful expression in pollen  of various plant species of in  vitro synthesized mRNA introduced by particle bombardment. Plant Mol. Biol.  28: 337-341.

6. Yamamoto, S., Nishihara, M., Morikawa, H., Yamauchi, D., and Minamikawa, T. (1995) Promoter analysis of seed storage protein genes from Canavalia gladiata D.C. Plant Mol. Biol. 27:729-741

7. Takahashi, M., Nishihara, M., Yamamura, S., Nishizawa, S., Irifune, K. and Morikawa, H. (1998) Stable transformation of Eustoma grandiflorum by particle bombardment. Plant Cell Rep. 17:504-507