Overview of Status of Analysis, Design, Fabrication, Tests, etc.
Experiments on smearing method
Wedging
Tape smearing
Air
Spinning
Accomplishments from Previous Week
Clarified the project priorities
Planned risk reduction(feasibility and potential problems of each smearing mechanism)
Goals for Next Week (list names after each item). Use specific and measurable objectives.
Finish risk reduction
Evaluation of the feasibility of each smearing mechanisms
State the easy to apply standards to discern acceptable smears
Clearly stated problems noticed during the risk reduction experiments
Propose methods to avoid stiction in smearing
Generic evaluation of the complexity of design for each smearing mechanism
Sponsor Comments from Last Meeting and Actions Taken to Address these Comments
Use diluted latex water-based paint for similar viscosity and floating particles as the blood substitute
recommended to accomplish smearing and staining device but with creating own blood smearing device as the higher priority
WoW design(extra credit)
Cap piercing mechanism to reduce human interaction with blood directly.
Rocking device for uniformly distributed blood cells in the sample to smear.
Labeling system for accurate patient information tracking.
Instructor Comments from Last Meeting and Actions Taken to Address these Comments
We should complete the training required for experimenting with blood
We decided to start with blood substitutes and use animal blood afterward. Animal blood is much safer and behaves similarly to human blood for smearing
Mix 1 ml of latex paint with 19 ml of distilled water and mix well. Red blood cells are 7 microns, and the concentration of this material is similar to blood. (Choose blood substitutes based on concentration)
A 1000x image of latex paint that has been diluted 1:20 in water provided by sponsor
Presentation suggestions
Introduction: why do people do blood test?
Provide criteria for good and bad smearing, use pictures to explain.
Use drawing and data to further explain abstract concepts.
Prices of existing machines.
Actions: we have taken photos as we moved forward to help us explain things better to the class next week
Decide the highest priority
Design and build a self-smearing device
Decide which mechanism to use for making the blood smear(wedging or tape)
We have tried smearing by hand with traditional method and with different tapes.
We will make the decision after we finish the risk reduction (Still in Progress)
Risks and Areas of Concern
Stiction in smearing with tape
Unevenly paved tape
Varying tape angle
Tape residue on the slide that could be hard to clean
Mechanisms on cutting the tape, stick the tape on to the slide and remove the tape
Requirement to clean or dispose the spreader slide
Parallel contact between the spreader slide and smear slide
The amount of time for blood to diffuse on the spreader
Resources or Information Required but not Available
(According to the sponsor), the ~$1000 Hemaprep machine cannot be purchased.
Microscopes
We are supposed to check the quality of smearing with 1000x microscope
Possible available resources on campus
Take slides to Moores Cancer Center
http://sailorgroup.ucsd.edu/info/equipment.html ($42/hr, Pacific Hall)
https://humanstemcellcore.ucsd.edu/Microscopes%20AYL.html ($32/hr, 2880 Torrey Pines Scenic Drive)
Cannot find policies regarding the use of blood samples on campus
Schedule
Describe upcoming milestone
Prototype of self-smearing with tape mechanism
Gantt Chart for upcoming week
Budget (list amount spent and amount remaining)
3 kinds of Tape for testing blood smearing
Packing Tape ($1.99)
Duct Tape ($2.99)
Masking Tape ($6.99)
a vial of 7-micron polystyrene bead control material, ($80.00)
PPS-4
Polystyrene Particle Size Standardt, Flow Cytometry Grade
Progress on Report and Webpage
Webpage link: https://sites.google.com/a/eng.ucsd.edu/156b-2019-winter-team06/
Personal Information of each team member has been uploaded
Team management page has been updated with pictures and Gantt chart
Home page has been updated with pictures, background info and objective.