If 6.0 milligrams of nitrogen is added for 2,000 milliliters of water, then the mutant plants will have 5% bigger rosette leaves, 5% taller bolt heights, and 2% more siliques will sprout from the main bolt.
Our variable is Nitrogen and the AAP6 gene. We created a urea solution with 6.0 milligrams of nitrogen in 2,000 milliliters of water to treat our plants once a week. Our Arabidopsis plants are treated with four drops of urea. Each drop is 1 millimeter. The independent variable for this experiment is the absence or presence of a treatment of Urea and the AAP6 gene. The dependent variable is the growth of the plant as a result of this change.
Urea was chosen because it increases cellular reproduction and photosynthesis rates. It is also important for the creation of amino acids and proteins which is important for both DNA and RNA. Urea promotes strong stems and lush rosettes for Arabidopsis Thaliana as well.
In this experiment, we were observing the differences between mutant plants and wild type plants when the variable of urea was added. Urea is often used as a fertilizer, and the AAP6 gene is present in all major crop plants. Being able to see how they interact with each other and what promotes the best growth is the main focus. The mutant plants have the AAP6 gene silenced in its genome, and the wild type plants have the AAP6 gene present in its genome. Throughout the experiment we measured the growth of the plants. Once the plants went thought their lifecycle, we observed and compared the results to disprove our hypothesis.
We are Fluvanna's first block class for BRVGS AP Biology. This 14-person class has many important roles to complete this research project. Our presenters include: Timothy, Greg, Hyden, and Dean. Our people collecting data and creating graphs of everyone's numbers include: Jack, Sophie, Anthony, and Emma. Our people working on our poster include: Amaris, Wesley, and Dashari. Lastly, our people working on this website include: Valerie, Izabella, and Abby.
FCHS Block 1 Class Photo
We would like to thank Virginia Tech for bringing us this opportunity to help take part in college-level research. We understand the time, resources, and work that is put into this project every year and are thankful that you are taking part in this with us. The presentation given at the beginning of the semester was very helpful for us to complete this project, and we appreciate the time both Dr. David Haak and Ms. Iliana Castillo-Machuca took to put together the information and present it to us.
Sincerely,
Fluvanna Block 1