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1. How do I decide which 400 to use?
The NEO 400 is a more modern instrument than the 400 MR, providing superior sensitivity, line shapes, and ease of use.
The only significant advantage the 400 MR has is a more flexible set of policies around its operation. You should use the 400 MR if you want to run long experiments during daytime hours, or want quick results when there are many experiments in the queue on the NEO 400.
The NEO 400 is generally only operated in automation mode. In some circumstances, the constraints of the automation software may make it easier to run specific experiments on the 400 MR. You should consult with the NMR director if you believe this is the case for an experiment you would like to run, because it may be possible to run the experiment in automation mode after all or to arrange to manually acquire the data on the NEO 400 with assistance.
2. Does it matter which NMR tube I use?
Only if you care about sensitivity and/or running variable temperature experiments. Here is a comparison of different tubes:
For quick and dirty spectra, any of these tubes will work but it is clear that if you are sample limited and want an excellent S/N you should use a better quality tube. (Generally, better quality tubes are more expensive and will not be labeled "high throughput".)
If you want to do variable temperature experiments, it's important to use a good quality tube (Type 1, class A) so that it doesn't break as a result of temperature changes. The only tube shown above that is suitable is the Wilmad 528.
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